Dong Y, Pi X, Bartels-Burgahn F, Saltukoglu D, Liang Z, Yang J, Alt FW, Reth M, Wu H
Nature (2022) 612 pp. 156-161 [ Pubmed: 36228656 DOI: doi:10.1038/s41586-022-05412-7 ]
- Bcr complex (Complex from Mus musculus)
Resolution: 8.2 Å
EM Method: Single-particle
Fitted PDBs: 8ema
Q-score: 0.031
Dong Y, Pi X, Bartels-Burgahn F, Saltukoglu D, Liang Z, Yang J, Alt FW, Reth M, Wu H
Nature (2022) 612 pp. 156-161 [ Pubmed: 36228656 DOI: doi:10.1038/s41586-022-05412-7 ]
- Bcr complex (Complex from Mus musculus)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 8e4c
Q-score: 0.232
Dong Y, Pi X, Bartels-Burgahn F, Saltukoglu D, Liang Z, Yang J, Alt FW, Reth M, Wu H
Nature (2022) 612 pp. 156-161 [ Pubmed: 36228656 DOI: doi:10.1038/s41586-022-05412-7 ]
- B-cell antigen receptor complex-associated protein beta chain (25 kDa, Protein from Mus musculus)
- Isoform 2 of immunoglobulin heavy constant mu (45 kDa, Protein from Mus musculus)
- B-cell antigen receptor complex-associated protein alpha chain,yellow fluorescent protein (42 kDa, Protein from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Bcr complex (Complex from Mus musculus)
Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS nicked DNA intermediates
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6dbi
Q-score: 0.324
Ru H, Mi W, Zhang P, Alt FW, Schatz DG, Liao M, Wu H
Nat Struct Mol Biol (2018) 25 pp. 732-742 [ DOI: doi:10.1038/s41594-018-0098-5 Pubmed: 30061602 ]
- Forward strand of coding flank (4 kDa, DNA from Danio rerio)
- Recombination activating gene 1 - mbp chimera (131 kDa, Protein from Danio rerio)
- Calcium ion (40 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Forward strand of 12-rss signal end (10 kDa, DNA from Danio rerio)
- Recombination activating gene 2 (59 kDa, Protein from Danio rerio)
- Rag in complex with 12-rss and 23-rss nicked dna intermediates (Complex from Danio rerio)
- Reverse strand of 12-rss (15 kDa, DNA from Danio rerio)
- Reverse strand of 23-rss (18 kDa, DNA from Danio rerio)
- Forward strand of 23-rss signal end (13 kDa, DNA from Danio rerio)
Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS nicked DNA intermediates
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6dbj
Q-score: 0.522
Ru H, Mi W, Zhang P, Alt FW, Schatz DG, Liao M, Wu H
Nat Struct Mol Biol (2018) 25 pp. 732-742 [ DOI: doi:10.1038/s41594-018-0098-5 Pubmed: 30061602 ]
- Forward strand of coding flank (4 kDa, DNA from Danio rerio)
- Recombination activating gene 1 - mbp chimera (131 kDa, Protein from Danio rerio)
- Zinc ion (65 Da, Ligand)
- Recombination activating gene 2 (59 kDa, Protein from Danio rerio)
- Reverse stand of rss (9 kDa, DNA from Danio rerio)
- Rag in complex with 12-rss and 23-rss nicked dna intermediates (Complex from Danio rerio)
- Calcium ion (40 Da, Ligand)
- Forward stand of rss signal end (4 kDa, DNA from Danio rerio)
Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs
Resolution: 5.0 Å
EM Method: Single-particle
Fitted PDBs: 6dbl
Q-score: 0.23
Ru H, Mi W, Zhang P, Alt FW, Schatz DG, Liao M, Wu H
Nat Struct Mol Biol (2018) 25 pp. 732-742 [ DOI: doi:10.1038/s41594-018-0098-5 Pubmed: 30061602 ]
- Rag in complex with 12-rss and 23-rss substrate dnas (Complex from Danio rerio)
- Molecule name: reverse strand of 12-rss substrate dna (15 kDa, DNA from Danio rerio)
- Molecule name: reverse strand of 23-rss substrate dna (18 kDa, DNA from Danio rerio)
- Recombination activating gene 1 - mbp chimera (131 kDa, Protein from Danio rerio)
- Zinc ion (65 Da, Ligand)
