Cryo-EM structure of E. coli FimH lectin domain bound to Fabs 440-2 and 454-3
Silmon de Monerri NC, Che Y, Lees JA, Jasti J, Wu H, Griffor MC, Kodali S, Hawkins JC, Lypowy J, Ponce C, Curley K, Esadze A, Carcamo J, McLellan T, Keeney D, Illenberger A, Matsuka YV, Shanker S, Chorro L, Gribenko AV, Han S, Anderson AS, Donald RGK
PLoS Pathog (2025) 21 pp. e1012325-e1012325 [ Pubmed: 39970181 DOI: doi:10.1371/journal.ppat.1012325 ]
- 440-2 fab light chain (24 kDa, Protein from Mus musculus)
- 440-2 fab heavy chain (24 kDa, Protein from Mus musculus)
- 445-3 fab heavy chain (24 kDa, Protein from Mus musculus)
- 445-3 fab light chain (23 kDa, Protein from Mus musculus)
- Type 1 fimbrin d-mannose specific adhesin fimh, donor strand complemented with fimg peptide 'triple mutant' (32 kDa, Protein from Escherichia coli)
- Fimh-dsg tm in complex with fabs 440-2 and 445-3 (Complex)
- Fimh-dsg tm (Complex from Escherichia coli)
- Fab 445-3 (Complex from Mus musculus)
- Fab 440-2 (Complex from Mus musculus)
Cryo-EM structure of E. coli FimH lectin domain bound to Fabs 329-2 and 454-3
Silmon de Monerri NC, Che Y, Lees JA, Jasti J, Wu H, Griffor MC, Kodali S, Hawkins JC, Lypowy J, Ponce C, Curley K, Esadze A, Carcamo J, McLellan T, Keeney D, Illenberger A, Matsuka YV, Shanker S, Chorro L, Gribenko AV, Han S, Anderson AS, Donald RGK
PLoS Pathog (2025) 21 pp. e1012325-e1012325 [ Pubmed: 39970181 DOI: doi:10.1371/journal.ppat.1012325 ]
- Fab 329-2 light chain (23 kDa, Protein from Mus musculus)
- Ternary complex of e. coli fimh with fabs 329-2 and 454-3 (Complex from Escherichia coli)
- Type 1 fimbrin d-mannose specific adhesin fimh, donor strand complemented with fimg peptide 'triple mutant' (32 kDa, Protein from Escherichia coli)
- Fab 329-2 heavy chain (24 kDa, Protein from Mus musculus)
- Fab 454-3 light chain (23 kDa, Protein from Mus musculus)
- Fab 454-3 heavy chain (23 kDa, Protein from Mus musculus)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 9cjk
Q-score: 0.344
Xiao L, Pi X, Goss AC, El-Baba T, Ehrmann JF, Grinkevich E, Bazua-Valenti S, Padovano V, Alper SL, Carey D, Udeshi ND, Carr SA, Pablo JL, Robinson CV, Greka A, Wu H
Sci Adv (2024) 10 pp. eadp2221-eadp2221 [ DOI: doi:10.1126/sciadv.adp2221 Pubmed: 39303030 ]
- Tmed9 octamer (Complex from Homo sapiens)
- Transmembrane emp24 domain-containing protein 9 (27 kDa, Protein from Homo sapiens)
- [(2r)-2-[(e)-octadec-9-enoyl]oxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] (e)-octadec-9-enoate (1 kDa, Ligand)
Molecular basis of TMED9 dodecamer
Resolution: 5.5 Å
EM Method: Single-particle
Fitted PDBs: 9cjl
Q-score: 0.128
Xiao L, Pi X, Goss AC, El-Baba T, Ehrmann JF, Grinkevich E, Bazua-Valenti S, Padovano V, Alper SL, Carey D, Udeshi ND, Carr SA, Pablo JL, Robinson CV, Greka A, Wu H
Sci Adv (2024) 10 pp. eadp2221-eadp2221 [ DOI: doi:10.1126/sciadv.adp2221 Pubmed: 39303030 ]
- Transmembrane emp24 domain-containing protein 9 (27 kDa, Protein from Homo sapiens)
- [(2r)-2-[(e)-octadec-9-enoyl]oxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] (e)-octadec-9-enoate (1 kDa, Ligand)
- Tmed9 dodecamer (Complex from Homo sapiens)
Cryo-EM structure of CCR6 bound by SQA1 and OXM2
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 9d3e
Q-score: 0.499
Wasilko DJ, Gerstenberger BS, Farley KA, Li W, Alley J, Schnute ME, Unwalla RJ, Victorino J, Crouse KK, Ding R, Sahasrabudhe PV, Vincent F, Frisbie RK, Dermenci A, Flick A, Choi C, Chinigo G, Mousseau JJ, Trujillo JI, Nuhant P, Mondal P, Lombardo V, Lamb D, Hogan BJ, Minhas GS, Segala E, Oswald C, Windsor IW, Han S, Rappas M, Cooke RM, Calabrese MF, Berstein G, Thorarensen A, Wu H
Nat Commun (2024) 15 pp. 7574-7574 [ DOI: doi:10.1038/s41467-024-52045-7 Pubmed: 39217154 ]
