An Agonist and PAM-bound Class A GPCR with Gi protein complex structure
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7v68
Q-score: 0.334
Wang J, Wu M, Chen Z, Wu L, Wang T, Cao D, Wang H, Liu S, Xu Y, Li F, Liu J, Chen N, Zhao S, Cheng J, Wang S, Hua T
Nat Commun (2022) 13 pp. 2855-2855 [ DOI: doi:10.1038/s41467-022-30595-y Pubmed: 35606397 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Homo sapiens)
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
- Gi complex (Complex from Homo sapiens)
- M4r_iperoxo_ly2119620_gi complex (Complex from Homo sapiens)
- Muscarinic acetylcholine receptor m4r (Complex)
- 3-amino-5-chloro-n-cyclopropyl-4-methyl-6-[2-(4-methylpiperazin-1-yl)-2-oxoethoxy]thieno[2,3-b]pyridine-2-carboxamide (437 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- 4-(4,5-dihydro-1,2-oxazol-3-yloxy)-n,n,n-trimethylbut-2-yn-1-aminium (197 Da, Ligand)
- Scfv16 (27 kDa, Protein from Homo sapiens)
Cryo-EM structure of a class A GPCR-G protein complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7v69
Q-score: 0.324
Wang J, Wu M, Chen Z, Wu L, Wang T, Cao D, Wang H, Liu S, Xu Y, Li F, Liu J, Chen N, Zhao S, Cheng J, Wang S, Hua T
Nat Commun (2022) 13 pp. 2855-2855 [ DOI: doi:10.1038/s41467-022-30595-y Pubmed: 35606397 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- M4r-iperoxo and gi protein complex (Complex from Homo sapiens)
- Gi protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Homo sapiens)
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- M4r-iperoxo (Complex)
- Scfv16 (27 kDa, Protein from Homo sapiens)
Cry-EM structure of M4-c110-G protein complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7v6a
Q-score: 0.37
Wang J, Wu M, Chen Z, Wu L, Wang T, Cao D, Wang H, Liu S, Xu Y, Li F, Liu J, Chen N, Zhao S, Cheng J, Wang S, Hua T
Nat Commun (2022) 13 pp. 2855-2855 [ DOI: doi:10.1038/s41467-022-30595-y Pubmed: 35606397 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- M4r-compound110-gi complex (Complex from Homo sapiens)
- Scfv16 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
- Gi complex (Complex from Homo sapiens)
- Methyl 4-[4-(3-methyl-2-oxidanylidene-benzimidazol-1-yl)piperidin-1-yl]piperidine-1-carboxylate (372 Da, Ligand)
- Muscarinic acetylcholine receptor m4r (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Homo sapiens)
Cryo-EM structure of a class A GPCR
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6lfm
Q-score: 0.461
Liu K, Wu L, Yuan S, Wu M, Xu Y, Sun Q, Li S, Zhao S, Hua T, Liu ZJ
Nature (2020) 585 pp. 135-140 [ Pubmed: 32610344 DOI: doi:10.1038/s41586-020-2492-5 ]
- Cholesterol (386 Da, Ligand)
- Interleukin-8 (8 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Ternary complex of chemokine, gpcr and g protein (150 kDa, Complex from Homo sapiens)
- C-x-c chemokine receptor type 2 (40 kDa, Protein from Homo sapiens)
Cryo-EM structure of a class A GPCR monomer
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6lfo
Q-score: 0.441
Liu K, Wu L, Yuan S, Wu M, Xu Y, Sun Q, Li S, Zhao S, Hua T, Liu ZJ
Nature (2020) 585 pp. 135-140 [ DOI: doi:10.1038/s41586-020-2492-5 Pubmed: 32610344 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Interleukin-8 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- C-x-c chemokine receptor type 2 (40 kDa, Protein from Homo sapiens)
- Ternary complex of chemokine-monomer, gpcr and g protein (150 kDa, Complex from Homo sapiens)
- Scfv16 (27 kDa, Protein from Homo sapiens)
Cryo-EM structure of a class A GPCR with G protein complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6kpf
Q-score: 0.556
Hua T, Li X, Wu L, Iliopoulos-Tsoutsouvas C, Wang Y, Wu M, Shen L, Johnston CA, Nikas SP, Song F, Song X, Yuan S, Sun Q, Wu Y, Jiang S, Grim TW, Benchama O, Stahl EL, Zvonok N, Zhao S, Bohn LM, Makriyannis A, Liu ZJ
Cell (2020) 180 pp. 655- [ DOI: doi:10.1016/j.cell.2020.01.008 Pubmed: 32004463 ]
- Scfv16 (27 kDa, Protein from Homo sapiens)
- Cannabinoid receptor 2 (39 kDa, Protein from Homo sapiens)
- 7-[(6ar,9r,10ar)-1-hydroxy-9-(hydroxymethyl)-6,6-dimethyl-6a,7,8,9,10,10a-hexahydro-6h-benzo[c]chromen-3-yl]- 7-methyloctanenitrile (399 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Gpcr-g protein complex (130 MDa, Complex from Homo sapiens)
Cryo-EM structure of CB1-G protein complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6kpg
Q-score: 0.501
Hua T, Li X, Wu L, Iliopoulos-Tsoutsouvas C, Wang Y, Wu M, Shen L, Johnston CA, Nikas SP, Song F, Song X, Yuan S, Sun Q, Wu Y, Jiang S, Grim TW, Benchama O, Stahl EL, Zvonok N, Zhao S, Bohn LM, Makriyannis A, Liu ZJ
Cell (2020) 180 pp. 655- [ DOI: doi:10.1016/j.cell.2020.01.008 Pubmed: 32004463 ]
- Scfv16 (27 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Cb1-g protein complex (140 MDa, Complex from Homo sapiens)
- Cannabinoid receptor 1 (52 kDa, Protein from Homo sapiens)
- (6~{a}~{r},9~{r},10~{a}~{r})-9-(hydroxymethyl)-3-(8-isothiocyanato-2-methyl-octan-2-yl)-6,6-dimethyl-6~{a},7,8,9,10,10~{a}-hexahydrobenzo[c]chromen-1-ol (445 Da, Ligand)