cryo-EM structure of Scap/Insig complex in the present of 25-hydroxyl cholesterol.
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6m49
Q-score: 0.335
Yan R, Cao P, Song W, Qian H, Du X, Coates HW, Zhao X, Li Y, Gao S, Gong X, Liu X, Sui J, Lei J, Yang H, Brown AJ, Zhou Q, Yan C, Yan N
Science (2021) 371 [ Pubmed: 33446483 DOI: doi:10.1126/science.abb2224 ]
- 25-hydroxycholesterol (402 Da, Ligand)
- Scap and insig complex (Complex from Homo sapiens)
- Sterol regulatory element-binding protein cleavage-activating protein,sterol regulatory element-binding protein cleavage-activating protein (63 kDa, Protein from Homo sapiens)
- Insulin-induced gene 2 protein (24 kDa, Protein from Homo sapiens)
Cryo-EM structure of tetrameric Ptch1 in complex with ShhNp (form I)
Resolution: 6.8 Å
EM Method: Single-particle
Fitted PDBs: 6n7g
Q-score: 0.132
Qian H, Cao P, Hu M, Gao S, Yan N, Gong X
Nat Commun (2019) 10 pp. 2320-2320 [ DOI: doi:10.1038/s41467-019-10234-9 Pubmed: 31127104 ]
- Zinc ion (65 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Patch1 (Cellular component from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Protein patched homolog 1 (150 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Sonic hedgehog protein (19 kDa, Protein from Homo sapiens)
Cryo-EM structure of the 2:1 hPtch1-Shhp complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6n7h
Q-score: 0.408
Qian H, Cao P, Hu M, Gao S, Yan N, Gong X
Nat Commun (2019) 10 pp. 2320-2320 [ DOI: doi:10.1038/s41467-019-10234-9 Pubmed: 31127104 ]
- Zinc ion (65 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Patch1 (Cellular component from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Protein patched homolog 1 (150 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Sonic hedgehog protein (19 kDa, Protein from Homo sapiens)
Cryo-EM structure of tetrameric Ptch1 in complex with ShhNp (form II)
Resolution: 6.5 Å
EM Method: Single-particle
Fitted PDBs: 6n7k
Q-score: 0.132
Qian H, Cao P, Hu M, Gao S, Yan N, Gong X
Nat Commun (2019) 10 pp. 2320-2320 [ DOI: doi:10.1038/s41467-019-10234-9 Pubmed: 31127104 ]
- Zinc ion (65 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Patch1 (Cellular component from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Protein patched homolog 1 (150 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Sonic hedgehog protein (19 kDa, Protein from Homo sapiens)
Cryo-EM structure of human Ptch1
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6dmb
Q-score: 0.408
Gong X, Qian H, Cao P, Zhao X, Zhou Q, Lei J, Yan N
Science (2018) 361 [ Pubmed: 29954986 DOI: doi:10.1126/science.aas8935 ]
- Cholesterol hemisuccinate (486 Da, Ligand)
- Protein patched homolog 1 (150 kDa, Protein from Homo sapiens)
- Patch1 (Cellular component from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Cryo-EM structure of human Ptch1 with three mutations L282Q/T500F/P504L
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6dmo
Q-score: 0.279
Gong X, Qian H, Cao P, Zhao X, Zhou Q, Lei J, Yan N
Science (2018) 361 [ Pubmed: 29954986 DOI: doi:10.1126/science.aas8935 ]
- Patch1 with l282q/t500f/p504l mutations (Cellular component from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Protein patched homolog 1 (150 kDa, Protein from Homo sapiens)
Cryo-EM structure of human Ptch1 and ShhN complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6dmy
Q-score: 0.388
Gong X, Qian H, Cao P, Zhao X, Zhou Q, Lei J, Yan N
Science (2018) 361 [ Pubmed: 29954986 DOI: doi:10.1126/science.aas8935 ]
- Serine (105 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Patch1 (Cellular component from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Sonic hedgehog protein (19 kDa, Protein from Homo sapiens)
- Protein patched homolog 1 (150 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
Cryo-EM structure of human ABCA1
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 5xjy
Q-score: 0.387
Qian H, Zhao X, Cao P, Lei J, Yan N, Gong X
Cell (2017) 169 pp. 1228-1239.e10 [ DOI: doi:10.1016/j.cell.2017.05.020 Pubmed: 28602350 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Membrane protein (Cellular component from Homo sapiens)
- Atp-binding cassette sub-family a member 1 (259 kDa, Protein from Homo sapiens)
cryo-EM map of the full-length human NPC1 in complex with the cleaved glycoprotein of Ebola virus
Resolution: 6.56 Å
EM Method: Single-particle
Fitted PDBs: 5jnx
Q-score: 0.14
Gong X, Qian H, Zhou X, Wu J, Wan T, Cao P, Huang W, Zhao X, Wang X, Wang P, Shi Y, Gao GF, Zhou Q, Yan N
Cell (2016) 165 pp. 1467-1478 [ DOI: doi:10.1016/j.cell.2016.05.022 Pubmed: 27238017 ]
- Npc1 in complex with ebov-gpcl trimer (300 kDa, Complex from Homo sapiens)
- Npc1 in complex with ebov-gpcl trimer (Protein from Homo sapiens)
cryo-EM map of the full-length human NPC1 at 4.4 angstrom
Resolution: 4.43 Å
EM Method: Single-particle
Fitted PDBs: 3jd8
Q-score: 0.224