Consensus olfactory receptor consOR2 bound to S-carvone and in complex with mini-Gs trimeric protein
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8uy0
Q-score: 0.489
de March CA, Ma N, Billesbolle CB, Tewari J, Llinas Del Torrent C, van der Velden WJC, Ojiro I, Takayama I, Faust B, Li L, Vaidehi N, Manglik A, Matsunami H
Nature (2024) 635 pp. 499-508 [ DOI: doi:10.1038/s41586-024-08126-0 Pubmed: 39478229 ]
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Consor2-minigs complex bound to nb35 (100 kDa, Complex from synthetic construct)
- Consor2 (35 kDa, Protein from synthetic construct)
- Minigs399 (30 kDa, Protein from Homo sapiens)
- (5s)-2-methyl-5-(prop-1-en-2-yl)cyclohex-2-en-1-one (150 Da, Ligand)
CryoEM structure of D2 dopamine receptor in complex with GoA KE mutant, scFv16, and dopamine
Knight KM, Krumm BE, Kapolka NJ, Ludlam WG, Cui M, Mani S, Prytkova I, Obarow EG, Lefevre TJ, Wei W, Ma N, Huang XP, Fay JF, Vaidehi N, Smrcka AV, Slesinger PA, Logothetis DE, Martemyanov KA, Roth BL, Dohlman HG
Nat Commun (2024) 15 pp. 6643-6643 [ DOI: doi:10.1038/s41467-024-50964-z Pubmed: 39103320 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- D(2) dopamine receptor (50 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Human drd2 in complex with heterotrimeric g protein goa (k46e), scfv16, and dopamine (120 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Escherichia coli)
- L-dopamine (153 Da, Ligand)
CryoEM structure of D2 dopamine receptor in complex with GoA KE mutant and dopamine
Knight KM, Krumm BE, Kapolka NJ, Ludlam WG, Cui M, Mani S, Prytkova I, Obarow EG, Lefevre TJ, Wei W, Ma N, Huang XP, Fay JF, Vaidehi N, Smrcka AV, Slesinger PA, Logothetis DE, Martemyanov KA, Roth BL, Dohlman HG
Nat Commun (2024) 15 pp. 6643-6643 [ DOI: doi:10.1038/s41467-024-50964-z Pubmed: 39103320 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- D(2) dopamine receptor (50 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(o) subunit alpha (40 kDa, Protein from Homo sapiens)
- Human drd2 in complex with heterotrimeric g protein goa (k46e) and dopamine (120 kDa, Complex from Homo sapiens)
- L-dopamine (153 Da, Ligand)
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 8pm2
Q-score: 0.496
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Verprintsev DB, Vaidehi N, Weyand SN, Tate CG
bioRxiv (2023) [ DOI: doi:10.1101/2023.07.07.547762 Pubmed: 37461561 ]
- Trace amine-associated receptor 7f (41 kDa, Protein from Mus musculus)
- Cholesterol hemisuccinate (486 Da, Ligand)
- A complex of mouse trace-amine associated receptor 7f solubilized in lmng/chs bound to n,n-dimethylcyclohexylamine and coupled to: engineered guanine nucleotide-binding protein g(s) subunit alpha isoform short + guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 + guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 + nanobody 35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody 35 (16 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- ~{n},~{n}-dimethylcyclohexanamine (127 Da, Ligand)
Human olfactory receptor OR51E2 bound to propionate in complex with miniGs399
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8f76
Q-score: 0.52
Billesbolle CB, de March CA, van der Velden WJC, Ma N, Tewari J, Del Torrent CL, Li L, Faust B, Vaidehi N, Matsunami H, Manglik A
Nature (2023) 615 pp. 742-749 [ DOI: doi:10.1038/s41586-023-05798-y Pubmed: 36922591 ]
- Olfactory receptor 51e2 (36 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Or51e2-gs complex (100 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
- Propanoic acid (74 Da, Ligand)
