Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7try
Q-score: 0.338
Zhao LH, Lin J, Ji SY, Zhou XE, Mao C, Shen DD, He X, Xiao P, Sun J, Melcher K, Zhang Y, Yu X, Xu HE
Nat Commun (2022) 13 pp. 6670-6670 [ Pubmed: 36335102 DOI: doi:10.1038/s41467-022-33851-3 ]
- Corticotropin releasing factor receptor 2 (crf2r) bound to urocortin 1 (ucn1) and coupled with g11 proteins (Complex from Homo sapiens)
- G protein gamma subunit (7 kDa, Protein from Rattus norvegicus)
- Scfv16 (26 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein subunit alpha-11 (41 kDa, Protein from Homo sapiens)
- Corticotropin-releasing factor receptor 2 (63 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (43 kDa, Protein from Rattus norvegicus)
- Urocortin (4 kDa, Protein from Homo sapiens)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7ts0
Q-score: 0.494
Zhao LH, Lin J, Ji SY, Zhou XE, Mao C, Shen DD, He X, Xiao P, Sun J, Melcher K, Zhang Y, Yu X, Xu HE
Nat Commun (2022) 13 pp. 6670-6670 [ Pubmed: 36335102 DOI: doi:10.1038/s41467-022-33851-3 ]
- Dominant negative go alpha subunit (40 kDa, Protein from Homo sapiens)
- Corticotropin releasing factor receptor 2 (crf2r) bound to urocortin 1 (ucn1) and coupled with g11 proteins (Complex from Homo sapiens)
- Corticotropin-releasing factor receptor 2,corticotropin-releasing factor receptor 2,corticotropin-releasing factor receptor 2,corticotropin-releasing factor receptor 2,human corticotropin releasing factor receptor 2 (63 kDa, Protein from Homo sapiens)
- G protein gamma subunit (7 kDa, Protein from Rattus norvegicus)
- Scfv16 (26 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (43 kDa, Protein from Rattus norvegicus)
- Urocortin (4 kDa, Protein from Homo sapiens)
Cryo-EM structure of the human glucagon-like peptide-2 receptor-Gs protein complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7d68
Q-score: 0.524
Sun W, Chen LN, Zhou Q, Zhao LH, Yang D, Zhang H, Cong Z, Shen DD, Zhao F, Zhou F, Cai X, Chen Y, Zhou Y, Gadgaard S, van der Velden WJC, Zhao S, Jiang Y, Rosenkilde MM, Xu HE, Zhang Y, Wang MW
Cell Res (2020) 30 pp. 1098-1108 [ Pubmed: 33239759 DOI: doi:10.1038/s41422-020-00442-0 ]
- Guanine nucleotide-binding protein (Complex from Bos taurus)
- Water (18 Da, Ligand)
- Glucagon-like peptide 2 receptor (75 kDa, Protein from Homo sapiens)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Bos taurus)
- Pro-glucagon (3 kDa, Protein from Homo sapiens)
- Nanobody-35 (Complex from synthetic construct)
- Cryo-em structure of the human glucagon-like peptide-2 receptor-gs protein complex (Complex)
- Glucagon-like peptide 2 receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Bos taurus)
Cryo-EM structure of the human growth hormone-releasing hormone receptor-Gs protein complex
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7cz5
Q-score: 0.582
Zhou F, Zhang H, Cong Z, Zhao LH, Zhou Q, Mao C, Cheng X, Shen DD, Cai X, Ma C, Wang Y, Dai A, Zhou Y, Sun W, Zhao F, Zhao S, Jiang H, Jiang Y, Yang D, Eric Xu H, Zhang Y, Wang MW
Nat Commun (2020) 11 pp. 5205-5205 [ Pubmed: 33060564 DOI: doi:10.1038/s41467-020-18945-0 ]
- Somatoliberin (5 kDa, Protein from Homo sapiens)
- Hormone receptor, g(s) subunit alpha isoforms (Complex from Homo sapiens)
- G(s) subunit gamma-2 (Complex from Bos taurus)
- Hormone (Complex from Homo sapiens)
- Nanobody35 (Complex from synthetic construct)
- G(s) subunit beta-1 (Complex from Rattus norvegicus)
- Nanobody35 (13 kDa, Protein from synthetic construct)
- Palmitic acid (256 Da, Ligand)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Growth hormone-releasing hormone receptor,growth hormone-releasing hormone receptor (61 kDa, Protein from Homo sapiens)
- Growth hormone-releasing hormone receptor - gs protein complex (180 kDa, Complex)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (43 kDa, Protein from Rattus norvegicus)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6pb0
Q-score: 0.455
