Cryo-EM structure of the human glucagon-like peptide-2 receptor-Gs protein complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7d68
Q-score: 0.524
Sun W, Chen LN, Zhou Q, Zhao LH, Yang D, Zhang H, Cong Z, Shen DD, Zhao F, Zhou F, Cai X, Chen Y, Zhou Y, Gadgaard S, van der Velden WJC, Zhao S, Jiang Y, Rosenkilde MM, Xu HE, Zhang Y, Wang MW
Cell Res (2020) 30 pp. 1098-1108 [ DOI: doi:10.1038/s41422-020-00442-0 Pubmed: 33239759 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Bos taurus)
- Pro-glucagon (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Glucagon-like peptide 2 receptor (75 kDa, Protein from Homo sapiens)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Cryo-em structure of the human glucagon-like peptide-2 receptor-gs protein complex (Complex)
- Nanobody-35 (Complex from synthetic construct)
- Glucagon-like peptide 2 receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein (Complex from Bos taurus)
- Water (18 Da, Ligand)
Cryo-EM structure of the human growth hormone-releasing hormone receptor-Gs protein complex
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7cz5
Q-score: 0.582
Zhou F, Zhang H, Cong Z, Zhao LH, Zhou Q, Mao C, Cheng X, Shen DD, Cai X, Ma C, Wang Y, Dai A, Zhou Y, Sun W, Zhao F, Zhao S, Jiang H, Jiang Y, Yang D, Eric Xu H, Zhang Y, Wang MW
Nat Commun (2020) 11 pp. 5205-5205 [ Pubmed: 33060564 DOI: doi:10.1038/s41467-020-18945-0 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (43 kDa, Protein from Rattus norvegicus)
- Nanobody35 (Complex from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Somatoliberin (5 kDa, Protein from Homo sapiens)
- Hormone (Complex from Homo sapiens)
- Growth hormone-releasing hormone receptor,growth hormone-releasing hormone receptor (61 kDa, Protein from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- G(s) subunit beta-1 (Complex from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Growth hormone-releasing hormone receptor - gs protein complex (180 kDa, Complex)
- G(s) subunit gamma-2 (Complex from Bos taurus)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Hormone receptor, g(s) subunit alpha isoforms (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Cholesterol (386 Da, Ligand)
CryoEM Structure of the glucagon receptor with a dual-agonist peptide
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6whc
Q-score: 0.445
Chang R, Zhang X, Qiao A, Dai A, Belousoff MJ, Tan Q, Shao L, Zhong L, Lin G, Liang YL, Ma L, Han S, Yang D, Danev R, Wang MW, Wootten D, Wu B, Sexton PM
J Biol Chem (2020) 295 pp. 9313-9325 [ Pubmed: 32371397 DOI: doi:10.1074/jbc.RA120.013793 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Glucagon receptor (54 kDa, Protein from Homo sapiens)
- Glucagon receptor complex (Complex)
- Dual-agonist peptide (3 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, receptor activity-modifying protein 3, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, calcitonin gene-related peptide type 1 receptor, glucagon receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Dual-agonist peptide (Complex from synthetic construct)
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
Cryo-EM structure of the human glucagon receptor in complex with Gs
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6lmk
Q-score: 0.443
Qiao A, Han S, Li X, Li Z, Zhao P, Dai A, Chang R, Tai L, Tan Q, Chu X, Ma L, Thorsen TS, Reedtz-Runge S, Yang D, Wang MW, Sexton PM, Wootten D, Sun F, Zhao Q, Wu B
Science (2020) 367 pp. 1346-1352 [ Pubmed: 32193322 DOI: doi:10.1126/science.aaz5346 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nb35 (15 kDa, Protein from Lama glama)
