Structure of the human outer kinetochore KMN network complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8ppr
Q-score: 0.341
Yatskevich S, Yang J, Bellini D, Zhang Z, Barford D
Nat Struct Mol Biol (2024) 31 pp. 874-883 [ Pubmed: 38459127 DOI: doi:10.1038/s41594-024-01249-y ]
- Kinetochore scaffold 1 (265 kDa, Protein from Homo sapiens)
- Outer kinetochore kmn network junction complex (Complex from Homo sapiens)
- Kinetochore-associated protein dsn1 homolog (40 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Zw10 interactor (31 kDa, Protein from Homo sapiens)
- Kinetochore protein spc24 (22 kDa, Protein from Homo sapiens)
- Protein mis12 homolog (24 kDa, Protein from Homo sapiens)
- Polyamine-modulated factor 1 (23 kDa, Protein from Homo sapiens)
- Kinetochore-associated protein nsl1 homolog (32 kDa, Protein from Homo sapiens)
- Kinetochore protein spc25 (26 kDa, Protein from Homo sapiens)
Cryo-EM structure of CBF1-CCAN bound topologically to centromeric DNA
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8ovw
Q-score: 0.386
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ Pubmed: 37506202 DOI: doi:10.1126/sciadv.adg7480 ]
- Inner kinetochore subunit nkp2 (17 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit iml3 (28 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit okp1 (47 kDa, Protein from Saccharomyces cerevisiae)
- A complex of cbf1-ccan bound to centromeric c0n3 dna (Complex from Saccharomyces cerevisiae)
- Inner kinetochore subunit wip1 (10 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ame1 (37 kDa, Protein from Saccharomyces cerevisiae)
- C0n3 dna (47 kDa, DNA from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf19 (42 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm21 (43 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Centromere-binding protein 1 (39 kDa, Protein from Saccharomyces cerevisiae)
- C0n3 dna (47 kDa, DNA from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm22 (27 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf3 (84 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit chl4 (52 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit cnn1 (41 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm16 (21 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of yeast CENP-OPQU+ bound to the CENP-A N-terminus
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8ovx
Q-score: 0.283
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ Pubmed: 37506202 DOI: doi:10.1126/sciadv.adg7480 ]
- Inner kinetochore subunit nkp2 (17 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit okp1 (47 kDa, Protein from Saccharomyces cerevisiae)
- A complex of cbf1-ccan bound to centromeric c0n3 dna (Complex from Saccharomyces cerevisiae)
- Inner kinetochore subunit ame1 (37 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf19 (42 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm21 (43 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp1 (27 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of CBF1-CCAN bound topologically to a centromeric CENP-A nucleosome
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8ow0
Q-score: 0.2
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ Pubmed: 37506202 DOI: doi:10.1126/sciadv.adg7480 ]
- Inner kinetochore subunit nkp2 (17 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp1 (27 kDa, Protein from Saccharomyces cerevisiae)
- C0n3 dna (47 kDa, DNA from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf3 (84 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit chl4 (52 kDa, Protein from Saccharomyces cerevisiae)
- Histone h4 (11 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit okp1 (47 kDa, Protein from Saccharomyces cerevisiae)
- Histone h2b.1 (14 kDa, Protein from Saccharomyces cerevisiae)
- A complex of cbf1-ccan bound to centromeric c0n3 dna (Complex from Saccharomyces cerevisiae)
- Inner kinetochore subunit wip1 (10 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit cnn1 (41 kDa, Protein from Saccharomyces cerevisiae)
- Histone h3-like centromeric protein cse4 (26 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit iml3 (28 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm21 (43 kDa, Protein from Saccharomyces cerevisiae)
- Centromere-binding protein 1 (39 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm22 (27 kDa, Protein from Saccharomyces cerevisiae)
- Histone h2a.1 (14 kDa, Protein from Saccharomyces cerevisiae)
- C0n3 dna (47 kDa, DNA from Saccharomyces cerevisiae)
- Inner kinetochore subunit ame1 (37 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf19 (42 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm16 (21 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of the yeast Inner kinetochore bound to a CENP-A nucleosome.
