CryoEM structure of LARGE1 bound to UDP
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 9e1t
Q-score: 0.472
Joesph S, Spellmon N, Schnicker NJ, Xu Z, Yang T, Hopkins J, Chakravarthy S, Davulcu O, Anderson ME, Venzke D, Campbell KP
To Be Published
- Xylosyl- and glucuronyltransferase large1 (89 kDa, Protein from Homo sapiens)
- Large1 (Complex from Homo sapiens)
- Uridine-5'-diphosphate (404 Da, Ligand)
- Manganese (ii) ion (54 Da, Ligand)
Resolution: 2.41 Å
EM Method: Single-particle
Fitted PDBs: 9ctq
Q-score: 0.659
Wang J, Owji AP, Kittredge A, Clark Z, Zhang Y, Yang T
Nat Commun (2024) 15 pp. 8051-8051 [ Pubmed: 39277606 DOI: doi:10.1038/s41467-024-52039-5 ]
- Calcium ion (40 Da, Ligand)
- Bestrophin-1 (67 kDa, Protein from Homo sapiens)
- Gamma-amino-butanoic acid (103 Da, Ligand)
- Gaba-bound homopentameric complex of best1 anion channel in fully open fffff state (337 kDa, Complex from Homo sapiens)
Best1 + GABA intermediate state 1
Resolution: 2.42 Å
EM Method: Single-particle
Fitted PDBs: 9ctr
Q-score: 0.651
Wang J, Owji AP, Kittredge A, Clark Z, Zhang Y, Yang T
Nat Commun (2024) 15 pp. 8051-8051 [ Pubmed: 39277606 DOI: doi:10.1038/s41467-024-52039-5 ]
- Calcium ion (40 Da, Ligand)
- Gaba-bound homopentameric complex of best1 anion channel in intermediate state 1 icccc (337 kDa, Complex from Homo sapiens)
- Gamma-amino-butanoic acid (103 Da, Ligand)
- Bestrophin-1 (67 kDa, Protein from Homo sapiens)
Best1 + GABA intermediate state 2
Resolution: 2.45 Å
EM Method: Single-particle
Fitted PDBs: 9cts
Q-score: 0.64
Wang J, Owji AP, Kittredge A, Clark Z, Zhang Y, Yang T
Nat Commun (2024) 15 pp. 8051-8051 [ Pubmed: 39277606 DOI: doi:10.1038/s41467-024-52039-5 ]
- Gaba-bound homopentameric complex of best1 anion channel in intermediate state 2 icicc (337 kDa, Complex from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Bestrophin-1 (67 kDa, Protein from Homo sapiens)
- Gamma-amino-butanoic acid (103 Da, Ligand)
cryoEM structure of bovine bestrophin-2 and glutamine synthetase complex
Resolution: 2.0 Å
EM Method: Single-particle
Fitted PDBs: 8ecy
Q-score: 0.7
Owji AP, Yu K, Kittredge A, Wang J, Zhang Y, Yang T
Nature (2022) 611 pp. 180-187 [ DOI: doi:10.1038/s41586-022-05373-x Pubmed: 36289327 ]
- Water (18 Da, Ligand)
- Manganese (ii) ion (54 Da, Ligand)
- Best2-gs (Complex from Homo sapiens)
- Bestrophin (47 kDa, Protein from Bos taurus)
- Glutamine synthetase (42 kDa, Protein from Bos taurus)
- Chloride ion (35 Da, Ligand)
- Calcium ion (40 Da, Ligand)
hBest2 1uM Ca2+ (Ca2+-bound) closed state
Resolution: 1.78 Å
EM Method: Single-particle
Fitted PDBs: 8d1e
Q-score: 0.789
Owji AP, Wang J, Kittredge A, Clark Z, Zhang Y, Hendrickson WA, Yang T
Nat Commun (2022) 13 pp. 3836-3836 [ Pubmed: 35789156 DOI: doi:10.1038/s41467-022-31437-7 ]
- Water (18 Da, Ligand)
- Hbest2 1um ca2+ (ca2+-bound) closed state (234 kDa, Complex from Homo sapiens)
- Chloride ion (35 Da, Ligand)
- 2-[2-[(1~{s},2~{s},4~{s},5'~{r},6~{r},7~{s},8~{r},9~{s},12~{s},13~{r},16~{s})-5',7,9,13-tetramethylspiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icos-18-ene-6,2'-oxane]-16-yl]oxyethyl]propane-1,3-diol (516 Da, Ligand)
