Cryo-EM structure of tetrameric Ptch1 in complex with ShhNp (form I)
Resolution: 6.8 Å
EM Method: Single-particle
Fitted PDBs: 6n7g
Q-score: 0.132
Qian H, Cao P, Hu M, Gao S, Yan N, Gong X
Nat Commun (2019) 10 pp. 2320-2320 [ DOI: doi:10.1038/s41467-019-10234-9 Pubmed: 31127104 ]
- Zinc ion (65 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Patch1 (Cellular component from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Protein patched homolog 1 (150 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Sonic hedgehog protein (19 kDa, Protein from Homo sapiens)
Cryo-EM structure of the 2:1 hPtch1-Shhp complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6n7h
Q-score: 0.408
Qian H, Cao P, Hu M, Gao S, Yan N, Gong X
Nat Commun (2019) 10 pp. 2320-2320 [ DOI: doi:10.1038/s41467-019-10234-9 Pubmed: 31127104 ]
- Zinc ion (65 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Patch1 (Cellular component from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Protein patched homolog 1 (150 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Sonic hedgehog protein (19 kDa, Protein from Homo sapiens)
Cryo-EM structure of tetrameric Ptch1 in complex with ShhNp (form II)
Resolution: 6.5 Å
EM Method: Single-particle
Fitted PDBs: 6n7k
Q-score: 0.132
Qian H, Cao P, Hu M, Gao S, Yan N, Gong X
Nat Commun (2019) 10 pp. 2320-2320 [ DOI: doi:10.1038/s41467-019-10234-9 Pubmed: 31127104 ]
- Zinc ion (65 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Patch1 (Cellular component from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Protein patched homolog 1 (150 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Sonic hedgehog protein (19 kDa, Protein from Homo sapiens)
Cryo-EM structure of human Ptch1
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6dmb
Q-score: 0.408
Gong X, Qian H, Cao P, Zhao X, Zhou Q, Lei J, Yan N
Science (2018) 361 [ Pubmed: 29954986 DOI: doi:10.1126/science.aas8935 ]
- Cholesterol hemisuccinate (486 Da, Ligand)
- Protein patched homolog 1 (150 kDa, Protein from Homo sapiens)
- Patch1 (Cellular component from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Cryo-EM structure of human Ptch1 with three mutations L282Q/T500F/P504L
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6dmo
Q-score: 0.279
Gong X, Qian H, Cao P, Zhao X, Zhou Q, Lei J, Yan N
Science (2018) 361 [ Pubmed: 29954986 DOI: doi:10.1126/science.aas8935 ]
- Patch1 with l282q/t500f/p504l mutations (Cellular component from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Protein patched homolog 1 (150 kDa, Protein from Homo sapiens)
Cryo-EM structure of human Ptch1 and ShhN complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6dmy
Q-score: 0.388
Gong X, Qian H, Cao P, Zhao X, Zhou Q, Lei J, Yan N
Science (2018) 361 [ Pubmed: 29954986 DOI: doi:10.1126/science.aas8935 ]
- Serine (105 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Patch1 (Cellular component from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Sonic hedgehog protein (19 kDa, Protein from Homo sapiens)
- Protein patched homolog 1 (150 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
cryo-EM map of the full-length human NPC1 in complex with the cleaved glycoprotein of Ebola virus
Resolution: 6.56 Å
EM Method: Single-particle
Fitted PDBs: 5jnx
Q-score: 0.14
Gong X, Qian H, Zhou X, Wu J, Wan T, Cao P, Huang W, Zhao X, Wang X, Wang P, Shi Y, Gao GF, Zhou Q, Yan N
Cell (2016) 165 pp. 1467-1478 [ DOI: doi:10.1016/j.cell.2016.05.022 Pubmed: 27238017 ]
- Npc1 in complex with ebov-gpcl trimer (300 kDa, Complex from Homo sapiens)
- Npc1 in complex with ebov-gpcl trimer (Protein from Homo sapiens)