Ma S, Shen Q, Zhao LH, Mao C, Zhou XE, Shen DD, de Waal PW, Bi P, Li C, Jiang Y, Wang MW, Sexton PM, Wootten D, Melcher K, Zhang Y, Xu HE
Mol Cell (2020) 77 pp. 669- [ DOI: doi:10.1016/j.molcel.2020.01.013 Pubmed: 32004470 ]
- Nanobody 35 (15 kDa, Protein from synthetic construct)
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Corticotropin-releasing factor receptor 1 (43 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Urocortin (4 kDa, Protein from Homo sapiens)
- Urocortin1-bound crf1r in complex gs and nb35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 6pb1
Q-score: 0.47
Ma S, Shen Q, Zhao LH, Mao C, Zhou XE, Shen DD, de Waal PW, Bi P, Li C, Jiang Y, Wang MW, Sexton PM, Wootten D, Melcher K, Zhang Y, Xu HE
Mol Cell (2020) 77 pp. 669- [ DOI: doi:10.1016/j.molcel.2020.01.013 Pubmed: 32004470 ]
- Nanobody 35 (15 kDa, Protein from synthetic construct)
- Urocortin 1-bound corticotropin-releasing factor 2 receptor in complex with gs protein (Complex from Homo sapiens)
- Palmitic acid (256 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Urocortin (4 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short,guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (43 kDa, Protein from Homo sapiens)
- Corticotropin-releasing factor receptor 2 (45 kDa, Protein from Homo sapiens)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6nbf
Q-score: 0.444
Zhao LH, Ma S, Sutkeviciute I, Shen DD, Zhou XE, de Waal PW, Li CY, Kang Y, Clark LJ, Jean-Alphonse FG, White AD, Yang D, Dai A, Cai X, Chen J, Li C, Jiang Y, Watanabe T, Gardella TJ, Melcher K, Wang MW, Vilardaga JP, Xu HE, Zhang Y
Science (2019) 364 pp. 148-153 [ Pubmed: 30975883 DOI: doi:10.1126/science.aav7942 ]
- Parathyroid hormone/parathyroid hormone-related peptide receptor (54 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Palmitic acid (256 Da, Ligand)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Cryo-em structure of parathyroid hormone receptor type 1 in complex with a long-acting parathyroid hormone analog and g protein (Complex from Homo sapiens)
- Gs protein alpha subunit (43 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Long-acting parathyroid hormone analog (4 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6nbh
Q-score: 0.347
Zhao LH, Ma S, Sutkeviciute I, Shen DD, Zhou XE, de Waal PW, Li CY, Kang Y, Clark LJ, Jean-Alphonse FG, White AD, Yang D, Dai A, Cai X, Chen J, Li C, Jiang Y, Watanabe T, Gardella TJ, Melcher K, Wang MW, Vilardaga JP, Xu HE, Zhang Y
Science (2019) 364 pp. 148-153 [ Pubmed: 30975883 DOI: doi:10.1126/science.aav7942 ]
- Parathyroid hormone/parathyroid hormone-related peptide receptor (54 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Palmitic acid (256 Da, Ligand)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Cryo-em structure of parathyroid hormone receptor type 1 in complex with a long-acting parathyroid hormone analog and g protein (Complex from Homo sapiens)
- Gs protein alpha subunit (43 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Long-acting parathyroid hormone analog (4 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6nbi
Q-score: 0.285
Zhao LH, Ma S, Sutkeviciute I, Shen DD, Zhou XE, de Waal PW, Li CY, Kang Y, Clark LJ, Jean-Alphonse FG, White AD, Yang D, Dai A, Cai X, Chen J, Li C, Jiang Y, Watanabe T, Gardella TJ, Melcher K, Wang MW, Vilardaga JP, Xu HE, Zhang Y
Science (2019) 364 pp. 148-153 [ Pubmed: 30975883 DOI: doi:10.1126/science.aav7942 ]
- Parathyroid hormone/parathyroid hormone-related peptide receptor (54 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Nanobody-35 (13 kDa, Protein from synthetic construct)
- Cryo-em structure of parathyroid hormone receptor type 1 in complex with a long-acting parathyroid hormone analog and g protein (Complex from Homo sapiens)
- Gs protein alpha subunit (43 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Long-acting parathyroid hormone analog (4 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)