The closed state of the ASFV apo-RNA polymerase
Resolution: 2.69 Å
EM Method: Single-particle
Fitted PDBs: 8q3b
Q-score: 0.54
Pilotto S, Sykora M, Cackett G, Dulson C, Werner F
Nat Commun (2024) 15 pp. 1606-1606 [ DOI: doi:10.1038/s41467-024-45842-7 Pubmed: 38383525 ]
- Dna-directed rna polymerase rpb6 homolog (16 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb7 homolog (38 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb10 homolog (9 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb5 homolog (23 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb2 homolog (140 kDa, Protein from African swine fever virus BA71V)
- Magnesium ion (24 Da, Ligand)
- Uncharacterized protein c122r (11 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb3-11 homolog (41 kDa, Protein from African swine fever virus BA71V)
- Apo-form of the 8-subunit rna polymerase from african swine fever virus (450 kDa, Complex from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb1 homolog (163 kDa, Protein from African swine fever virus BA71V)
- Zinc ion (65 Da, Ligand)
The open state of the ASFV apo-RNA polymerase
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 8q3k
Q-score: 0.531
Pilotto S, Sykora M, Cackett G, Dulson C, Werner F
Nat Commun (2024) 15 pp. 1606-1606 [ DOI: doi:10.1038/s41467-024-45842-7 Pubmed: 38383525 ]
- Dna-directed rna polymerase rpb6 homolog (16 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb7 homolog (38 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb10 homolog (9 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb5 homolog (23 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb2 homolog (140 kDa, Protein from African swine fever virus BA71V)
- Magnesium ion (24 Da, Ligand)
- Uncharacterized protein c122r (11 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb3-11 homolog (41 kDa, Protein from African swine fever virus BA71V)
- Apo-form of the 8-subunit rna polymerase from african swine fever virus (450 kDa, Complex from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb1 homolog (163 kDa, Protein from African swine fever virus BA71V)
- Zinc ion (65 Da, Ligand)
consensus map of the ASFV RNA polymerase in open conformation
The stalk domain of the ASFV RNA polymerase obtained from Multibody refinement
Structure of Full-Length AsfvPrimPol in Apo-Form
Resolution: 3.67 Å
EM Method: Single-particle
Fitted PDBs: 8iqh
Q-score: 0.383
Shao Z, Su S, Yang J, Zhang W, Gao Y, Zhao X, Zhang Y, Shao Q, Cao C, Li H, Liu H, Zhang J, Lin J, Ma J, Gan J
Nucleic Acids Res (2023) 51 pp. 9475-9490 [ Pubmed: 37587714 DOI: doi:10.1093/nar/gkad677 ]
- Putative primase c962r (111 kDa, Protein from African swine fever virus BA71V)
- Full-length asfvprimpol alone (1 MDa, Complex from African swine fever virus BA71V)
Structure of Full-Length AsfvPrimPol in Complex-Form
Resolution: 3.32 Å
EM Method: Single-particle
Fitted PDBs: 8iqi
Q-score: 0.393
Shao Z, Su S, Yang J, Zhang W, Gao Y, Zhao X, Zhang Y, Shao Q, Cao C, Li H, Liu H, Zhang J, Lin J, Ma J, Gan J
Nucleic Acids Res (2023) 51 pp. 9475-9490 [ Pubmed: 37587714 DOI: doi:10.1093/nar/gkad677 ]
- Full-length asfvprimpol (Complex from African swine fever virus BA71V)
- Dna (32-mer) (9 kDa, DNA from synthetic construct)
- Full-length asfvprimpol in complex with dna and amppnp (660 kDa, Complex)
- Putative primase c962r (111 kDa, Protein from African swine fever virus BA71V)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex)
Structure of African Swine fever virus
Andres G, Charro D, Matamoros T, Dillard RS, Abrescia NGA
J Biol Chem (2019) [ DOI: doi:10.1074/jbc.AC119.011196 Pubmed: 31649031 ]