S. pombe microtubule decorated with Cut7 motor domain in the AMPPNP state
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 5mlv
Q-score: 0.355
von Loeffelholz O, Pena A, Drummond DR, Cross R, Moores CA
J Mol Biol (2019) 431 pp. 864-872 [ Pubmed: 30659798 DOI: doi:10.1016/j.jmb.2019.01.011 ]
- Magnesium ion (24 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1 chain (51 kDa, Protein from Schizosaccharomyces pombe 972h-)
- 7,11-dihydroxy-8,8,10,12,16-pentamethyl-3-[1-methyl-2-(2-methyl-thiazol-4-yl)vinyl]-4,17-dioxabicyclo[14.1.0]heptadecane-5,9-dione (507 Da, Ligand)
- Cut7 (Complex from Schizosaccharomyces pombe 972)
- Cut7 mtd decorated s. pombe microtubule stabilized with epothilone-b (Complex)
- Kinesin-like protein cut7 (47 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Alpha tubulin, beta tubulin (Complex from Schizosaccharomyces pombe 972)
Volta phase plate data collection facilitates image processing and cryo-EM structure determination
von Loeffelholz O, Papai G, Danev R, Myasnikov AG, Natchiar SK, Hazemann I, Menetret JF, Klaholz BP
J Struct Biol (2018) [ Pubmed: 29337113 DOI: doi:10.1016/j.jsb.2018.01.003 ]
- Ribosome (Complex from Homo sapiens)
Volta phase plate data collection facilitates image processing and cryo-EM structure determination
von Loeffelholz O, Papai G, Danev R, Myasnikov AG, Natchiar SK, Hazemann I, Menetret JF, Klaholz BP
J Struct Biol (2018) [ Pubmed: 29337113 DOI: doi:10.1016/j.jsb.2018.01.003 ]
- Ribosome (Complex from Homo sapiens)
Volta phase plate data collection facilitates image processing and cryo-EM structure determination
von Loeffelholz O, Papai G, Danev R, Myasnikov AG, Natchiar SK, Hazemann I, Menetret JF, Klaholz BP
J Struct Biol (2018) 202 pp. 191-199 [ Pubmed: 29337113 DOI: doi:10.1016/j.jsb.2018.01.003 ]
- Ribosome (Complex from Homo sapiens)
S. pombe microtubule copolymerized with GTP and Mal3-143
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 5mjs
Q-score: 0.331
von Loeffelholz O, Venables NA, Drummond DR, Katsuki M, Cross R, Moores CA
Nat Commun (2017) 8 pp. 2110-2110 [ DOI: doi:10.1038/s41467-017-02241-5 Pubmed: 29235477 ]
- Tubulin beta chain (47 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Tubulin alpha-1 chain (49 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Guanosine-5'-diphosphate (443 Da, Ligand from Schizosaccharomyces pombe)
- Microtubule integrity protein mal3 (16 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Guanosine-5'-triphosphate (523 Da, Ligand from Schizosaccharomyces pombe)
- Microtubule decorated with mal3 (Complex from Schizosaccharomyces pombe)