Phosphorylated human NKCC1_K289NA492E in complex with furosemide
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 9c0e
Q-score: 0.6
Zhao Y, Vidossich P, Forbush B, Ma J, Rinehart J, De Vivo M, Cao E
EMBO J (2025) [ Pubmed: 39875725 DOI: doi:10.1038/s44318-025-00368-6 ]
- Potassium ion (39 Da, Ligand)
- 5-(aminosulfonyl)-4-chloro-2-[(2-furylmethyl)amino]benzoic acid (330 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Phosphorylated nkcc1_k289na492e in complex with furosemide (Complex from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Solute carrier family 12 member 2 (131 kDa, Protein from Homo sapiens)
- Chloride ion (35 Da, Ligand)
Phosphorylated human NKCC1 in complex with torsemide
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 9c0g
Q-score: 0.591
Zhao Y, Vidossich P, Forbush B, Ma J, Rinehart J, De Vivo M, Cao E
EMBO J (2025) [ Pubmed: 39875725 DOI: doi:10.1038/s44318-025-00368-6 ]
- Solute carrier family 12 member 2 (131 kDa, Protein from Homo sapiens)
- Phosphorylated human nkcc1 in complex with torsemide (Complex from Homo sapiens)
- Sodium ion (22 Da, Ligand)
- Water (18 Da, Ligand)
- 4-(3-methylanilino)-n-[(propan-2-yl)carbamoyl]pyridine-3-sulfonamide (348 Da, Ligand)
- Chloride ion (35 Da, Ligand)
Phosphorylated human NKCC1 in complex with bumetanide
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 9c0h
Q-score: 0.602
Zhao Y, Vidossich P, Forbush B, Ma J, Rinehart J, De Vivo M, Cao E
EMBO J (2025) [ Pubmed: 39875725 DOI: doi:10.1038/s44318-025-00368-6 ]
- 3-(butylamino)-4-phenoxy-5-sulfamoylbenzoic acid (364 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Phosphorylated human nkcc1 in complex with bumetanide (Complex from Homo sapiens)
- Solute carrier family 12 member 2 (131 kDa, Protein from Homo sapiens)
- Chloride ion (35 Da, Ligand)
Influenza A/H7N9 polymerase elongation complex
Resolution: 2.48 Å
EM Method: Single-particle
Fitted PDBs: 7qtl
Q-score: 0.611
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ Pubmed: 36596301 DOI: doi:10.1016/j.celrep.2022.111901 ]
- Polymerase basic protein 2 (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 5'-o-[(s)-hydroxy{[(s)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]cytidine (481 Da, Ligand)
- 3' vrna (6 kDa, RNA from Influenza A virus)
- 5' vrna (4 kDa, RNA from Influenza A virus)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- A/h7n9-elongation (255 kDa, Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Mrna (5 kDa, RNA from Influenza A virus)
- Magnesium ion (24 Da, Ligand)
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
- Polymerase acidic protein (82 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Water (18 Da, Ligand)
Resolution: 2.51 Å
EM Method: Single-particle
Fitted PDBs: 7r0e
Q-score: 0.538
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ Pubmed: 36596301 DOI: doi:10.1016/j.celrep.2022.111901 ]
- Polymerase basic protein 2 (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- A/h7n9-backtracked-t/u+1 (255 kDa, Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Mrna (6 kDa, RNA from Influenza A virus)
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 5' vrna (4 kDa, RNA from Influenza A virus)
- Magnesium ion (24 Da, Ligand)
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
- Polymerase acidic protein (82 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- 3' vrna (6 kDa, RNA from Influenza A virus)
- Water (18 Da, Ligand)
Resolution: 2.58 Å
EM Method: Single-particle
Fitted PDBs: 7r1f
Q-score: 0.524
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ Pubmed: 36596301 DOI: doi:10.1016/j.celrep.2022.111901 ]
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Polymerase acidic protein (85 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- B/mem-backtracked (255 kDa, Complex from Influenza B virus (B/Memphis/13/2003))
- 3' vrna (6 kDa, RNA from Influenza B virus)
- 5' vrna (4 kDa, RNA from Influenza B virus)
- Mrna (6 kDa, RNA from Influenza B virus)
- Magnesium ion (24 Da, Ligand)
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
- Water (18 Da, Ligand)
- Polymerase basic protein 2 (90 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
Symmetric dimer of influenza A/H7N9 polymerase bound to 5' vRNA hook
Resolution: 3.12 Å
EM Method: Single-particle
Fitted PDBs: 7zpl
Q-score: 0.481
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ Pubmed: 36596301 DOI: doi:10.1016/j.celrep.2022.111901 ]
- Symmetric dimer of influenza a/h7n9 polymerase bound to 5' vrna hook (510 kDa, Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase basic protein 2 (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Magnesium ion (24 Da, Ligand)
- 5' vrna (4 kDa, RNA from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase acidic protein (82 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
Influenza A/H7N9 polymerase apo-protein dimer complex
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 7zpm
Q-score: 0.517
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ Pubmed: 36596301 DOI: doi:10.1016/j.celrep.2022.111901 ]
- Influenza a/h7n9 polymerase apo-protein dimer complex (510 kDa, Complex from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase acidic protein (82 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Polymerase basic protein 2 (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8bdr
Q-score: 0.565
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ Pubmed: 36596301 DOI: doi:10.1016/j.celrep.2022.111901 ]
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Early transcription elongation state of influenza b/mem polymerase backtracked due to double incoproation of nucleotide analogue t1106 and with singly incoporated t1106 at the u +1 position (Complex from Influenza B virus (B/Memphis/13/2003))
- Polymerase acidic protein (85 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Mrna (5 kDa, RNA from Influenza B virus (B/Kaliningrad/RII06/2012))
- 5' vrna (4 kDa, RNA from Influenza B virus)
- Magnesium ion (24 Da, Ligand)
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
- 3' vrna (6 kDa, RNA from Influenza B virus (B/Acre/117700/2012))
- Polymerase basic protein 2 (90 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
Resolution: 2.34 Å
EM Method: Single-particle
Fitted PDBs: 8be0
Q-score: 0.597
Kouba T, Dubankova A, Drncova P, Donati E, Vidossich P, Speranzini V, Pflug A, Huchting J, Meier C, De Vivo M, Cusack S
Cell Rep (2023) 42 pp. 111901-111901 [ Pubmed: 36596301 DOI: doi:10.1016/j.celrep.2022.111901 ]
- Rna-directed rna polymerase catalytic subunit (86 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Early transcription elongation state of influenza b/mem polymerase backtracked due to double incoproation of nucleotide analogue t1106 and with singly incoporated t1106 at the c +1 position (Complex from Influenza B virus (B/Memphis/13/2003))
- 5' vrna (4 kDa, RNA from Influenza B virus)
- Polymerase acidic protein (85 kDa, Protein from Influenza B virus (B/Memphis/13/2003))
- Magnesium ion (24 Da, Ligand)
- P1-7-methylguanosine-p3-adenosine-5',5'-triphosphate (787 Da, Ligand)
- 3' vrna (6 kDa, RNA from Influenza B virus)
- Mrna (6 kDa, RNA from Influenza B virus)
- Water (18 Da, Ligand)
- Polymerase basic protein 2 (90 kDa, Protein from Influenza B virus (B/Memphis/13/2003))