Cryo-EM structure of Cas8-HNH system at full R-loop state
Resolution: 2.51 Å
EM Method: Single-particle
Fitted PDBs: 8z0k
Q-score: 0.551
Li X, Liu Y, Han J, Zhang L, Liu Z, Wang L, Zhang S, Zhang Q, Fu P, Yin H, Zhu H, Zhang H
EMBO J (2024) 43 pp. 4656-4667 [ Pubmed: 39251884 DOI: doi:10.1038/s44318-024-00229-8 ]
- Hypothetical protein j6n51_11000 (28 kDa, Protein from Selenomonas sp.)
- Cryo-em structure of cas8-hnh system at full r-loop state (Complex from Selenomonas sp.)
- Rna (69-mer) (22 kDa, RNA from Selenomonas sp.)
- Type i-f crispr-associated endoribonuclease cas6/csy4 (20 kDa, Protein from Selenomonas sp.)
- Hnh endonuclease (39 kDa, Protein from Selenomonas sp.)
- Type i-f crispr-associated protein csy3 (37 kDa, Protein from Selenomonas sp.)
- Dna (5'-d(p*gp*tp*gp*cp*gp*gp*a)-3') (2 kDa, DNA from Selenomonas sp.)
- Dna (37-mer) (11 kDa, DNA from Selenomonas sp.)
Cryo-EM structure of Cas8-HNH system at partial R-loop state
Resolution: 2.57 Å
EM Method: Single-particle
Fitted PDBs: 8z0l
Q-score: 0.549
Li X, Liu Y, Han J, Zhang L, Liu Z, Wang L, Zhang S, Zhang Q, Fu P, Yin H, Zhu H, Zhang H
EMBO J (2024) 43 pp. 4656-4667 [ Pubmed: 39251884 DOI: doi:10.1038/s44318-024-00229-8 ]
- Hypothetical protein j6n51_11000 (28 kDa, Protein from Selenomonas sp.)
- Type i-f crispr-associated protein csy3 (37 kDa, Protein from Selenomonas sp.)
- Rna (69-mer) (22 kDa, RNA from Selenomonas sp.)
- Type i-f crispr-associated endoribonuclease cas6/csy4 (20 kDa, Protein from Selenomonas sp.)
- Cryo-em structure of cas8-hnh system at partial r-loop state (Complex from Selenomonas sp.)
- Hnh endonuclease (39 kDa, Protein from Selenomonas sp.)
- Dna (32-mer) (9 kDa, DNA from Selenomonas sp.)
- Dna (5'-d(p*gp*tp*gp*cp*gp*gp*a)-3') (2 kDa, DNA from Selenomonas sp.)
Cryo-EM structure of Cas8-HNH system at target free state
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8zdy
Q-score: 0.42
Li X, Liu Y, Han J, Zhang L, Liu Z, Wang L, Zhang S, Zhang Q, Fu P, Yin H, Zhu H, Zhang H
EMBO J (2024) 43 pp. 4656-4667 [ Pubmed: 39251884 DOI: doi:10.1038/s44318-024-00229-8 ]
- A protein (38 kDa, Protein from Selenomonas sp.)
- A protein (28 kDa, Protein from Selenomonas sp.)
- A protein (39 kDa, Protein from Selenomonas sp.)
- Rna (58-mer) (22 kDa, RNA from Selenomonas sp.)
- A protein (20 kDa, Protein from Selenomonas sp.)
- A protein (Complex from Selenomonas sp.)
Cryo-EM structure of Cas8-HNH system at ssDNA-bound state
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8znr
Q-score: 0.505
Li X, Liu Y, Han J, Zhang L, Liu Z, Wang L, Zhang S, Zhang Q, Fu P, Yin H, Zhu H, Zhang H
EMBO J (2024) 43 pp. 4656-4667 [ Pubmed: 39251884 DOI: doi:10.1038/s44318-024-00229-8 ]
- Rna (56-mer) (22 kDa, RNA from Selenomonas sp.)
- Protein structure (39 kDa, Protein from Selenomonas sp.)
- Dna (35-mer) (10 kDa, DNA from Selenomonas sp.)
- Protein complex (Complex from Selenomonas sp.)
- Protein structure (28 kDa, Protein from Selenomonas sp.)
- Protein structure (20 kDa, Protein from Selenomonas sp.)
- Protein structure (37 kDa, Protein from Selenomonas sp.)