Structure of the GPCR dimer Ste2 bound to an antagonist
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7qa8
Q-score: 0.538
Velazhahan V, Ma N, Vaidehi N, Tate CG
Nature (2022) 603 pp. 743-748 [ DOI: doi:10.1038/s41586-022-04498-3 Pubmed: 35296853 ]
- Water (18 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Pheromone alpha factor receptor (47 kDa, Protein from Saccharomyces cerevisiae)
- Gpcr dimer ste2 bound to an antagonist (Complex from Saccharomyces cerevisiae)
- His-ala-leu-gln-leu-lys-pro-gly-gln-pro-nle-tyr (1 kDa, Protein from Saccharomyces cerevisiae)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structure of the ligand-free GPCR dimer Ste2
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7qb9
Q-score: 0.501
Velazhahan V, Ma N, Vaidehi N, Tate CG
Nature (2022) 603 pp. 743-748 [ DOI: doi:10.1038/s41586-022-04498-3 Pubmed: 35296853 ]
- Cholesterol hemisuccinate (486 Da, Ligand)
- Structure of the ligand-free gpcr dimer ste2 (Complex from Saccharomyces cerevisiae)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Pheromone alpha factor receptor (47 kDa, Protein from Saccharomyces cerevisiae)
Structure of the GPCR dimer Ste2 in the inactive-like state bound to agonist
Resolution: 3.53 Å
EM Method: Single-particle
Fitted PDBs: 7qbc
Q-score: 0.422
Velazhahan V, Ma N, Vaidehi N, Tate CG
Nature (2022) 603 pp. 743-748 [ DOI: doi:10.1038/s41586-022-04498-3 Pubmed: 35296853 ]
- Gpcr dimer ste2 in the inactive-like state bound to agonist (Complex from Saccharomyces cerevisiae)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Alpha factor pheromone (1 kDa, Protein from Saccharomyces cerevisiae)
- Pheromone alpha factor receptor (47 kDa, Protein from Saccharomyces cerevisiae)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structure of the GPCR dimer Ste2 in the active-like state bound to agonist
Resolution: 3.46 Å
EM Method: Single-particle
Fitted PDBs: 7qbi
Q-score: 0.444
Velazhahan V, Ma N, Vaidehi N, Tate CG
Nature (2022) 603 pp. 743-748 [ DOI: doi:10.1038/s41586-022-04498-3 Pubmed: 35296853 ]
- Cholesterol hemisuccinate (486 Da, Ligand)
- Gpcr dimer ste2 in the active-like state bound to agonist (Complex from Saccharomyces cerevisiae)
- Alpha factor pheromone (1 kDa, Protein from Saccharomyces cerevisiae)
- Pheromone alpha factor receptor (47 kDa, Protein from Saccharomyces cerevisiae)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Class D GPCR Ste2 dimer coupled to two G proteins
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7ad3
Q-score: 0.455
Velazhahan V, Ma N, Pandy-Szekeres G, Kooistra AJ, Lee Y, Gloriam DE, Vaidehi N, Tate CG
Nature (2021) 589 pp. 148-153 [ DOI: doi:10.1038/s41586-020-2994-1 Pubmed: 33268889 ]
- Guanine nucleotide-binding protein alpha-1 subunit,guanine nucleotide-binding protein alpha-1 subunit (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Cholesterol hemisuccinate (486 Da, Ligand)
- Alpha-factor mating pheromone (1 kDa, Protein from Saccharomyces cerevisiae)
- Pheromone alpha factor receptor (47 kDa, Protein from Saccharomyces cerevisiae)
- Guanine nucleotide-binding protein alpha-1 subunit,guanine nucleotide-binding protein alpha-1 subunit (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ste2 dimer coupled to two g proteins (Complex from Saccharomyces cerevisiae)
- Ste4 isoform 1 (46 kDa, Protein from Saccharomyces cerevisiae)
- Ste2 dimer coupled to two g proteins (266 kDa, Complex)
- Guanine nucleotide-binding protein subunit gamma (12 kDa, Protein from Saccharomyces cerevisiae)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Alpha-factor mating pheromone (Complex from Saccharomyces cerevisiae)