Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 6dbo
Q-score: 0.297
Ru H, Mi W, Zhang P, Alt FW, Schatz DG, Liao M, Wu H
Nat Struct Mol Biol (2018) 25 pp. 732-742 [ DOI: doi:10.1038/s41594-018-0098-5 Pubmed: 30061602 ]
- Rag in complex with 12-rss and 23-rss substrate dnas (Complex from Danio rerio)
- Recombination activating gene 1 - mbp chimera (131 kDa, Protein from Danio rerio)
- Reverse strand of substrate rss dna (9 kDa, DNA from Danio rerio)
- Zinc ion (65 Da, Ligand)
- Forward strand of substrate rss dna (9 kDa, DNA from Danio rerio)
- Recombination activating gene 2 (59 kDa, Protein from Danio rerio)
- Calcium ion (40 Da, Ligand)
Cryo-EM structure of RAG in complex with 12-RSS and 23-RSS substrate DNAs
Resolution: 4.22 Å
EM Method: Single-particle
Fitted PDBs: 6dbq
Q-score: 0.296
Ru H, Mi W, Zhang P, Alt FW, Schatz DG, Liao M, Wu H
Nat Struct Mol Biol (2018) 25 pp. 732-742 [ DOI: doi:10.1038/s41594-018-0098-5 Pubmed: 30061602 ]
- Rag in complex with 12-rss and 23-rss substrate dnas (Complex from Danio rerio)
- Molecule name: reverse strand of 12-rss substrate dna (15 kDa, DNA from Danio rerio)
- Reverse strand of 23-rss substrate dna (18 kDa, DNA from Danio rerio)
- Recombination activating gene 1 - mbp chimera (131 kDa, Protein from Danio rerio)
- Zinc ion (65 Da, Ligand)
- Recombination activating gene 2 (59 kDa, Protein from Danio rerio)
- Calcium ion (40 Da, Ligand)
- Molecule name: forward strand of 12-rss substrate dna (15 kDa, DNA from Danio rerio)
- Molecule name: forward strand of 23-rss substrate dna (18 kDa, DNA from Danio rerio)
Cryo-EM structure of RAG in complex with one melted RSS and one unmelted RSS
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6dbr
Q-score: 0.36
Ru H, Mi W, Zhang P, Alt FW, Schatz DG, Liao M, Wu H
Nat Struct Mol Biol (2018) 25 pp. 732-742 [ DOI: doi:10.1038/s41594-018-0098-5 Pubmed: 30061602 ]
- Reverse strand of unmelted rss substrate dna (10 kDa, DNA from Danio rerio)
- Forward strand of unmelted rss substrate dna (10 kDa, DNA from Danio rerio)
- Recombination activating gene 1 - mbp chimera (131 kDa, Protein from Danio rerio)
- Zinc ion (65 Da, Ligand)
- Recombination activating gene 2 (59 kDa, Protein from Danio rerio)
- Rag in complex with one melted rss and one unmelted rss (Complex from Danio rerio)
- Calcium ion (40 Da, Ligand)
- Reverse strand of melted rss substrate dna (10 kDa, DNA from Danio rerio)
- Forward strand of melted rss substrate dna (10 kDa, DNA from Danio rerio)
Cryo-EM structure of RAG in complex with melted 12-RSS and unmelted 23-RSS substrates
Resolution: 4.29 Å
EM Method: Single-particle
Fitted PDBs: 6dbv
Q-score: 0.296
Ru H, Mi W, Zhang P, Alt FW, Schatz DG, Liao M, Wu H
Nat Struct Mol Biol (2018) 25 pp. 732-742 [ DOI: doi:10.1038/s41594-018-0098-5 Pubmed: 30061602 ]
- Rag in complex with 12-rss and 23-rss substrate dnas (Complex from Danio rerio)
- Reverse strand of 23-rss substrate dna (18 kDa, DNA from Danio rerio)
- Recombination activating gene 1 - mbp chimera (131 kDa, Protein from Danio rerio)
- Reverse strand of 12-rss substrate dna (15 kDa, DNA from Danio rerio)
- Zinc ion (65 Da, Ligand)
- Recombination activating gene 2 (59 kDa, Protein from Danio rerio)
- Calcium ion (40 Da, Ligand)
- Forward strand of 23-rss substrate dna (18 kDa, DNA from Danio rerio)
- Forward strand of 12-rss substrate dna (15 kDa, DNA from Danio rerio)
- Recombination activating gene 1 - mbp chimera (131 kDa, Protein from Danio rerio)
Cryo-electron microscopy structure of RAG SEC (C2 symmetry)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 3jbx
Q-score: 0.447
Ru H, Chambers MG, Fu TM, Tong AB, Liao M, Wu H
Cell (2015) 163 pp. 1138-1152 [ Pubmed: 26548953 DOI: doi:10.1016/j.cell.2015.10.055 ]
- Signal end complex of rag1-rag2 (380 kDa, Sample)
- Recombination activating gene 1 and 2 (380 kDa, Protein from Danio rerio)
Cryo-electron microscopy structure of RAG PC (C2 symmetry)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 3jby
Q-score: 0.435
Ru H, Chambers MG, Fu TM, Tong AB, Liao M, Wu H
Cell (2015) 163 pp. 1138-1152 [ Pubmed: 26548953 DOI: doi:10.1016/j.cell.2015.10.055 ]
- Recombination activating gene 1 and 2 (400 kDa, Protein from Danio rerio)
- Paired complex of rag1-rag2 (400 kDa, Sample)
Cryo-electron microscopy structure of RAG PC (with NBD, no symmetry)
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 3jbw
Q-score: 0.245
Ru H, Chambers MG, Fu TM, Tong AB, Liao M, Wu H
Cell (2015) 163 pp. 1138-1152 [ Pubmed: 26548953 DOI: doi:10.1016/j.cell.2015.10.055 ]
- Recombination activating gene 1 and 2 (400 kDa, Protein from Danio rerio)
- Paired complex of rag1-rag2 (400 kDa, Sample)
Cryo-electron microscopy structure from the combined data of RAG SEC and PC (C2 symmetry)
Ru H, Chambers MG, Fu TM, Tong AB, Liao M, Wu H
Cell (2015) 163 pp. 1138-1152 [ Pubmed: 26548953 DOI: doi:10.1016/j.cell.2015.10.055 ]
- Recombination activating gene 1 and 2 (400 kDa, Protein from Danio rerio)
- Combined rag sec and pc (400 kDa, Sample)