Cryo-EM structure of a class A orphan GPCR in complex with Gi
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7vug
Q-score: 0.445
Zhou Y, Daver H, Trapkov B, Wu L, Wu M, Harpsoe K, Gentry PR, Liu K, Larionova M, Liu J, Chen N, Brauner-Osborne H, Gloriam DE, Hua T, Liu ZJ
Cell Res (2022) 32 pp. 210-213 [ DOI: doi:10.1038/s41422-021-00591-w Pubmed: 34916631 ]
- 3-chloranyl-n-[2-oxidanylidene-2-[[(1s)-1-phenylethyl]amino]ethyl]benzamide (316 Da, Ligand)
- Ternary complex of ligand, gpcr and g protein (180 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Bacillus circulans)
- Endo-1,4-beta-xylanase,probable g-protein coupled receptor 139 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Chimera of endo-1,4-beta-xylanase and probable g-protein coupled receptor 139 (60 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (Complex from Homo sapiens)
- Scfv16 (Complex)
- Scfv16 (27 kDa, Protein from Homo sapiens)
Cryo-EM structure of a class A orphan GPCR
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 7vuh
Q-score: 0.409
Zhou Y, Daver H, Trapkov B, Wu L, Wu M, Harpsoe K, Gentry PR, Liu K, Larionova M, Liu J, Chen N, Brauner-Osborne H, Gloriam DE, Hua T, Liu ZJ
Cell Res (2022) 32 pp. 210-213 [ DOI: doi:10.1038/s41422-021-00591-w Pubmed: 34916631 ]
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Nanobody 35 (Complex from Bacillus circulans)
- Chimera of endo-1,4-beta-xylanase and probable g-protein coupled receptor 139 (60 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- 3-chloranyl-n-[2-oxidanylidene-2-[[(1s)-1-phenylethyl]amino]ethyl]benzamide (316 Da, Ligand)
- Nanobody 35 (17 kDa, Protein from Homo sapiens)
- Endo-1,4-beta-xylanase,probable g-protein coupled receptor 139 (Complex)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
- Ternary complex of ligand, gpcr and g protein (150 kDa, Complex from Homo sapiens)
Cryo-EM structure of a class A orphan GPCR
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7vui
Q-score: 0.389
Zhou Y, Daver H, Trapkov B, Wu L, Wu M, Harpsoe K, Gentry PR, Liu K, Larionova M, Liu J, Chen N, Brauner-Osborne H, Gloriam DE, Hua T, Liu ZJ
Cell Res (2022) 32 pp. 210-213 [ DOI: doi:10.1038/s41422-021-00591-w Pubmed: 34916631 ]
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Nanobody 35 (Complex from Bacillus circulans)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
- Chimera of endo-1,4-beta-xylanase and probable g-protein coupled receptor 139 (60 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- 3-chloranyl-n-[2-oxidanylidene-2-[[(1s)-1-phenylethyl]amino]ethyl]benzamide (316 Da, Ligand)
- Nanobody 35 (17 kDa, Protein from Homo sapiens)
- Endo-1,4-beta-xylanase,probable g-protein coupled receptor 139 (Complex)
- Ternary complex of ligand, gpcr and g protein (150 kDa, Complex from Homo sapiens)
Cryo-EM structure of a class A orphan GPCR
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7vuj
Q-score: 0.212
Zhou Y, Daver H, Trapkov B, Wu L, Wu M, Harpsoe K, Gentry PR, Liu K, Larionova M, Liu J, Chen N, Brauner-Osborne H, Gloriam DE, Hua T, Liu ZJ
Cell Res (2022) 32 pp. 210-213 [ DOI: doi:10.1038/s41422-021-00591-w Pubmed: 34916631 ]
