Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 8pm2
Q-score: 0.496
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Verprintsev DB, Vaidehi N, Weyand SN, Tate CG
bioRxiv (2023) [ DOI: doi:10.1101/2023.07.07.547762 Pubmed: 37461561 ]
- Trace amine-associated receptor 7f (41 kDa, Protein from Mus musculus)
- Cholesterol hemisuccinate (486 Da, Ligand)
- A complex of mouse trace-amine associated receptor 7f solubilized in lmng/chs bound to n,n-dimethylcyclohexylamine and coupled to: engineered guanine nucleotide-binding protein g(s) subunit alpha isoform short + guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 + guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 + nanobody 35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nanobody 35 (16 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- ~{n},~{n}-dimethylcyclohexanamine (127 Da, Ligand)
Structure of the GPCR dimer Ste2 bound to an antagonist
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7qa8
Q-score: 0.538
Velazhahan V, Ma N, Vaidehi N, Tate CG
Nature (2022) 603 pp. 743-748 [ DOI: doi:10.1038/s41586-022-04498-3 Pubmed: 35296853 ]
- Water (18 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Pheromone alpha factor receptor (47 kDa, Protein from Saccharomyces cerevisiae)
- Gpcr dimer ste2 bound to an antagonist (Complex from Saccharomyces cerevisiae)
- His-ala-leu-gln-leu-lys-pro-gly-gln-pro-nle-tyr (1 kDa, Protein from Saccharomyces cerevisiae)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structure of the ligand-free GPCR dimer Ste2
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7qb9
Q-score: 0.501
Velazhahan V, Ma N, Vaidehi N, Tate CG
Nature (2022) 603 pp. 743-748 [ DOI: doi:10.1038/s41586-022-04498-3 Pubmed: 35296853 ]
- Cholesterol hemisuccinate (486 Da, Ligand)
- Structure of the ligand-free gpcr dimer ste2 (Complex from Saccharomyces cerevisiae)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Pheromone alpha factor receptor (47 kDa, Protein from Saccharomyces cerevisiae)
Structure of the GPCR dimer Ste2 in the inactive-like state bound to agonist
Resolution: 3.53 Å
EM Method: Single-particle
Fitted PDBs: 7qbc
Q-score: 0.422
Velazhahan V, Ma N, Vaidehi N, Tate CG
Nature (2022) 603 pp. 743-748 [ DOI: doi:10.1038/s41586-022-04498-3 Pubmed: 35296853 ]
- Gpcr dimer ste2 in the inactive-like state bound to agonist (Complex from Saccharomyces cerevisiae)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Alpha factor pheromone (1 kDa, Protein from Saccharomyces cerevisiae)
- Pheromone alpha factor receptor (47 kDa, Protein from Saccharomyces cerevisiae)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structure of the GPCR dimer Ste2 in the active-like state bound to agonist
Resolution: 3.46 Å
EM Method: Single-particle
Fitted PDBs: 7qbi
Q-score: 0.444
Velazhahan V, Ma N, Vaidehi N, Tate CG
Nature (2022) 603 pp. 743-748 [ DOI: doi:10.1038/s41586-022-04498-3 Pubmed: 35296853 ]
- Cholesterol hemisuccinate (486 Da, Ligand)
- Gpcr dimer ste2 in the active-like state bound to agonist (Complex from Saccharomyces cerevisiae)
- Alpha factor pheromone (1 kDa, Protein from Saccharomyces cerevisiae)
- Pheromone alpha factor receptor (47 kDa, Protein from Saccharomyces cerevisiae)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)