Stabilized beta-arrestin 1-V2T subcomplex of a GPCR-G protein-beta-arrestin mega-complex
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6ni2
Q-score: 0.348
Nguyen AH, Thomsen ARB, Cahill 3rd TJ, Huang R, Huang LY, Marcink T, Clarke OB, Heissel S, Masoudi A, Ben-Hail D, Samaan F, Dandey VP, Tan YZ, Hong C, Mahoney JP, Triest S, Little 4th J, Chen X, Sunahara R, Steyaert J, Molina H, Yu Z, des Georges A, Lefkowitz RJ
Nat Struct Mol Biol (2019) 26 pp. 1123-1131 [ DOI: doi:10.1038/s41594-019-0330-y Pubmed: 31740855 ]
- Beta-arrestin-1 (44 kDa, Protein from Bos taurus)
- Fab30 light chain (23 kDa, Protein from synthetic construct)
- Fab30 heavy chain (25 kDa, Protein from synthetic construct)
- Vasopressin v2 receptor (3 kDa, Protein from Homo sapiens)
- Vasopressin 2 tail (Complex from Homo sapiens)
- Nanobody 32 (Complex from Lama glama)
- Nanobody 32 (13 kDa, Protein from Lama glama)
- Fab30 (Complex from synthetic construct)
- Beta-arrestin-1 (Complex from Bos taurus)
- Stabilized beta-arrestin 1-v2t subcomplex of a gpcr-g protein-beta-arrestin mega-complex (Complex)
B2V2R-Gs protein subcomplex of a GPCR-G protein-beta-arrestin mega-complex
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6ni3
Q-score: 0.39
Nguyen AH, Thomsen ARB, Cahill 3rd TJ, Huang R, Huang LY, Marcink T, Clarke OB, Heissel S, Masoudi A, Ben-Hail D, Samaan F, Dandey VP, Tan YZ, Hong C, Mahoney JP, Triest S, Little 4th J, Chen X, Sunahara R, Steyaert J, Molina H, Yu Z, des Georges A, Lefkowitz RJ
Nat Struct Mol Biol (2019) 26 pp. 1123-1131 [ DOI: doi:10.1038/s41594-019-0330-y Pubmed: 31740855 ]
- Nanobody 35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- B2v2r-gs protein subcomplex of a gpcr-g protein-beta-arrestin mega-complex (Complex)
- B2v2r-gs protein subcomplex (Complex from Homo sapiens)
- Endolysin,beta-2 adrenergic receptor chimera (57 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (44 kDa, Protein from Homo sapiens)
- 8-[(1r)-2-{[1,1-dimethyl-2-(2-methylphenyl)ethyl]amino}-1-hydroxyethyl]-5-hydroxy-2h-1,4-benzoxazin-3(4h)-one (370 Da, Ligand)
Structural basis for gating and activation of RyR1 (30 uM Ca2+ dataset, all particles)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 5t15
Q-score: 0.398
des Georges A, Clarke OB, Zalk R, Yuan Q, Condon KJ, Grassucci RA, Hendrickson WA, Marks AR, Frank J
Cell (2016) 167 pp. 145-157.e17 [ Pubmed: 27662087 DOI: doi:10.1016/j.cell.2016.08.075 ]
- Peptidyl-prolyl cis-trans isomerase fkbp1b (11 kDa, Protein from Homo sapiens)
- Ryr1-cs2 complex (Complex)
- Ryanodine receptor 1,ryanodine receptor 1,ryanodine receptor 1,ryanodine receptor 1,ryanodine receptor 1,ryanodine receptor 1,ryanodine receptor 1 (501 kDa, Protein from Oryctolagus cuniculus)
- Zinc ion (65 Da, Ligand)
- Calcium ion (40 Da, Ligand)
Structure of rabbit RyR1 (Ca2+-only dataset, class 1)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 5t9m
Q-score: 0.347
des Georges A, Clarke OB, Zalk R, Yuan Q, Condon KJ, Grassucci RA, Hendrickson WA, Marks AR, Frank J
Cell (2016) 167 pp. 145-157.e17 [ Pubmed: 27662087 DOI: doi:10.1016/j.cell.2016.08.075 ]
- Ryanodine receptor 1 (501 kDa, Protein from Oryctolagus cuniculus)
- Peptidyl-prolyl cis-trans isomerase fkbp1b (11 kDa, Protein from Homo sapiens)
- Ryr1-cs2 complex (Complex)
- Zinc ion (65 Da, Ligand)
- Calcium ion (40 Da, Ligand)
Structure of rabbit RyR1 (Ca2+-only dataset, class 2)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 5t9n