- Ccr6-bril/fab/nb in complex with sqa1 and oxm2 (120 MDa, Complex from Homo sapiens)
- Human ccr6 (54 kDa, Protein from Homo sapiens)
- Anti-bril fab nanobody (15 kDa, Protein from synthetic construct)
- Anti-bril fab light chain (25 kDa, Protein from Homo sapiens)
- N-{[(2r)-4-(bicyclo[1.1.1]pentan-1-yl)-5-oxomorpholin-2-yl]methyl}-1-[4-(trifluoromethyl)phenyl]cyclopropane-1-carboxamide (408 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- 4-[[3,4-bis(oxidanylidene)-2-[[(1~{r})-1-(4-propan-2-ylfuran-2-yl)propyl]amino]cyclobuten-1-yl]amino]-~{n},~{n}-dimethyl-3-oxidanyl-pyridine-2-carboxamide (426 Da, Ligand)
- Anti-bril fab heavy chain (28 kDa, Protein from Homo sapiens)
Cryo-EM structure of CCR6 bound by SQA1 and OXM1
Wasilko DJ, Gerstenberger BS, Farley KA, Li W, Alley J, Schnute ME, Unwalla RJ, Victorino J, Crouse KK, Ding R, Sahasrabudhe PV, Vincent F, Frisbie RK, Dermenci A, Flick A, Choi C, Chinigo G, Mousseau JJ, Trujillo JI, Nuhant P, Mondal P, Lombardo V, Lamb D, Hogan BJ, Minhas GS, Segala E, Oswald C, Windsor IW, Han S, Rappas M, Cooke RM, Calabrese MF, Berstein G, Thorarensen A, Wu H
Nat Commun (2024) 15 pp. 7574-7574 [ DOI: doi:10.1038/s41467-024-52045-7 Pubmed: 39217154 ]
- Ccr6, soluble cytochrome b562 (54 kDa, Protein from Homo sapiens)
- 1-(4-chlorophenyl)-n-{[(2r)-4-(2,3-dihydro-1h-inden-2-yl)-5-oxomorpholin-2-yl]methyl}cyclopropane-1-carboxamide (424 Da, Ligand)
- Anti-bril fab nanobody (15 kDa, Protein from synthetic construct)
- Anti-bril fab light chain (25 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Ccr6-bril/fab/nb in complex with sqa1 and oxm1 (120 MDa, Complex from Homo sapiens)
- 4-[[3,4-bis(oxidanylidene)-2-[[(1~{r})-1-(4-propan-2-ylfuran-2-yl)propyl]amino]cyclobuten-1-yl]amino]-~{n},~{n}-dimethyl-3-oxidanyl-pyridine-2-carboxamide (426 Da, Ligand)
- Anti-bril fab heavy chain (28 kDa, Protein from Homo sapiens)
Caspase and interleukin complex
Devant P, Dong Y, Mintseris J, Ma W, Gygi SP, Wu H, Kagan JC
Nature (2023) 624 pp. 451-459 [ Pubmed: 37993712 DOI: doi:10.1038/s41586-023-06751-9 ]
- Caspase in complex with interleukin (144 kDa, Complex from Homo sapiens)
Caspase-1 complex with interleukin-18
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8sv1
Q-score: 0.353
Dong Y, Bonin JP, Devant P, Liang Z, Sever AIM, Mintseris J, Aramini JM, Du G, Gygi SP, Kagan JC, Kay LE, Wu H
Immunity (2024) 57 pp. 1533-1548.e10 [ DOI: doi:10.1016/j.immuni.2024.04.015 Pubmed: 38733997 ]
- Interleukin-18 (21 kDa, Protein from Homo sapiens)
- Caspase-1 (16 kDa, Protein from Homo sapiens)
- Protein complex with interleukin (100 MDa, Complex from Homo sapiens)
- Caspase-1 (10 kDa, Protein from Homo sapiens)
Cryo-EM Structure of CdnG-E2 complex from Serratia marcescens (UltrAuFoil)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8hsb
Q-score: 0.38
Yan Y, Xiao J, Huang F, Xian W, Yu B, Cheng R, Wu H, Lu X, Wang X, Huang W, Li J, Oyejobi GK, Robinson CV, Wu H, Wu D, Liu X, Wang L, Zhu B
Nat Microbiol (2024) 9 pp. 1566-1578 [ DOI: doi:10.1038/s41564-024-01684-z Pubmed: 38649411 ]
- Cdng-e2 binary complex (Complex from Serratia marcescens)
- Type vi secretion protein (18 kDa, Protein from Serratia marcescens)
- Cdng (45 kDa, Protein from Serratia marcescens)
Cryo-EM Structure of CdnG-E2 complex from Serratia marcescens
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8yjy
Q-score: 0.426
Yan Y, Xiao J, Huang F, Xian W, Yu B, Cheng R, Wu H, Lu X, Wang X, Huang W, Li J, Oyejobi GK, Robinson CV, Wu H, Wu D, Liu X, Wang L, Zhu B
Nat Microbiol (2024) 9 pp. 1566-1578 [ DOI: doi:10.1038/s41564-024-01684-z Pubmed: 38649411 ]
- Type vi secretion protein (18 kDa, Protein from Serratia marcescens)
- Cdng-e2 binary complex (Complex from Serratia marcescens)
- Smcdng (45 kDa, Protein from Serratia marcescens)