- 1,2-dimyristoyl-rac-glycero-3-phosphocholine (677 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Bestrophin-2 (46 kDa, Protein from Homo sapiens)
hBest2 5mM Ca2+ (Ca2+-bound) closed state
Resolution: 1.82 Å
EM Method: Single-particle
Fitted PDBs: 8d1f
Q-score: 0.787
Owji AP, Wang J, Kittredge A, Clark Z, Zhang Y, Hendrickson WA, Yang T
Nat Commun (2022) 13 pp. 3836-3836 [ Pubmed: 35789156 DOI: doi:10.1038/s41467-022-31437-7 ]
- Water (18 Da, Ligand)
- Chloride ion (35 Da, Ligand)
- 2-[2-[(1~{s},2~{s},4~{s},5'~{r},6~{r},7~{s},8~{r},9~{s},12~{s},13~{r},16~{s})-5',7,9,13-tetramethylspiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icos-18-ene-6,2'-oxane]-16-yl]oxyethyl]propane-1,3-diol (516 Da, Ligand)
- 1,2-dimyristoyl-rac-glycero-3-phosphocholine (677 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Hbest2 5mm ca2+ (ca2+-bound) closed state (234 kDa, Complex from Homo sapiens)
- Bestrophin-2 (46 kDa, Protein from Homo sapiens)
Resolution: 2.07 Å
EM Method: Single-particle
Fitted PDBs: 8d1g
Q-score: 0.703
Owji AP, Wang J, Kittredge A, Clark Z, Zhang Y, Hendrickson WA, Yang T
Nat Commun (2022) 13 pp. 3836-3836 [ Pubmed: 35789156 DOI: doi:10.1038/s41467-022-31437-7 ]
- Water (18 Da, Ligand)
- Chloride ion (35 Da, Ligand)
- 2-[2-[(1~{s},2~{s},4~{s},5'~{r},6~{r},7~{s},8~{r},9~{s},12~{s},13~{r},16~{s})-5',7,9,13-tetramethylspiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icos-18-ene-6,2'-oxane]-16-yl]oxyethyl]propane-1,3-diol (516 Da, Ligand)
- 1,2-dimyristoyl-rac-glycero-3-phosphocholine (677 Da, Ligand)
- Hbest2 ca2+-bound open state (234 kDa, Complex from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- Bestrophin-2 (46 kDa, Protein from Homo sapiens)
hBest2 Ca2+-unbound closed state
Resolution: 1.94 Å
EM Method: Single-particle
Fitted PDBs: 8d1h
Q-score: 0.78
Owji AP, Wang J, Kittredge A, Clark Z, Zhang Y, Hendrickson WA, Yang T
Nat Commun (2022) 13 pp. 3836-3836 [ Pubmed: 35789156 DOI: doi:10.1038/s41467-022-31437-7 ]
- Water (18 Da, Ligand)
- Chloride ion (35 Da, Ligand)
- 2-[2-[(1~{s},2~{s},4~{s},5'~{r},6~{r},7~{s},8~{r},9~{s},12~{s},13~{r},16~{s})-5',7,9,13-tetramethylspiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icos-18-ene-6,2'-oxane]-16-yl]oxyethyl]propane-1,3-diol (516 Da, Ligand)
- 1,2-dimyristoyl-rac-glycero-3-phosphocholine (677 Da, Ligand)
- Hbest2 ca2+-unbound closed state (234 kDa, Complex from Homo sapiens)
- Bestrophin-2 (46 kDa, Protein from Homo sapiens)
hBest1 1uM Ca2+ (Ca2+-bound) closed state
Resolution: 1.82 Å
EM Method: Single-particle
Fitted PDBs: 8d1i
Q-score: 0.786
Owji AP, Wang J, Kittredge A, Clark Z, Zhang Y, Hendrickson WA, Yang T
Nat Commun (2022) 13 pp. 3836-3836 [ Pubmed: 35789156 DOI: doi:10.1038/s41467-022-31437-7 ]
- Water (18 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Bestrophin-1 (67 kDa, Protein from Homo sapiens)
- 1,2-dimyristoyl-rac-glycero-3-phosphocholine (677 Da, Ligand)
- Hbest1 1um ca2+ (ca2+-bound) closed state (338 kDa, Complex from Homo sapiens)