Cryo-EM structure of S. cerevisiae PolE-core-DNA
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 9b8r
Q-score: 0.332
Yuan Z, Georgescu R, Yao NY, Yurieva O, O'Donnell ME, Li H
Science (2024) 385 pp. eadk5901-eadk5901 [ Pubmed: 39088616 DOI: doi:10.1126/science.adk5901 ]
- Pole-core-dna complex (Complex from Saccharomyces cerevisiae)
- Primer dna (3 kDa, DNA from DNA molecule)
- Dna polymerase epsilon catalytic subunit (133 kDa, Protein from Saccharomyces cerevisiae)
- Iron/sulfur cluster (351 Da, Ligand)
- Template dna (3 kDa, DNA from DNA molecule)
Cryo-EM structure of S. cerevisiae PolE-Ctf18-8-1-DNA
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8tw9
Q-score: 0.438
Yuan Z, Georgescu R, Yao NY, Yurieva O, O'Donnell ME, Li H
Science (2024) 385 pp. eadk5901-eadk5901 [ Pubmed: 39088616 DOI: doi:10.1126/science.adk5901 ]
- Dna polymerase epsilon catalytic subunit a (255 kDa, Protein from Saccharomyces cerevisiae)
- Chromosome transmission fidelity protein 18 (3 kDa, Protein from Saccharomyces cerevisiae)
- Iron/sulfur cluster (351 Da, Ligand)
- Primer dna (2 kDa, DNA from synthetic construct)
- Ctf18-rfc-pcna complex (Complex from Saccharomyces cerevisiae)
- Chromosome transmission fidelity protein 8 (15 kDa, Protein from Saccharomyces cerevisiae)
- Template dna (4 kDa, DNA from synthetic construct)
- Sister chromatid cohesion protein dcc1 (44 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of S. cerevisiae Ctf18-RFC-PCNA-PolE-DNA complex
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8twa
Q-score: 0.349
Yuan Z, Georgescu R, Yao NY, Yurieva O, O'Donnell ME, Li H
Science (2024) 385 pp. eadk5901-eadk5901 [ Pubmed: 39088616 DOI: doi:10.1126/science.adk5901 ]
- Dna polymerase epsilon catalytic subunit a (255 kDa, Protein from Saccharomyces cerevisiae)
- Chromosome transmission fidelity protein 18 (84 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 3 (36 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Chromosome transmission fidelity protein 18 (3 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Ctf18-rfc-pcna-pole-dna complex (Complex from Saccharomyces cerevisiae)
- Replication factor c subunit 4 (35 kDa, Protein from Saccharomyces cerevisiae)
- Iron/sulfur cluster (351 Da, Ligand)
- Proliferating cell nuclear antigen (28 kDa, Protein from Saccharomyces cerevisiae)
- Primer dna (2 kDa, DNA from synthetic construct)
- Replication factor c subunit 2 (38 kDa, Protein from Saccharomyces cerevisiae)
- Chromosome transmission fidelity protein 8 (15 kDa, Protein from Saccharomyces cerevisiae)
- Template dna (4 kDa, DNA from synthetic construct)
- Sister chromatid cohesion protein dcc1 (44 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of S. cerevisiae Ctf18-RFC-PCNA-DNA complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8twb
Q-score: 0.482
Yuan Z, Georgescu R, Yao NY, Yurieva O, O'Donnell ME, Li H
Science (2024) 385 pp. eadk5901-eadk5901 [ Pubmed: 39088616 DOI: doi:10.1126/science.adk5901 ]
- Chromosome transmission fidelity protein 18 (30 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Replication factor c subunit 3 (36 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Replication factor c subunit 4 (35 kDa, Protein from Saccharomyces cerevisiae)
- Template dna (5 kDa, DNA from synthetic construct)
- Primer dna (5 kDa, DNA from synthetic construct)
- Ctf18-rfc-pcna complex (Complex from Saccharomyces cerevisiae)
- Replication factor c subunit 2 (38 kDa, Protein from Saccharomyces cerevisiae)
- Proliferating cell nuclear antigen (28 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of S. cerevisiae DNA polymerase alpha-primase complex in the RNA synthesis state
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 8foh
Q-score: 0.358
Yuan Z, Georgescu R, Li H, O'Donnell ME
Nat Commun (2023) 14 pp. 3697-3697 [ DOI: doi:10.1038/s41467-023-39441-1 Pubmed: 37344454 ]
- Dna polymerase alpha/primase complex (Complex from Saccharomyces cerevisiae)
- Dna polymerase (167 kDa, Protein from Saccharomyces cerevisiae)
- Template dna (3 kDa, DNA from synthetic construct)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna primase large subunit (62 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase alpha subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
- Primer rna (2 kDa, RNA from synthetic construct)
- Dna primase (47 kDa, Protein from Saccharomyces cerevisiae)
Resolution: 4.8 Å
EM Method: Single-particle
Fitted PDBs: 8foj
Q-score: 0.336
Yuan Z, Georgescu R, Li H, O'Donnell ME
Nat Commun (2023) 14 pp. 3697-3697 [ DOI: doi:10.1038/s41467-023-39441-1 Pubmed: 37344454 ]
