cryo-EM structure of NTSR1-GRK2-Galpha(q) complexes 1
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 8jpb
Q-score: 0.448
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ Pubmed: 37532940 DOI: doi:10.1038/s41586-023-06395-9 ]
- Nts(8-13) (819 Da, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(q) subunit alpha (41 kDa, Protein from Homo sapiens)
- Staurosporine (466 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 2-[{2-(1-fluorocyclopropyl)-4-[4-(2-methoxyphenyl)piperidin-1-yl]quinazolin-6-yl}(methyl)amino]ethan-1-ol (450 Da, Ligand)
- Ntsr1-grk2-galpha(q) complexes 1 (Complex from Homo sapiens)
- Neurotensin receptor type 1 (46 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Beta-adrenergic receptor kinase 1 (79 kDa, Protein from Bos taurus)
- Tetrafluoroaluminate ion (102 Da, Ligand)
cryo-EM structure of NTSR1-GRK2-Galpha(q) complexes 2
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 8jpc
Q-score: 0.458
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ Pubmed: 37532940 DOI: doi:10.1038/s41586-023-06395-9 ]
- Nts(8-13) (819 Da, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(q) subunit alpha (41 kDa, Protein from Homo sapiens)
- Staurosporine (466 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Ntsr1-grk2-galpha(q) complexes 2 (Complex from Homo sapiens)
- 2-[{2-(1-fluorocyclopropyl)-4-[4-(2-methoxyphenyl)piperidin-1-yl]quinazolin-6-yl}(methyl)amino]ethan-1-ol (450 Da, Ligand)
- Neurotensin receptor type 1 (46 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Beta-adrenergic receptor kinase 1 (79 kDa, Protein from Bos taurus)
- Tetrafluoroaluminate ion (102 Da, Ligand)
Focused refinement structure of GRK2 in NTSR1-GRK2-Galpha(q) complexes
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 8jpd
Q-score: 0.497
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ Pubmed: 37532940 DOI: doi:10.1038/s41586-023-06395-9 ]
- Focused refinement structure of grk2 in ntsr1-grk2-galpha(q) complexes (Complex from Bos taurus)
- Beta-adrenergic receptor kinase 1 (79 kDa, Protein from Bos taurus)
- Staurosporine (466 Da, Ligand)
Focused refinement structure of Galpha(q) in NTSR1-GRK2-Galpha(q) complexes
Resolution: 2.91 Å
EM Method: Single-particle
Fitted PDBs: 8jpe
Q-score: 0.447
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ Pubmed: 37532940 DOI: doi:10.1038/s41586-023-06395-9 ]
- Guanine nucleotide-binding protein g(q) subunit alpha (41 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Focused refinement structure of galpha(q) in ntsr1-grk2-galpha(q) complexes (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Tetrafluoroaluminate ion (102 Da, Ligand)
Focused refiment structure of NTSR1 in NTSR1-GRK2-Galpha(q) complexes
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 8jpf
Q-score: 0.479
Duan J, Liu H, Zhao F, Yuan Q, Ji Y, Cai X, He X, Li X, Li J, Wu K, Gao T, Zhu S, Lin S, Wang MW, Cheng X, Yin W, Jiang Y, Yang D, Xu HE
Nature (2023) 620 pp. 676-681 [ Pubmed: 37532940 DOI: doi:10.1038/s41586-023-06395-9 ]
- Focused refiment structure of ntsr1 in ntsr1-grk2-galpha(q) complexes (Complex from Homo sapiens)
- Neurotensin receptor type 1 (46 kDa, Protein from Homo sapiens)
- Nts (819 Da, Protein from synthetic construct)
- 2-[{2-(1-fluorocyclopropyl)-4-[4-(2-methoxyphenyl)piperidin-1-yl]quinazolin-6-yl}(methyl)amino]ethan-1-ol (450 Da, Ligand)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7eib
Q-score: 0.481
Yin YL, Ye C, Zhou F, Wang J, Yang D, Yin W, Wang MW, Xu HE, Jiang Y
Nat Struct Mol Biol (2021) 28 pp. 755-761 [ Pubmed: 34518695 DOI: doi:10.1038/s41594-021-00645-y ]
- Cryo-em structure of the type 1 bradykinin receptor in complex with the des-arg10-kallidin and an gq protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Bos taurus)
- Type 1 bradykinin receptor (Complex)
- G subunit q (gi2-mini-gq chimeric) (Complex from Rattus norvegicus)
- B1 bradykinin receptor (69 kDa, Protein from Homo sapiens)
- G subunit q (gi1-gq chimeric) (41 kDa, Protein from Homo sapiens)
