Anti-CRISPR protein AcrF1/2 bound to Csy surveillance complex with a 20nt spacer crRNA
Peng R, Xu Y, Zhu T, Li N, Qi J, Chai Y, Wu M, Zhang X, Shi Y, Wang P, Wang J, Gao N, Gao GF
Cell Res (2017) 27 pp. 853-864 [ DOI: doi:10.1038/cr.2017.79 Pubmed: 28574055 ]
- Csy2 (Protein from Pseudomonas aeruginosa)
- Anti-crispr proteins acrf1/2 bound to csy surveillance complex with a 20nt spacer crrna (300 kDa, Complex from Pseudomonas aeruginosa)
- Csy1 (Protein from Pseudomonas aeruginosa)
- Csy4 (Protein from Pseudomonas aeruginosa)
- Csy3 (Protein from Pseudomonas aeruginosa)
- Acrf1 (Protein from Pseudomonas phage D3)
- Crrna with 20nt spacer (RNA from Pseudomonas aeruginosa)
- Acrf2 (Protein from Pseudomonas phage 73)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 5xlo
Q-score: 0.337
Peng R, Xu Y, Zhu T, Li N, Qi J, Chai Y, Wu M, Zhang X, Shi Y, Wang P, Wang J, Gao N, Gao GF
Cell Res (2017) 27 pp. 853-864 [ DOI: doi:10.1038/cr.2017.79 Pubmed: 28574055 ]
- Uncharacterized protein acrf1 (8 kDa, Protein from Pseudomonas phage JBD30)
- Anti-crispr proteins acrf1/2 bound to csy surveillance complex with a 32nt spacer crrna backbone region (400 kDa, Complex from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Crrna with 32nt spacer sequence (19 kDa, RNA from Pseudomonas aeruginosa)
- Crispr-associated protein csy3 (Complex)
- Acrf1 (Complex)
- Crrna with 20nt spacer sequence (Complex)
- Crispr-associated protein csy3 (37 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
Anti-CRISPR protein AcrF1 bound to Csy surveillance complex with 32nt spacer crRNA in binding mode B
Peng R, Xu Y, Zhu T, Li N, Qi J, Chai Y, Wu M, Zhang X, Shi Y, Wang P, Wang J, Gao N, Gao GF
Cell Res (2017) 27 pp. 853-864 [ DOI: doi:10.1038/cr.2017.79 Pubmed: 28574055 ]
- Crrna with 32nt spacer (RNA from Pseudomonas aeruginosa)
- Csy2 (Protein from Pseudomonas aeruginosa)
- Csy1 (Protein from Pseudomonas aeruginosa)
- Csy4 (Protein from Pseudomonas aeruginosa)
- Csy3 (Protein from Pseudomonas aeruginosa)
- Acrf1 (Protein from Pseudomonas aeruginosa)
- Anti-crispr protein acrf1 bound to csy surveillance complex with 32nt spacer crrna in binding mode b (400 kDa, Complex from Pseudomonas aeruginosa)
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 5xlp
Q-score: 0.26
Peng R, Xu Y, Zhu T, Li N, Qi J, Chai Y, Wu M, Zhang X, Shi Y, Wang P, Wang J, Gao N, Gao GF
Cell Res (2017) 27 pp. 853-864 [ DOI: doi:10.1038/cr.2017.79 Pubmed: 28574055 ]
- Crrna with 20nt spacer sequence (15 kDa, RNA from Pseudomonas aeruginosa)
- Crispr-associated protein csy3 (37 kDa, Protein from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Anti-crispr proteins acrf1/2 bound to csy surveillance complex with a 20nt spacer sequence backbone region (300 kDa, Complex from Pseudomonas aeruginosa (strain UCBPP-PA14))
- Uncharacterized protein acrf1 (8 kDa, Protein from Pseudomonas phage JBD30)
- Man crispr-associated protein csy3 (Complex)
- Rna (Complex)
- Acrf1 (Complex)