The cryo-EM structure of C. crescentus GcrA-TACup
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7ye1
Q-score: 0.407
Nucleic Acids Res (2023) [ ]
- Magnesium ion (24 Da, Ligand)
- Dna (57-mer)-non template (17 kDa, DNA from Caulobacter vibrioides NA1000)
- Dna-directed rna polymerase subunit beta (151 kDa, Protein from Caulobacter vibrioides NA1000)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Caulobacter vibrioides NA1000)
- Dna-directed rna polymerase subunit beta' (154 kDa, Protein from Caulobacter vibrioides NA1000)
- Cell cycle regulatory protein gcra (18 kDa, Protein from Caulobacter vibrioides NA1000)
- Dna (57-mer)-template (17 kDa, DNA from Caulobacter vibrioides NA1000)
- Dna-directed rna polymerase/gcra/tacup (Complex)
- Zinc ion (65 Da, Ligand)
- Dna (Complex)
- Rna polymerase sigma factor rpod (72 kDa, Protein from Caulobacter vibrioides NA1000)
- The cryo-em structure of c. crescentus gcra-tacup (Complex from Caulobacter vibrioides NA1000)
- Dna-directed rna polymerase subunit omega (13 kDa, Protein from Caulobacter vibrioides NA1000)
The cryo-EM structure of C. crescentus GcrA-TACdown
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7ye2
Q-score: 0.354
Nucleic Acids Res (2023) [ ]
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta (151 kDa, Protein from Caulobacter vibrioides NA1000)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Caulobacter vibrioides NA1000)
- Dna-directed rna polymerase subunit beta' (154 kDa, Protein from Caulobacter vibrioides NA1000)
- Cell cycle regulatory protein gcra (18 kDa, Protein from Caulobacter vibrioides NA1000)
- Zinc ion (65 Da, Ligand)
- Rna polymerase sigma factor rpod (72 kDa, Protein from Caulobacter vibrioides NA1000)
- Dna-directed rna polymerase/rpod/gcra (Complex)
- Dna (90-mer)-template (27 kDa, DNA from Caulobacter vibrioides NA1000)
- Dna (90-mer)-non template (27 kDa, DNA from Caulobacter vibrioides NA1000)
- Dna (Complex)
- The cryo-em structure of c. crescentus gcra-tacdown (Complex from Caulobacter vibrioides NA1000)
- Dna-directed rna polymerase subunit omega (13 kDa, Protein from Caulobacter vibrioides NA1000)
The cryo-EM structure of A. thaliana Pol IV-RDR2 backtracked complex
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 7eu0
Q-score: 0.487
Huang K, Wu XX, Fang CL, Xu ZG, Zhang HW, Gao J, Zhou CM, You LL, Gu ZX, Mu WH, Feng Y, Wang JW, Zhang Y
Science (2021) 374 pp. 1579-1586 [ Pubmed: 34941388 DOI: doi:10.1126/science.abj9184 ]
- Dna-directed rna polymerases ii, iv and v subunit 12 (5 kDa, Protein from Arabidopsis thaliana)
- Dna (5'-d(*cp*tp*gp*tp*gp*cp*tp*tp*ap*tp*cp*gp*gp*tp*ap*gp*ap*gp*tp*g)-3') (6 kDa, DNA from synthetic construct)
- Dna-directed rna polymerases ii, iv and v subunit 10 (8 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 3 (35 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases iv and v subunit 2 (132 kDa, Protein from Arabidopsis thaliana)
- Magnesium ion (24 Da, Ligand)
- Rna (39-mer) (12 kDa, RNA from synthetic construct)
- Dna-directed rna polymerases ii and iv subunit 5a (24 kDa, Protein from Arabidopsis thaliana)
- Rna-dependent rna polymerase 2 (129 kDa, Protein from Arabidopsis thaliana)
- Rna (5'-r(*cp*cp*gp*a)-3') (1 kDa, RNA from synthetic construct)
- A. thaliana pol iv-rdr2 backtracked complex (550 kDa, Complex from Arabidopsis thaliana)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerases ii, iv and v subunit 11 (13 kDa, Protein from Arabidopsis thaliana)
- Dna (33-mer) (9 kDa, DNA from synthetic construct)
- Dna-directed rna polymerases ii, iv and v subunit 9a (13 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 8b (16 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerase iv subunit 1 (169 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 6a (16 kDa, Protein from Arabidopsis thaliana)
The cryo-EM structure of A. thaliana Pol IV-RDR2 holoenzyme
Resolution: 3.86 Å
EM Method: Single-particle
Fitted PDBs: 7eu1
Q-score: 0.434
Huang K, Wu XX, Fang CL, Xu ZG, Zhang HW, Gao J, Zhou CM, You LL, Gu ZX, Mu WH, Feng Y, Wang JW, Zhang Y
Science (2021) 374 pp. 1579-1586 [ Pubmed: 34941388 DOI: doi:10.1126/science.abj9184 ]
- Dna-directed rna polymerases ii, iv and v subunit 12 (5 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 10 (8 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 3 (35 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases iv and v subunit 2 (132 kDa, Protein from Arabidopsis thaliana)
- A. thaliana pol iv-rdr2 holoenzyme (Complex from Arabidopsis thaliana)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases ii and iv subunit 5a (24 kDa, Protein from Arabidopsis thaliana)
- Rna-dependent rna polymerase 2 (129 kDa, Protein from Arabidopsis thaliana)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerases ii, iv and v subunit 11 (13 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 9a (13 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 8b (16 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerase iv subunit 1 (169 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 6a (16 kDa, Protein from Arabidopsis thaliana)