Cryo-EM structure of Crt-SPARTA-gRNA-tDNA tetramer (NADase active form)
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 8it1
Q-score: 0.271
Gao X, Shang K, Zhu K, Wang L, Mu Z, Fu X, Yu X, Qin B, Zhu H, Ding W, Cui S
Nature (2024) 625 pp. 822-831 [ Pubmed: 37783228 DOI: doi:10.1038/s41586-023-06665-6 ]
- The tetramer structural of prokaryotic argonaute with dna and rna complex (Complex from Thermoflavifilum thermophilum)
- Rna (5'-r(p*up*gp*ap*gp*gp*up*ap*gp*up*ap*gp*gp*up*up*gp*up*ap*up*ap*gp*u)-3') (6 kDa, RNA from synthetic construct)
- Dna (45-mer) (13 kDa, DNA from synthetic construct)
- Piwi domain-containing protein (58 kDa, Protein from Thermoflavifilum thermophilum)
- Tir domain-containing protein (53 kDa, Protein from Thermoflavifilum thermophilum)
Cryo-EM structure of Crt-SPARTA-gRNA-tDNA monomer
Resolution: 3.27 Å
EM Method: Single-particle
Fitted PDBs: 8isz
Q-score: 0.321
Gao X, Shang K, Zhu K, Wang L, Mu Z, Fu X, Yu X, Qin B, Zhu H, Ding W, Cui S
Nature (2024) 625 pp. 822-831 [ Pubmed: 37783228 DOI: doi:10.1038/s41586-023-06665-6 ]
- The dimer structural of prokaryotic argonaute with dna and rna complex (Complex from Thermoflavifilum thermophilum)
- Rna (5'-r(p*up*gp*ap*gp*gp*up*ap*gp*up*ap*gp*gp*up*up*gp*up*ap*up*ap*gp*u)-3') (6 kDa, RNA from synthetic construct)
- Dna (45-mer) (13 kDa, DNA from synthetic construct)
- Piwi domain-containing protein (58 kDa, Protein from Thermoflavifilum thermophilum)
- Tir domain-containing protein (53 kDa, Protein from Thermoflavifilum thermophilum)
Cryo-EM structure of Crt-SPARTA-gRNA-tDNA dimer (conformation-2)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8it0
Q-score: 0.397
Gao X, Shang K, Zhu K, Wang L, Mu Z, Fu X, Yu X, Qin B, Zhu H, Ding W, Cui S
Nature (2024) 625 pp. 822-831 [ Pubmed: 37783228 DOI: doi:10.1038/s41586-023-06665-6 ]
- The dimer of prokaryotic argonaute system with dna and rna (Complex from Thermoflavifilum thermophilum)
- Rna (5'-r(p*up*gp*ap*gp*gp*up*ap*gp*up*ap*gp*gp*up*up*gp*up*ap*up*ap*gp*u)-3') (6 kDa, RNA from synthetic construct)
- Dna (45-mer) (13 kDa, DNA from synthetic construct)
- Piwi domain-containing protein (58 kDa, Protein from Thermoflavifilum thermophilum)
- Tir domain-containing protein (53 kDa, Protein from Thermoflavifilum thermophilum)
Cryo-EM structure of Crt-SPARTA-gRNA-tDNA dimer (conformation-1)
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 8k9g
Q-score: 0.404
Gao X, Shang K, Zhu K, Wang L, Mu Z, Fu X, Yu X, Qin B, Zhu H, Ding W, Cui S
Nature (2024) 625 pp. 822-831 [ Pubmed: 37783228 DOI: doi:10.1038/s41586-023-06665-6 ]
- The dimer of prokaryotic argonaute system with dna and rna (Complex from Thermoflavifilum thermophilum)
- Rna (5'-r(p*up*gp*ap*gp*gp*up*ap*gp*up*ap*gp*gp*up*up*gp*up*ap*up*ap*gp*u)-3') (6 kDa, RNA from synthetic construct)
- Dna (45-mer) (13 kDa, DNA from synthetic construct)
- Piwi domain-containing protein (58 kDa, Protein from Thermoflavifilum thermophilum)
- Tir domain-containing protein (53 kDa, Protein from Thermoflavifilum thermophilum)
Cryo-EM structure of SaCas9-sgRNA-DNA ternary complex
Du W, Zhu H, Qian J, Xue D, Zheng S, Huang Q
Int J Mol Sci (2023) 24 [ Pubmed: 36674715 DOI: doi:10.3390/ijms24021204 ]
- Single-guide rna (sgrna) (RNA from synthetic construct)
- Target dna strand(ts) (DNA from synthetic construct)
- Sacas9-sgrna-target dna ternary complex (Complex from Staphylococcus aureus)
- Sgrna-target dna (Complex)
- Non-target dna strand (nts) (DNA from synthetic construct)
- Sacas9 (Protein from Staphylococcus aureus)
- Sacas9 (Complex)