Structure of the native microtubule lattice nucleated from the yeast spindle pole body
Resolution: 6.6 Å
EM Method: Subtomogram averaging
Fitted PDBs: 8qv0
Q-score: 0.123
Dendooven T, Yatskevich S, Burt A, Chen ZA, Bellini D, Rappsilber J, Kilmartin JV, Barford D
Nat Struct Mol Biol (2024) 31 pp. 1134-1144 [ Pubmed: 38609662 DOI: doi:10.1038/s41594-024-01281-y ]
Resolution: 9.2 Å
EM Method: Subtomogram averaging
Fitted PDBs: 8qv2
Q-score: 0.112
Dendooven T, Yatskevich S, Burt A, Chen ZA, Bellini D, Rappsilber J, Kilmartin JV, Barford D
Nat Struct Mol Biol (2024) 31 pp. 1134-1144 [ Pubmed: 38609662 DOI: doi:10.1038/s41594-024-01281-y ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Water (18 Da, Ligand)
- Tubulin alpha-1 chain (49 kDa, Protein from Saccharomyces cerevisiae)
- Spindle pole body component (98 kDa, Protein from Saccharomyces cerevisiae)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Spindle pole body component 110 (111 kDa, Protein from Saccharomyces cerevisiae)
- Tubulin gamma chain (52 kDa, Protein from Saccharomyces cerevisiae)
- Unkown protein (5 kDa, Protein from Saccharomyces cerevisiae)
- Spindle pole body component (96 kDa, Protein from Saccharomyces cerevisiae)
- Y-tubulin ring complex capping the microtubule minus end (Cellular component from Saccharomyces cerevisiae)
- Tubulin beta chain (50 kDa, Protein from Saccharomyces cerevisiae)
Resolution: 8.2 Å
EM Method: Subtomogram averaging
Fitted PDBs: 8qv3
Q-score: 0.144
Dendooven T, Yatskevich S, Burt A, Chen ZA, Bellini D, Rappsilber J, Kilmartin JV, Barford D
Nat Struct Mol Biol (2024) 31 pp. 1134-1144 [ Pubmed: 38609662 DOI: doi:10.1038/s41594-024-01281-y ]
- Water (18 Da, Ligand)
- Y-tubulin small complex bound to two alpha/beta tubulin dimers (Cellular component from Saccharomyces cerevisiae)
- Unknown protein (5 kDa, Protein from Saccharomyces cerevisiae)
- Tubulin alpha-1 chain (49 kDa, Protein from Saccharomyces cerevisiae)
- Spindle pole body component (98 kDa, Protein from Saccharomyces cerevisiae)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Spindle pole body component 110 (111 kDa, Protein from Saccharomyces cerevisiae)
- Tubulin gamma chain (52 kDa, Protein from Saccharomyces cerevisiae)
- Unknown protein (5 kDa, Protein from Saccharomyces cerevisiae)
- Spindle pole body component (96 kDa, Protein from Saccharomyces cerevisiae)
- Tubulin beta chain (50 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of S.cerevisiae cohesin-Scc2-DNA complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6zz6
Q-score: 0.439
Collier JE, Lee BG, Roig MB, Yatskevich S, Petela NJ, Metson J, Voulgaris M, Gonzalez Llamazares A, Lowe J, Nasmyth KA
eLife (2020) 9 [ Pubmed: 32930661 DOI: doi:10.7554/eLife.59560 ]
- Structural maintenance of chromosomes protein 1,structural maintenance of chromosomes protein 1,structural maintenance of chromosomes protein 1 (40 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (34-mer) (10 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Complex of cohesin and scc2 (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Double strand dna (Complex from synthetic construct)
- Sister chromatid cohesion protein 2 (171 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (34-mer) (10 kDa, DNA from synthetic construct)
- Structural maintenance of chromosomes protein 3,structural maintenance of chromosomes protein 3,structural maintenance of chromosomes protein 3 (48 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Sister chromatid cohesion protein 1,sister chromatid cohesion protein 1,sister chromatid cohesion protein 1 (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Complex of cohesin, scc2, atp and dna (485 kDa, Complex)