- Chimera endo-1,4-beta-xylanase and probable g-protein coupled receptor 139 (Complex)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Nanobody 35 (Complex from Bacillus circulans)
- Chimera of endo-1,4-beta-xylanase and probable g-protein coupled receptor 139 (60 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
- 3-chloranyl-n-[2-oxidanylidene-2-[[(1s)-1-phenylethyl]amino]ethyl]benzamide (316 Da, Ligand)
- Nanobody 35 (17 kDa, Protein from Homo sapiens)
- Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
- Ternary complex of ligand, gpcr and g protein (150 kDa, Complex from Homo sapiens)
Cryo-EM structure of a class A GPCR
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6lfm
Q-score: 0.461
Liu K, Wu L, Yuan S, Wu M, Xu Y, Sun Q, Li S, Zhao S, Hua T, Liu ZJ
Nature (2020) 585 pp. 135-140 [ Pubmed: 32610344 DOI: doi:10.1038/s41586-020-2492-5 ]
- Cholesterol (386 Da, Ligand)
- Interleukin-8 (8 kDa, Protein from Homo sapiens)
- Scfv16 (27 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Ternary complex of chemokine, gpcr and g protein (150 kDa, Complex from Homo sapiens)
- C-x-c chemokine receptor type 2 (40 kDa, Protein from Homo sapiens)
Cryo-EM structure of a class A GPCR monomer
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6lfo
Q-score: 0.441
Liu K, Wu L, Yuan S, Wu M, Xu Y, Sun Q, Li S, Zhao S, Hua T, Liu ZJ
Nature (2020) 585 pp. 135-140 [ DOI: doi:10.1038/s41586-020-2492-5 Pubmed: 32610344 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Interleukin-8 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- C-x-c chemokine receptor type 2 (40 kDa, Protein from Homo sapiens)
- Ternary complex of chemokine-monomer, gpcr and g protein (150 kDa, Complex from Homo sapiens)
- Scfv16 (27 kDa, Protein from Homo sapiens)
Cryo-EM structure of a class A GPCR with G protein complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6kpf
Q-score: 0.556
Hua T, Li X, Wu L, Iliopoulos-Tsoutsouvas C, Wang Y, Wu M, Shen L, Johnston CA, Nikas SP, Song F, Song X, Yuan S, Sun Q, Wu Y, Jiang S, Grim TW, Benchama O, Stahl EL, Zvonok N, Zhao S, Bohn LM, Makriyannis A, Liu ZJ
Cell (2020) 180 pp. 655- [ DOI: doi:10.1016/j.cell.2020.01.008 Pubmed: 32004463 ]
- Scfv16 (27 kDa, Protein from Homo sapiens)
- Cannabinoid receptor 2 (39 kDa, Protein from Homo sapiens)
- 7-[(6ar,9r,10ar)-1-hydroxy-9-(hydroxymethyl)-6,6-dimethyl-6a,7,8,9,10,10a-hexahydro-6h-benzo[c]chromen-3-yl]- 7-methyloctanenitrile (399 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Gpcr-g protein complex (130 MDa, Complex from Homo sapiens)
Cryo-EM structure of CB1-G protein complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6kpg
Q-score: 0.501
Hua T, Li X, Wu L, Iliopoulos-Tsoutsouvas C, Wang Y, Wu M, Shen L, Johnston CA, Nikas SP, Song F, Song X, Yuan S, Sun Q, Wu Y, Jiang S, Grim TW, Benchama O, Stahl EL, Zvonok N, Zhao S, Bohn LM, Makriyannis A, Liu ZJ
Cell (2020) 180 pp. 655- [ DOI: doi:10.1016/j.cell.2020.01.008 Pubmed: 32004463 ]
- Scfv16 (27 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Cb1-g protein complex (140 MDa, Complex from Homo sapiens)
- Cannabinoid receptor 1 (52 kDa, Protein from Homo sapiens)
- (6~{a}~{r},9~{r},10~{a}~{r})-9-(hydroxymethyl)-3-(8-isothiocyanato-2-methyl-octan-2-yl)-6,6-dimethyl-6~{a},7,8,9,10,10~{a}-hexahydrobenzo[c]chromen-1-ol (445 Da, Ligand)