DNA helicase RECQL5 in complex with homo Di-Gluebody G5-006
Resolution: 3.79 Å
EM Method: Single-particle
Fitted PDBs: 8rl5
Q-score: 0.217
Yi G, Mamalis D, Ye M, Carrique L, Fairhead M, Li H, Duerr K, Zhang P, Sauer DB, von Delft F, Davis BG, Gilbert RJC
To Be Published
- Zinc ion (65 Da, Ligand)
- Recql5 in complex with g5-006 homo di-gluebody (126 kDa, Complex)
- Gluebody g5-006 (13 kDa, Protein from Lama glama)
- Atp-dependent dna helicase q5 (Complex from Homo sapiens)
- Atp-dependent dna helicase q5 (49 kDa, Protein from Homo sapiens)
- Di-gluebody g5-006 (Complex from Lama glama)
DNA helicase RECQL5 in complex with homo Di-Gluebody G5-006 - RECQL5 local refinement
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 8rl6
Q-score: 0.22
Yi G, Mamalis D, Ye M, Carrique L, Fairhead M, Li H, Duerr K, Zhang P, Sauer DB, von Delft F, Davis BG, Gilbert RJC
To Be Published
- Local refinement of the recql5 part of recql5 homodimer assembled by g5-006 homo di-gluebody (126 kDa, Complex from Homo sapiens)
- Atp-dependent dna helicase q5 (49 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
SPNS2:sfGFP hetero dimer assembled by Di-Gluebody - SPNS2 local refinement
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 8rld
Q-score: 0.417
Yi G, Mamalis D, Ye M, Carrique L, Fairhead M, Li H, Duerr K, Zhang P, Sauer DB, von Delft F, Davis BG, Gilbert RJC
To Be Published
SPNS2:sfGFP hetero dimer assembled by Di-Gluebody - sfGFP local refinement
Resolution: 3.75 Å
EM Method: Single-particle
Fitted PDBs: 8rle
Q-score: 0.275
Yi G, Mamalis D, Ye M, Carrique L, Fairhead M, Li H, Duerr K, Zhang P, Sauer DB, von Delft F, Davis BG, Gilbert RJC
To Be Published
- Local refinement of gfp part of the spns2:sfgfp heterodimer assembled by di-gluebody (112 kDa, Complex)
- Green fluorescent protein (Complex from Aequorea victoria)
- Di-gluebody (Complex from Lama glama)
- Green fluorescent protein (26 kDa, Protein from Aequorea victoria)
- Gluebody gbc4 (12 kDa, Protein from Lama glama)
- Gluebody gbenhancer (12 kDa, Protein from Lama glama)
Resolution: 3.76 Å
EM Method: Single-particle
Fitted PDBs: 8opp
Q-score: 0.245
Yi G, Ye M, Carrique L, El-Sagheer A, Brown T, Norbury CJ, Zhang P, Gilbert RJC
Nat Struct Mol Biol (2024) 31 pp. 1426-1438 [ DOI: doi:10.1038/s41594-024-01357-9 Pubmed: 39054354 ]
- [[(2~{r},3~{s},4~{r},5~{r})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-sulfanyl-phosphoryl] phosphono hydrogen phosphate (500 Da, Ligand)
- Rna (25-mer) (8 kDa, RNA from Homo sapiens)
- Terminal uridylyltransferase 7 (Complex from Homo sapiens)
- Rna (25-mer) (Complex from Homo sapiens)
- Human terminal urildylyltransferase 7 (tut7/zcchc6) bound with pre-let7g mirna (Complex)
- Terminal uridylyltransferase 7 (171 kDa, Protein from Homo sapiens)
Human terminal uridylyltransferase 7 (TUT7/ZCCHC6) bound with pre-let7g miRNA and Lin28A - complex 1
Resolution: 3.82 Å
EM Method: Single-particle
Fitted PDBs: 8ops
Q-score: 0.138
Yi G, Ye M, Carrique L, El-Sagheer A, Brown T, Norbury CJ, Zhang P, Gilbert RJC
Nat Struct Mol Biol (2024) 31 pp. 1426-1438 [ DOI: doi:10.1038/s41594-024-01357-9 Pubmed: 39054354 ]
- Human terminal uridylyltransferase 7 (tut7/zcchc6) bound with pre-let7g mirna and lin28a - complex 1 (Complex)
- Protein lin-28 homolog a (22 kDa, Protein from Homo sapiens)
- Rna (71-mer) let7g (22 kDa, RNA from Homo sapiens)
- Terminal uridylyltransferase 7 and protein lin-28 homolog a (Complex from Homo sapiens)
- Rna (71-mer) let7g (Complex from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Terminal uridylyltransferase 7 (171 kDa, Protein from Homo sapiens)
Human terminal uridylyltransferase 7 (TUT7/ZCCHC6) bound with pre-let7g miRNA and Lin28A - complex 2
Resolution: 3.65 Å
EM Method: Single-particle
Fitted PDBs: 8opt
Q-score: 0.26
Yi G, Ye M, Carrique L, El-Sagheer A, Brown T, Norbury CJ, Zhang P, Gilbert RJC
Nat Struct Mol Biol (2024) 31 pp. 1426-1438 [ DOI: doi:10.1038/s41594-024-01357-9 Pubmed: 39054354 ]
- Protein lin-28 homolog a (22 kDa, Protein from Homo sapiens)
- Terminal uridylyltransferase 7 and protein lin-28 homolog a (Complex from Homo sapiens)
- Rna (53-mer) (Complex from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Human terminal uridylyltransferase 7 (tut7/zcchc6) bound with pre-let7g mirna and lin28a - complex 2 (Complex)
- Rna (53-mer) (17 kDa, RNA from Homo sapiens)
- Terminal uridylyltransferase 7 (171 kDa, Protein from Homo sapiens)
MCM in complex with dsDNA in presence of ATP.
Resolution: 3.57 Å
EM Method: Single-particle
Fitted PDBs: 8x7u
Q-score: 0.347
Ma J, Yi G, Ye M, MacGregor-Chatwin C, Sheng Y, Lu Y, Li M, Li Q, Wang D, Gilbert RJC, Zhang P
Nat Commun (2024) 15 pp. 10304-10304 [ Pubmed: 39604363 DOI: doi:10.1038/s41467-024-53745-w ]
- Mcm homohexamer in complex with dsdna in presence of atp (Complex from Thermococcus kodakarensis)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Mini-chromosome maintenance complex 3 (76 kDa, Protein from Thermococcus kodakarensis)
MCM_ATP_dsDNA before local refinement
Ma J, Yi G, Ye M, MacGregor-Chatwin C, Sheng Y, Lu Y, Li M, Li Q, Wang D, Gilbert RJC, Zhang P
Nat Commun (2024) 15 pp. 10304-10304 [ Pubmed: 39604363 DOI: doi:10.1038/s41467-024-53745-w ]
local refinement of MCM_ATP_dsDNA
Ma J, Yi G, Ye M, MacGregor-Chatwin C, Sheng Y, Lu Y, Li M, Li Q, Wang D, Gilbert RJC, Zhang P
Nat Commun (2024) 15 pp. 10304-10304 [ Pubmed: 39604363 DOI: doi:10.1038/s41467-024-53745-w ]