Q-score: 0.368
des Georges A, Clarke OB, Zalk R, Yuan Q, Condon KJ, Grassucci RA, Hendrickson WA, Marks AR, Frank J
Cell (2016) 167 pp. 145-157.e17 [ Pubmed: 27662087 DOI: doi:10.1016/j.cell.2016.08.075 ]
- Ryanodine receptor 1 (501 kDa, Protein from Oryctolagus cuniculus)
- Peptidyl-prolyl cis-trans isomerase fkbp1b (11 kDa, Protein from Homo sapiens)
- Ryr1-cs2 complex (Complex)
- Zinc ion (65 Da, Ligand)
- Calcium ion (40 Da, Ligand)
Structure of rabbit RyR1 (Ca2+-only dataset, class 3)
Resolution: 5.8 Å
EM Method: Single-particle
Fitted PDBs: 5t9r
Q-score: 0.19
des Georges A, Clarke OB, Zalk R, Yuan Q, Condon KJ, Grassucci RA, Hendrickson WA, Marks AR, Frank J
Cell (2016) 167 pp. 145-157.e17 [ Pubmed: 27662087 DOI: doi:10.1016/j.cell.2016.08.075 ]
- Ryanodine receptor 1 (501 kDa, Protein from Oryctolagus cuniculus)
- Peptidyl-prolyl cis-trans isomerase fkbp1b (11 kDa, Protein from Homo sapiens)
- Ryr1-cs2 complex (Complex)
- Zinc ion (65 Da, Ligand)
- Calcium ion (40 Da, Ligand)
Structure of rabbit RyR1 (Ca2+-only dataset, class 4)
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 5t9s
Q-score: 0.287
des Georges A, Clarke OB, Zalk R, Yuan Q, Condon KJ, Grassucci RA, Hendrickson WA, Marks AR, Frank J
Cell (2016) 167 pp. 145-157.e17 [ Pubmed: 27662087 DOI: doi:10.1016/j.cell.2016.08.075 ]
- Ryanodine receptor 1 (501 kDa, Protein from Oryctolagus cuniculus)
- Peptidyl-prolyl cis-trans isomerase fkbp1b (11 kDa, Protein from Homo sapiens)
- Ryr1-cs2 complex (Complex)
- Zinc ion (65 Da, Ligand)
- Calcium ion (40 Da, Ligand)
Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 1)
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 5t9v
Q-score: 0.268
des Georges A, Clarke OB, Zalk R, Yuan Q, Condon KJ, Grassucci RA, Hendrickson WA, Marks AR, Frank J
Cell (2016) 167 pp. 145-157.e17 [ Pubmed: 27662087 DOI: doi:10.1016/j.cell.2016.08.075 ]
- Peptidyl-prolyl cis-trans isomerase fkbp1b (11 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Ryr1-cs2 complex (Complex)
- Ryanodine receptor 1 (475 kDa, Protein from Oryctolagus cuniculus)
- Zinc ion (65 Da, Ligand)
- Caffeine (194 Da, Ligand)
- Calcium ion (40 Da, Ligand)
Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 2)
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 5ta3
Q-score: 0.275
des Georges A, Clarke OB, Zalk R, Yuan Q, Condon KJ, Grassucci RA, Hendrickson WA, Marks AR, Frank J
Cell (2016) 167 pp. 145-157.e17 [ Pubmed: 27662087 DOI: doi:10.1016/j.cell.2016.08.075 ]
- Peptidyl-prolyl cis-trans isomerase fkbp1b (11 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Ryr1-cs2 complex (Complex)
- Ryanodine receptor 1 (475 kDa, Protein from Oryctolagus cuniculus)
- Zinc ion (65 Da, Ligand)
- Caffeine (194 Da, Ligand)
- Calcium ion (40 Da, Ligand)
Structure of rabbit RyR1 (Caffeine/ATP/Ca2+ dataset, class 1 2)
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 5tal
Q-score: 0.303
des Georges A, Clarke OB, Zalk R, Yuan Q, Condon KJ, Grassucci RA, Hendrickson WA, Marks AR, Frank J
Cell (2016) 167 pp. 145-157.e17 [ Pubmed: 27662087 DOI: doi:10.1016/j.cell.2016.08.075 ]
- Peptidyl-prolyl cis-trans isomerase fkbp1b (11 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Ryr1-cs2 complex (Complex)
- Ryanodine receptor 1 (475 kDa, Protein from Oryctolagus cuniculus)
- Zinc ion (65 Da, Ligand)
- Caffeine (194 Da, Ligand)
- Calcium ion (40 Da, Ligand)