- Recombination activating gene 2 (59 kDa, Protein from Danio rerio)
- Calcium ion (40 Da, Ligand)
- Molecule name: forward strand of 12-rss substrate dna (15 kDa, DNA from Danio rerio)
- Molecule name: forward strand of 23-rss substrate dna (18 kDa, DNA from Danio rerio)
Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 6dbo
Q-score: 0.297
Ru H, Mi W, Zhang P, Alt FW, Schatz DG, Liao M, Wu H
Nat Struct Mol Biol (2018) 25 pp. 732-742 [ DOI: doi:10.1038/s41594-018-0098-5 Pubmed: 30061602 ]
- Rag in complex with 12-rss and 23-rss substrate dnas (Complex from Danio rerio)
- Recombination activating gene 1 - mbp chimera (131 kDa, Protein from Danio rerio)
- Reverse strand of substrate rss dna (9 kDa, DNA from Danio rerio)
- Zinc ion (65 Da, Ligand)
- Forward strand of substrate rss dna (9 kDa, DNA from Danio rerio)
- Recombination activating gene 2 (59 kDa, Protein from Danio rerio)
- Calcium ion (40 Da, Ligand)
Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs
Resolution: 4.22 Å
EM Method: Single-particle
Fitted PDBs: 6dbq
Q-score: 0.296
Ru H, Mi W, Zhang P, Alt FW, Schatz DG, Liao M, Wu H
Nat Struct Mol Biol (2018) 25 pp. 732-742 [ DOI: doi:10.1038/s41594-018-0098-5 Pubmed: 30061602 ]
- Rag in complex with 12-rss and 23-rss substrate dnas (Complex from Danio rerio)
- Molecule name: reverse strand of 12-rss substrate dna (15 kDa, DNA from Danio rerio)
- Reverse strand of 23-rss substrate dna (18 kDa, DNA from Danio rerio)
- Recombination activating gene 1 - mbp chimera (131 kDa, Protein from Danio rerio)
- Zinc ion (65 Da, Ligand)
- Recombination activating gene 2 (59 kDa, Protein from Danio rerio)
- Calcium ion (40 Da, Ligand)
- Molecule name: forward strand of 12-rss substrate dna (15 kDa, DNA from Danio rerio)
- Molecule name: forward strand of 23-rss substrate dna (18 kDa, DNA from Danio rerio)
Cryo-EM structure of RAG in complex with one melted RSS and one unmelted RSS
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6dbr
Q-score: 0.36
Ru H, Mi W, Zhang P, Alt FW, Schatz DG, Liao M, Wu H
Nat Struct Mol Biol (2018) 25 pp. 732-742 [ DOI: doi:10.1038/s41594-018-0098-5 Pubmed: 30061602 ]
- Reverse strand of unmelted rss substrate dna (10 kDa, DNA from Danio rerio)
- Forward strand of unmelted rss substrate dna (10 kDa, DNA from Danio rerio)
- Recombination activating gene 1 - mbp chimera (131 kDa, Protein from Danio rerio)
- Zinc ion (65 Da, Ligand)
- Recombination activating gene 2 (59 kDa, Protein from Danio rerio)
- Rag in complex with one melted rss and one unmelted rss (Complex from Danio rerio)
- Calcium ion (40 Da, Ligand)
- Reverse strand of melted rss substrate dna (10 kDa, DNA from Danio rerio)
- Forward strand of melted rss substrate dna (10 kDa, DNA from Danio rerio)
Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6dbt
Q-score: 0.323
Ru H, Mi W, Zhang P, Alt FW, Schatz DG, Liao M, Wu H
Nat Struct Mol Biol (2018) 25 pp. 732-742 [ DOI: doi:10.1038/s41594-018-0098-5 Pubmed: 30061602 ]
- Rag in complex with 12-rss and 23-rss substrate dnas (Complex from Danio rerio)
- Reverse strand of 23-rss substrate dna (18 kDa, DNA from Danio rerio)
- Recombination activating gene 1 - mbp chimera (131 kDa, Protein from Danio rerio)
- Reverse strand of 12-rss substrate dna (15 kDa, DNA from Danio rerio)
- Zinc ion (65 Da, Ligand)
- Recombination activating gene 2 (59 kDa, Protein from Danio rerio)
- Calcium ion (40 Da, Ligand)
- Forward strand of 23-rss substrate dna (18 kDa, DNA from Danio rerio)
- Forward strand of 12-rss substrate dna (15 kDa, DNA from Danio rerio)