Cryo-EM structure of the dystrophin glycoprotein complex
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8yt8
Q-score: 0.412
Wan L, Ge X, Xu Q, Huang G, Yang T, Campbell KP, Yan Z, Wu J
Nature (2025) 637 pp. 1252-1260 [ DOI: doi:10.1038/s41586-024-08310-2 Pubmed: 39663450 ]
- Zinc ion (65 Da, Ligand)
- Unknown segment (559 Da, Protein from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Alpha-sarcoglycan (32 kDa, Protein from Mus musculus)
- Sarcospan (19 kDa, Protein from Mus musculus)
- O-[(r)-{[(2r)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-l-serine (792 Da, Ligand)
- Beta-dystroglycan (31 kDa, Protein from Mus musculus)
- Dystrophin (38 kDa, Protein from Mus musculus)
- Beta-sarcoglycan (28 kDa, Protein from Mus musculus)
- Gamma-sarcoglycan (28 kDa, Protein from Mus musculus)
- Dystrobrevin alpha (23 kDa, Protein from Mus musculus)
- Delta-sarcoglycan (29 kDa, Protein from Mus musculus)
- Dystrophin glycoprotein complex, dgc (Complex from Mus musculus)
- Cholesterol (386 Da, Ligand)
Cryo-EM structure of the dystrophin glycoprotein complex
Wan L, Ge X, Xu Q, Huang G, Yang T, Campbell KP, Yan Z, Wu J
Nature (2025) 637 pp. 1252-1260 [ DOI: doi:10.1038/s41586-024-08310-2 Pubmed: 39663450 ]
Cryo_EM map of nucleotide free MlaFEDB complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7cge
Q-score: 0.559
Chi X, Fan Q, Zhang Y, Liang K, Wan L, Zhou Q, Li Y
Cell Res (2020) 30 pp. 1127-1135 [ DOI: doi:10.1038/s41422-020-00404-6 Pubmed: 32884137 ]
- Outer membrane lipid asymmetry maintenance protein mlad (19 kDa, Protein from Escherichia coli (strain K12))
- Phospholipid abc transporter atp-binding protein mlaf (29 kDa, Protein from Escherichia coli (strain K12))
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Lipid asymmetry maintenance abc transporter permease subunit mlae (27 kDa, Protein from Escherichia coli (strain K12))
- Lipid asymmetry maintenance protein mlab (10 kDa, Protein from Escherichia coli (strain K12))
- Cryo_em map of nucleotide free mlafedb complex (Complex from Escherichia coli K-12)
Cryo EM map of the MlaFEDB complex in ATP-bound EQtall conformation (Mutation of E170Q on MlaF)
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 7cgn
Q-score: 0.308
Chi X, Fan Q, Zhang Y, Liang K, Wan L, Zhou Q, Li Y
Cell Res (2020) 30 pp. 1127-1135 [ DOI: doi:10.1038/s41422-020-00404-6 Pubmed: 32884137 ]
- Phospholipid abc transporter atp-binding protein mlaf (29 kDa, Protein from Escherichia coli (strain K12))
- Outer membrane lipid asymmetry maintenance protein mlad (19 kDa, Protein from Escherichia coli (strain K12))
- Lipid asymmetry maintenance abc transporter permease subunit mlae (27 kDa, Protein from Escherichia coli (strain K12))
- Lipid asymmetry maintenance protein mlab (10 kDa, Protein from Escherichia coli (strain K12))
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Cryo em map of the mlafedb complex in atp-bound eqtall conformation (mutation of e170q on mlaf) (Complex from Escherichia coli K-12)
Cryo EM map of the MlaFEDB complex in ATP-bound EQclose conformation (Mutation of E170Q on MlaF)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7ch0
Q-score: 0.487
Chi X, Fan Q, Zhang Y, Liang K, Wan L, Zhou Q, Li Y
Cell Res (2020) 30 pp. 1127-1135 [ DOI: doi:10.1038/s41422-020-00404-6 Pubmed: 32884137 ]
- Phospholipid abc transporter atp-binding protein mlaf (29 kDa, Protein from Escherichia coli K-12)
- Lipid asymmetry maintenance abc transporter permease subunit mlae (27 kDa, Protein from Escherichia coli K-12)
- Lipid asymmetry maintenance protein mlab (10 kDa, Protein from Escherichia coli K-12)
- Outer membrane lipid asymmetry maintenance protein mlad (19 kDa, Protein from Escherichia coli K-12)
- Cryo em map of the mlafedb complex in atp-bound eqclose conformation (mutation of e170q on mlaf) (Complex from Escherichia coli K-12)
- Adenosine-5'-triphosphate (507 Da, Ligand)