- Glucagon (3 kDa, Protein from Homo sapiens)
- Complex of glucagon receptor bound to glucagon, gs protein and nanobody (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Glucagon receptor (48 kDa, Protein from Homo sapiens)
Cryo-EM structure of the human glucagon receptor in complex with Gi1
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6lml
Q-score: 0.425
Qiao A, Han S, Li X, Li Z, Zhao P, Dai A, Chang R, Tai L, Tan Q, Chu X, Ma L, Thorsen TS, Reedtz-Runge S, Yang D, Wang MW, Sexton PM, Wootten D, Sun F, Zhao Q, Wu B
Science (2020) 367 pp. 1346-1352 [ Pubmed: 32193322 DOI: doi:10.1126/science.aaz5346 ]
- Scfv16 (26 kDa, Protein from Mus musculus)
- Glucagon (3 kDa, Protein from Homo sapiens)
- Glucagon receptor (48 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Complex of glucagon receptor bound to glucagon, gi1 protein and antibody (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Cryo-EM of GLP-1 receptor bound to TT-OAD2 non-peptidic agonist
Zhao P, Liang YL, Belousoff MJ, Deganutti G, Fletcher MM, Willard FS, Bell MG, Christe ME, Sloop KW, Inoue A, Truong TT, Clydesdale L, Furness SGB, Christopoulos A, Wang MW, Miller LJ, Reynolds CA, Danev R, Sexton PM, Wootten D
Nature (2020) 577 pp. 432-436 [ Pubmed: 31915381 DOI: 10.1038/s41586-019-1902-z ]
Image sets:
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6pb0
Q-score: 0.455
Ma S, Shen Q, Zhao LH, Mao C, Zhou XE, Shen DD, de Waal PW, Bi P, Li C, Jiang Y, Wang MW, Sexton PM, Wootten D, Melcher K, Zhang Y, Xu HE
Mol Cell (2020) 77 pp. 669- [ DOI: doi:10.1016/j.molcel.2020.01.013 Pubmed: 32004470 ]
- Urocortin (4 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from synthetic construct)
- Palmitic acid (256 Da, Ligand)
- Urocortin1-bound crf1r in complex gs and nb35 (Complex from Homo sapiens)
- Corticotropin-releasing factor receptor 1 (43 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 6pb1
Q-score: 0.47
Ma S, Shen Q, Zhao LH, Mao C, Zhou XE, Shen DD, de Waal PW, Bi P, Li C, Jiang Y, Wang MW, Sexton PM, Wootten D, Melcher K, Zhang Y, Xu HE
Mol Cell (2020) 77 pp. 669- [ DOI: doi:10.1016/j.molcel.2020.01.013 Pubmed: 32004470 ]
- Urocortin (4 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from synthetic construct)
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Urocortin 1-bound corticotropin-releasing factor 2 receptor in complex with gs protein (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
- Corticotropin-releasing factor receptor 2 (45 kDa, Protein from Homo sapiens)
Non-peptide agonist (TT-OAD2) bound to the Glucagon-Like peptide-1 (GLP-1) Receptor
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6orv
Q-score: 0.538
Zhao P, Liang YL, Belousoff MJ, Deganutti G, Fletcher MM, Willard FS, Bell MG, Christe ME, Sloop KW, Inoue A, Truong TT, Clydesdale L, Furness SGB, Christopoulos A, Wang MW, Miller LJ, Reynolds CA, Danev R, Sexton PM, Wootten D
Nature (2020) 577 pp. 432-436 [ DOI: doi:10.1038/s41586-019-1902-z Pubmed: 31915381 ]
- Nanobody 35 (15 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- N-{(3s,8s)-3-{4-[(3,4-dichlorophenyl)methoxy]phenyl}-7-[(1s)-1-phenylpropyl]-2,3,6,7,8,9-hexahydro[1,4]dioxino[2,3-g]isoquinoline-8-carbonyl}-4-(2,3-dimethylpyridin-4-yl)-l-phenylalanine (856 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, glucagon-like peptide 1 receptor (Complex from Homo sapiens)
- Nanobody 35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Tt-oad2:glp-1r:gs complex (120 kDa, Complex)