Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7xve
Q-score: 0.526
Huang G, Wu Q, Li Z, Jin X, Huang X, Wu T, Pan X, Yan N
PNAS (2022) 119 pp. e2209164119-e2209164119 [ Pubmed: 35878056 DOI: doi:10.1073/pnas.2209164119 ]
- Ternary complex of alpha1/beta1/beta2 (300 kDa, Complex from Homo sapiens)
- Sodium channel subunit beta-1 (24 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- (2s,3r,4e)-2-(acetylamino)-3-hydroxyoctadec-4-en-1-yl dihydrogen phosphate (421 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Sodium channel protein type 9 subunit alpha (230 kDa, Protein from Homo sapiens)
- Sodium channel subunit beta-2 (24 kDa, Protein from Homo sapiens)
- 1-o-octadecyl-sn-glycero-3-phosphocholine (510 Da, Ligand)
- O-[(r)-{[(2r)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-l-serine (792 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
Cryo-EM structure of Scap/Insig complex in the present of digitonin.
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7etw
Q-score: 0.351
Yan R, Cao P, Song W, Li Y, Wang T, Qian H, Yan C, Yan N
Cell Rep (2021) 35 pp. 109299- [ DOI: doi:10.1016/j.celrep.2021.109299 ]
- Sterol regulatory element-binding protein cleavage-activating protein (81 kDa, Protein from Homo sapiens)
- Digitonin (1 kDa, Ligand)
- Insulin-induced gene 2 protein (24 kDa, Protein from Homo sapiens)
- Scap and insig complex (Complex from Homo sapiens)
3.9 Angstrom reconstruction of E.coli AcrB embedded in the liposome
PNAS (2020) 117 pp. 18497-18503 [ Pubmed: 32680969 DOI: doi:10.1073/pnas.2009385117 ]
- E.coli acrb (350 MDa, Complex from Escherichia coli)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6w5r
Q-score: 0.417
Qian H, Wu X, Du X, Yao X, Zhao X, Lee J, Yang H, Yan N
Cell (2020) 182 pp. 98-111.e18 [ DOI: doi:10.1016/j.cell.2020.05.020 Pubmed: 32544384 ]
- Npc1 nanodisc (150 kDa, Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Npc intracellular cholesterol transporter 1 (146 kDa, Protein from Homo sapiens)
NPC1 structure in GDN micelles at pH 8.0
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6w5s
Q-score: 0.541
Qian H, Wu X, Du X, Yao X, Zhao X, Lee J, Yang H, Yan N
Cell (2020) 182 pp. 98-111.e18 [ DOI: doi:10.1016/j.cell.2020.05.020 Pubmed: 32544384 ]
- Cholesterol (386 Da, Ligand)
- Npc intracellular cholesterol transporter 1 (146 kDa, Protein from Homo sapiens)
- Npc1 at ph 8.0 (150 kDa, Complex from Homo sapiens)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
NPC1 structure in GDN micelles at pH 5.5, conformation a
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6w5t
Q-score: 0.421
Qian H, Wu X, Du X, Yao X, Zhao X, Lee J, Yang H, Yan N
Cell (2020) 182 pp. 98-111.e18 [ DOI: doi:10.1016/j.cell.2020.05.020 Pubmed: 32544384 ]
- Cholesterol (386 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Npc1 in gdn micelles at ph 5.5 (150 kDa, Complex from Homo sapiens)
- Npc intracellular cholesterol transporter 1 (146 kDa, Protein from Homo sapiens)
NPC1 structure in GDN micelles at pH 5.5, conformation b
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6w5u
Q-score: 0.405
Qian H, Wu X, Du X, Yao X, Zhao X, Lee J, Yang H, Yan N
Cell (2020) 182 pp. 98-111.e18 [ DOI: doi:10.1016/j.cell.2020.05.020 Pubmed: 32544384 ]
- Npc1 in gdn micelles at ph 5.5 (150 kDa, Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Npc intracellular cholesterol transporter 1 (146 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
NPC1-NPC2 complex structure at pH 5.5
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6w5v
Q-score: 0.358
Qian H, Wu X, Du X, Yao X, Zhao X, Lee J, Yang H, Yan N
Cell (2020) 182 pp. 98-111.e18 [ DOI: doi:10.1016/j.cell.2020.05.020 Pubmed: 32544384 ]
- Npc intracellular cholesterol transporter 2 (17 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Npc1-npc2 complex at ph 5.5 (170 kDa, Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Npc intracellular cholesterol transporter 1 (146 kDa, Protein from Homo sapiens)
Structure of RyR2 (Ca2+ alone dataset)
Resolution: 6.1 Å
EM Method: Single-particle
Fitted PDBs: 6jg3
Q-score: 0.184
Chi X, Gong D, Ren K, Zhou G, Huang G, Lei J, Zhou Q, Yan N
PNAS (2019) 116 pp. 25575-25582 [ Pubmed: 31792195 DOI: doi:10.1073/pnas.1914451116 ]
- Homotetramer (Complex from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Ryanodine receptor 2 (564 kDa, Protein from Sus scrofa)
Structure of RyR2 (F/P/Ca2+ dataset)
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 6jgz
Q-score: 0.249
Chi X, Gong D, Ren K, Zhou G, Huang G, Lei J, Zhou Q, Yan N
PNAS (2019) 116 pp. 25575-25582 [ Pubmed: 31792195 DOI: doi:10.1073/pnas.1914451116 ]
- Peptidyl-prolyl cis-trans isomerase fkbp1b (11 kDa, Protein from Homo sapiens)
- Ryr2 (564 kDa, Protein from Sus scrofa)
- Ryr2 in complex with fkbp12.6 (Complex from Sus scrofa)
- Zinc ion (65 Da, Ligand)