- Lys-arg-pro-pro-gly-phe-ser-pro-phe (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Rattus norvegicus)
- Lys-arg-pro-pro-gly-phe-ser-pro-phe (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex)
Cryo-EM structure of the type 2 bradykinin receptor in complex with the bradykinin and an Gq protein
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7f2o
Q-score: 0.436
Yin YL, Ye C, Zhou F, Wang J, Yang D, Yin W, Wang MW, Xu HE, Jiang Y
Nat Struct Mol Biol (2021) 28 pp. 755-761 [ Pubmed: 34518695 DOI: doi:10.1038/s41594-021-00645-y ]
- Arg-pro-pro-gly-phe-ser-pro-phe-arg (1 kDa, Protein from Homo sapiens)
- Single fab chain (svfv16) (26 kDa, Protein from synthetic construct)
- G subunit q (gi1-gq chimeric) (41 kDa, Protein from Homo sapiens)
- B2 bradykinin receptor (67 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Arg-pro-pro-gly-phe-ser-pro-phe-arg (Complex from Rattus norvegicus)
- Cryo-em structure of the type 2 bradykinin receptor in complex with the bradykinin and an gq protein (Complex from Homo sapiens)
- Single fab chain (svfv16) (Complex from Bos taurus)
- Type 2 bradykinin receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Rattus norvegicus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex)
- G subunit q (gi2-mini-gq chimeric) (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex)
Cryo-EM structure of alpha-MSH-bound melanocortin-1 receptor in complex with Gs protein and Nb35
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7f4d
Q-score: 0.478
Ma S, Chen Y, Dai A, Yin W, Guo J, Yang D, Zhou F, Jiang Y, Wang MW, Xu HE
Cell Res (2021) 31 pp. 1061-1071 [ DOI: doi:10.1038/s41422-021-00557-y Pubmed: 34453129 ]
- Cryo-em structure of alpha-msh-bound melanocortin-1 receptor in complex with gs protein and nb35 (Complex from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Nanobody 35 (17 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Alpha-melanocyte-stimulating hormone (1 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (Complex from synthetic construct)
- Melanocortin-1 receptor (68 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Melanocortin-1 receptor (Complex)
- Alpha-melanocyte-stimulating hormone (Complex)
- Nanobody 35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (41 kDa, Protein from Homo sapiens)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7f4f
Q-score: 0.509
Ma S, Chen Y, Dai A, Yin W, Guo J, Yang D, Zhou F, Jiang Y, Wang MW, Xu HE
Cell Res (2021) 31 pp. 1061-1071 [ DOI: doi:10.1038/s41422-021-00557-y Pubmed: 34453129 ]
- Cryo-em structure of afamelanotide-bound melanocortin-1 receptor in complex with gs protein and scfv16 (Complex from synthetic construct)
- Calcium ion (40 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Homo sapiens)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Afamelanotide (1 kDa, Protein from synthetic construct)
- Melanocortin-1 receptor (68 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (Complex from Homo sapiens)
- Melanocortin-1 receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (41 kDa, Protein from Homo sapiens)
- Afamelanotide (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7f4h
Q-score: 0.52
Ma S, Chen Y, Dai A, Yin W, Guo J, Yang D, Zhou F, Jiang Y, Wang MW, Xu HE
Cell Res (2021) 31 pp. 1061-1071 [ DOI: doi:10.1038/s41422-021-00557-y Pubmed: 34453129 ]
- Cryo-em structure of afamelanotide-bound melanocortin-1 receptor in complex with gs protein, nb35 and scfv16 (Complex from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Nanobody 35 (17 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (Complex from synthetic construct)
- Scfv16 (Complex)
- Afamelanotide (1 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Melanocortin-1 receptor (68 kDa, Protein from Homo sapiens)
- Nanobody 35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (41 kDa, Protein from Homo sapiens)
- Afamelanotide (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Melanocortin-1 receptor (Complex from synthetic construct)