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8ow1
Q-score: 0.165
Dendooven T, Zhang Z, Yang J, McLaughlin SH, Schwab J, Scheres SHW, Yatskevich S, Barford D
Sci Adv (2023) 9 pp. eadg7480-eadg7480 [ Pubmed: 37506202 DOI: doi:10.1126/sciadv.adg7480 ]
- Suppressor of kinetochore protein 1 (22 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp2 (17 kDa, Protein from Saccharomyces cerevisiae)
- Centromere dna-binding protein complex cbf3 subunit b (71 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit nkp1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf3 (84 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit chl4 (52 kDa, Protein from Saccharomyces cerevisiae)
- Histone h4 (11 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit okp1 (47 kDa, Protein from Saccharomyces cerevisiae)
- Histone h2b.1 (14 kDa, Protein from Saccharomyces cerevisiae)
- A complex of cbf1-ccan bound to centromeric c0n3 dna (Complex from Saccharomyces cerevisiae)
- Centromere dna-binding protein complex cbf3 subunit c (56 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit wip1 (10 kDa, Protein from Saccharomyces cerevisiae)
- C0n3 (47 kDa, DNA from Saccharomyces cerevisiae)
- Inner kinetochore subunit cnn1 (41 kDa, Protein from Saccharomyces cerevisiae)
- Histone h3-like centromeric protein cse4 (26 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit iml3 (28 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm21 (43 kDa, Protein from Saccharomyces cerevisiae)
- C0n3 (47 kDa, DNA from Saccharomyces cerevisiae)
- Centromere-binding protein 1 (39 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm22 (27 kDa, Protein from Saccharomyces cerevisiae)
- Histone h2a.1 (14 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ame1 (37 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit ctf19 (42 kDa, Protein from Saccharomyces cerevisiae)
- Inner kinetochore subunit mcm16 (21 kDa, Protein from Saccharomyces cerevisiae)
Structure of the human CCAN deltaCT complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7pkn
Q-score: 0.319
Yatskevich S, Muir KW, Bellini D, Zhang Z, Yang J, Tischer T, Predin M, Dendooven T, McLaughlin SH, Barford D
Science (2022) 376 pp. 844-852 [ DOI: doi:10.1126/science.abn3810 Pubmed: 35420891 ]
- Centromere protein h (28 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Centromere protein n (39 kDa, Protein from Homo sapiens)
- Centromere protein m (19 kDa, Protein from Homo sapiens)
- Human inner kinetochore ccan (Complex from Homo sapiens)
- Centromere protein k (31 kDa, Protein from Homo sapiens)
- Centromere protein o (33 kDa, Protein from Homo sapiens)
- Centromere protein i (86 kDa, Protein from Homo sapiens)
- Centromere protein p (33 kDa, Protein from Homo sapiens)
- Centromere protein q (30 kDa, Protein from Homo sapiens)
- Centromere protein u (47 kDa, Protein from Homo sapiens)
- Centromere protein r (20 kDa, Protein from Homo sapiens)
- Centromere protein l (39 kDa, Protein from Homo sapiens)
SUMOylated apoAPC/C with repositioned APC2 WHB domain
Resolution: 3.78 Å
EM Method: Single-particle
Fitted PDBs: 6tnt
Q-score: 0.39
Yatskevich S, Kroonen JS, Alfieri C, Tischer T, Howes AC, Clijsters L, Yang J, Zhang Z, Yan K, Vertegaal ACO, Barford D
Cell Rep (2021) 34 pp. 108929-108929 [ Pubmed: 33789095 DOI: doi:10.1016/j.celrep.2021.108929 ]
- Anaphase-promoting complex subunit 5 (85 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 11 (9 kDa, Protein from Homo sapiens)
- Unidentified peptide (Complex from unidentified)
- Anaphase-promoting complex subunit 15 (14 kDa, Protein from Homo sapiens)
- Body 2 with repositioned apc2 whb domain after multi body refinement (Complex from Homo sapiens)
- Anaphase-promoting complex subunit cdc26 (9 kDa, Protein from Homo sapiens)
- Sumoylated apoapc/c with repositioned apc2 whb domain (Complex)
- Anaphase-promoting complex subunit 4 (92 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 1 (216 kDa, Protein from Homo sapiens)
- Unidentified peptide (1 kDa, Protein from unidentified)
- Zinc ion (65 Da, Ligand)
- Anaphase-promoting complex subunit 13 (8 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 7 (66 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 2 (93 kDa, Protein from Homo sapiens)
- Cell division cycle protein 27 homolog (91 kDa, Protein from Homo sapiens)
- Cell division cycle protein 16 homolog (71 kDa, Protein from Homo sapiens)
- Cell division cycle protein 23 homolog (68 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 10 (21 kDa, Protein from Homo sapiens)
- Anaphase-promoting complex subunit 16 (11 kDa, Protein from Homo sapiens)
Cryo-EM structure of S.cerevisiae cohesin-Scc2-DNA complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6zz6
Q-score: 0.439
Collier JE, Lee BG, Roig MB, Yatskevich S, Petela NJ, Metson J, Voulgaris M, Gonzalez Llamazares A, Lowe J, Nasmyth KA
eLife (2020) 9 [ Pubmed: 32930661 DOI: doi:10.7554/eLife.59560 ]
- Structural maintenance of chromosomes protein 1,structural maintenance of chromosomes protein 1,structural maintenance of chromosomes protein 1 (40 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (34-mer) (10 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Complex of cohesin and scc2 (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Double strand dna (Complex from synthetic construct)
- Sister chromatid cohesion protein 2 (171 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (34-mer) (10 kDa, DNA from synthetic construct)
- Structural maintenance of chromosomes protein 3,structural maintenance of chromosomes protein 3,structural maintenance of chromosomes protein 3 (48 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Sister chromatid cohesion protein 1,sister chromatid cohesion protein 1,sister chromatid cohesion protein 1 (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Complex of cohesin, scc2, atp and dna (485 kDa, Complex)