- Dna polymerase alpha/primase complex (Complex from Saccharomyces cerevisiae)
- Template dna (3 kDa, DNA from synthetic construct)
- Dna polymerase (167 kDa, Protein from Saccharomyces cerevisiae)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna primase large subunit (62 kDa, Protein from Saccharomyces cerevisiae)
- Primer rna (3 kDa, RNA from synthetic construct)
- Dna polymerase alpha subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
- Dna primase (47 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of S. cerevisiae DNA polymerase alpha-primase complex in the DNA elongation state
Resolution: 3.56 Å
EM Method: Single-particle
Fitted PDBs: 8fok
Q-score: 0.454
Yuan Z, Georgescu R, Li H, O'Donnell ME
Nat Commun (2023) 14 pp. 3697-3697 [ DOI: doi:10.1038/s41467-023-39441-1 Pubmed: 37344454 ]
- Dna polymerase alpha/primase complex (Complex from Saccharomyces cerevisiae)
- Rna-dna chimeric primer (4 kDa, RNA from synthetic construct)
- Dna polymerase (167 kDa, Protein from Saccharomyces cerevisiae)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna primase large subunit (62 kDa, Protein from Saccharomyces cerevisiae)
- Template dna (6 kDa, DNA from synthetic construct)
- Dna polymerase alpha subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
- Dna primase (47 kDa, Protein from Saccharomyces cerevisiae)
Structure of the S. cerevisiae replicative helicase CMG in complex with a forked DNA
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6u0m
Q-score: 0.219
Yuan Z, Georgescu R, Bai L, Zhang D, Li H, O'Donnell ME
Nat Commun (2020) 11 pp. 688- [ DOI: doi:10.1038/s41467-020-14577-6 ]
- Minichromosome maintenance protein 5 (74 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (74 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf2 (23 kDa, Protein from Saccharomyces cerevisiae)
- Dna (26-mer) (7 kDa, DNA from Saccharomyces cerevisiae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna (15-mer) (4 kDa, DNA from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf3 (21 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (82 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm4 (85 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (79 kDa, Protein from Saccharomyces cerevisiae)
- Cmg-forkdna (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (73 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein sld5 (33 kDa, Protein from Saccharomyces cerevisiae)
- Cell division control protein 45 (73 kDa, Protein from Saccharomyces cerevisiae)
Structure of Eukaryotic CMG Helicase at a Replication Fork and Implications
Resolution: 4.9 Å
EM Method: Single-particle
Fitted PDBs: 5u8t
Q-score: 0.239
Georgescu R, Yuan Z, Bai L, de Luna Almeida Santos R, Sun J, Zhang D, Yurieva O, Li H, O'Donnell ME
PNAS (2017) 114 pp. E697-E706 [ Pubmed: 28096349 DOI: doi:10.1073/pnas.1620500114 ]
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Cell division control protein 45 (74 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication complex gins protein psf2 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication complex gins protein sld5 (33 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Cmg-ssdna (750 kDa, Complex from Saccharomyces cerevisiae)
- Dna (5'-d(p*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*t)-3') (4 kDa, DNA from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf3 (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication complex gins protein psf1 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Structure of eukaryotic CMG helicase at a replication fork
Resolution: 6.1 Å
EM Method: Single-particle
Fitted PDBs: 5u8s
Q-score: 0.199
Georgescu R, Yuan Z, Bai L, de Luna Almeida Santos R, Sun J, Zhang D, Yurieva O, Li H, O'Donnell ME
PNAS (2017) 114 pp. E697-E706 [ Pubmed: 28096349 DOI: doi:10.1073/pnas.1620500114 ]
- Cmg-biotinylated fork dna-streptavidin (750 kDa, Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (26-mer) (7 kDa, DNA from synthetic construct)
- Cell division control protein 45 (74 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication complex gins protein psf1 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication complex gins protein psf3 (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication complex gins protein sld5 (33 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (5'-d(p*ap*tp*cp*gp*ap*tp*cp*gp*ap*tp*cp*gp*ap*t)-3') (4 kDa, DNA from synthetic construct)
- Dna replication complex gins protein psf2 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))