Thermus thermophilus Rho-engaged RNAP elongation complex; EC part
Murayama Y, Ehara H, Aoki M, Goto M, Yokoyama T, Sekine SI
Sci Adv (2023) 9 pp. eade7093- [ Pubmed: 36753546 DOI: doi:10.1101/2022.08.29.505764 ]
Thermus thermophilus Rho-engaged RNAP elongation complex; Rho part
Murayama Y, Ehara H, Aoki M, Goto M, Yokoyama T, Sekine SI
Sci Adv (2023) 9 pp. eade7093- [ Pubmed: 36753546 DOI: doi:10.1101/2022.08.29.505764 ]
Thermus thermophilus RNA polymerase elongation complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8hsg
Q-score: 0.434
Murayama Y, Ehara H, Aoki M, Goto M, Yokoyama T, Sekine SI
Sci Adv (2023) 9 pp. eade7093-eade7093 [ Pubmed: 36753546 DOI: doi:10.1126/sciadv.ade7093 ]
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Thermus thermophilus HB8)
- Magnesium ion (24 Da, Ligand)
- Rna (5'-r(p*cp*cp*ap*cp*ap*up*cp*cp*ap*cp*cp*u)-3') (39 kDa, RNA from synthetic construct)
- Rna polymerase elongation complex (Complex from Thermus thermophilus HB8)
- Dna-directed rna polymerase subunit beta' (171 kDa, Protein from Thermus thermophilus HB8)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Thermus thermophilus HB8)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (125 kDa, Protein from Thermus thermophilus HB8)
- Dna (37-mer) (57 kDa, DNA from DNA molecule)
- Dna (27-mer) (54 kDa, DNA from synthetic construct)
Thermus thermophilus RNA polymerase coreenzyme
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8hsh
Q-score: 0.374
Murayama Y, Ehara H, Aoki M, Goto M, Yokoyama T, Sekine SI
Sci Adv (2023) 9 pp. eade7093-eade7093 [ Pubmed: 36753546 DOI: doi:10.1126/sciadv.ade7093 ]
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Thermus thermophilus HB8)
- Rna polymerase coreenzyme (Complex from Thermus thermophilus HB8)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (171 kDa, Protein from Thermus thermophilus HB8)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Thermus thermophilus HB8)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (125 kDa, Protein from Thermus thermophilus HB8)
Thermus thermophilus transcription termination factor Rho bound with ADP-BeF3
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8hsj
Q-score: 0.235
Murayama Y, Ehara H, Aoki M, Goto M, Yokoyama T, Sekine SI
Sci Adv (2023) 9 pp. eade7093-eade7093 [ Pubmed: 36753546 DOI: doi:10.1126/sciadv.ade7093 ]
- Beryllium trifluoride ion (66 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Transcription termination factor rho (47 kDa, Protein from Thermus thermophilus HB8)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Transcription termination factor rho (Complex from Thermus thermophilus HB8)
Thermus thermophilus RNA polymerase bound with an inverted Rho hexamer
Resolution: 5.8 Å
EM Method: Single-particle
Fitted PDBs: 8hsl
Q-score: 0.166
Murayama Y, Ehara H, Aoki M, Goto M, Yokoyama T, Sekine SI
Sci Adv (2023) 9 pp. eade7093-eade7093 [ Pubmed: 36753546 DOI: doi:10.1126/sciadv.ade7093 ]
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Thermus thermophilus HB8)
- Beryllium trifluoride ion (66 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Transcription termination factor rho (47 kDa, Protein from Thermus thermophilus HB8)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (171 kDa, Protein from Thermus thermophilus HB8)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (125 kDa, Protein from Thermus thermophilus HB8)
- Rna polymerase bound with an inverted rho (Complex from Thermus thermophilus HB8)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Thermus thermophilus HB8)
Thermus thermophilus Rho-engaged RNAP elongation complex (composite structure)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 8hsr
Q-score: 0.317
Murayama Y, Ehara H, Aoki M, Goto M, Yokoyama T, Sekine SI
Sci Adv (2023) 9 pp. eade7093-eade7093 [ Pubmed: 36753546 DOI: doi:10.1126/sciadv.ade7093 ]
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Thermus thermophilus HB8)
- Beryllium trifluoride ion (66 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Transcription termination factor rho (47 kDa, Protein from Thermus thermophilus HB8)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (171 kDa, Protein from Thermus thermophilus HB8)
- Rho-engaged rna polymerase elongation complex (Complex from Thermus thermophilus HB8)
- Dna (41-mer) (57 kDa, DNA from synthetic construct)
- Dna (31-mer) (54 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (125 kDa, Protein from Thermus thermophilus HB8)
- Rna (29-mer) (39 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Thermus thermophilus HB8)