The closed state of the ASFV apo-RNA polymerase
Resolution: 2.69 Å
EM Method: Single-particle
Fitted PDBs: 8q3b
Q-score: 0.54
Pilotto S, Sykora M, Cackett G, Dulson C, Werner F
Nat Commun (2024) 15 pp. 1606-1606 [ DOI: doi:10.1038/s41467-024-45842-7 Pubmed: 38383525 ]
- Dna-directed rna polymerase rpb5 homolog (23 kDa, Protein from African swine fever virus BA71V)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase rpb2 homolog (140 kDa, Protein from African swine fever virus BA71V)
- Uncharacterized protein c122r (11 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb3-11 homolog (41 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb7 homolog (38 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb1 homolog (163 kDa, Protein from African swine fever virus BA71V)
- Magnesium ion (24 Da, Ligand)
- Apo-form of the 8-subunit rna polymerase from african swine fever virus (450 kDa, Complex from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb6 homolog (16 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb10 homolog (9 kDa, Protein from African swine fever virus BA71V)
The open state of the ASFV apo-RNA polymerase
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 8q3k
Q-score: 0.531
Pilotto S, Sykora M, Cackett G, Dulson C, Werner F
Nat Commun (2024) 15 pp. 1606-1606 [ DOI: doi:10.1038/s41467-024-45842-7 Pubmed: 38383525 ]
- Dna-directed rna polymerase rpb5 homolog (23 kDa, Protein from African swine fever virus BA71V)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase rpb2 homolog (140 kDa, Protein from African swine fever virus BA71V)
- Uncharacterized protein c122r (11 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb3-11 homolog (41 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb7 homolog (38 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb1 homolog (163 kDa, Protein from African swine fever virus BA71V)
- Magnesium ion (24 Da, Ligand)
- Apo-form of the 8-subunit rna polymerase from african swine fever virus (450 kDa, Complex from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb6 homolog (16 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb10 homolog (9 kDa, Protein from African swine fever virus BA71V)
consensus map of the ASFV RNA polymerase in open conformation
The stalk domain of the ASFV RNA polymerase obtained from Multibody refinement
Structure of Full-Length AsfvPrimPol in Apo-Form
Resolution: 3.67 Å
EM Method: Single-particle
Fitted PDBs: 8iqh
Q-score: 0.383
Shao Z, Su S, Yang J, Zhang W, Gao Y, Zhao X, Zhang Y, Shao Q, Cao C, Li H, Liu H, Zhang J, Lin J, Ma J, Gan J
Nucleic Acids Res (2023) 51 pp. 9475-9490 [ Pubmed: 37587714 DOI: doi:10.1093/nar/gkad677 ]
- Putative primase c962r (111 kDa, Protein from African swine fever virus BA71V)
- Full-length asfvprimpol alone (1 MDa, Complex from African swine fever virus BA71V)
Structure of Full-Length AsfvPrimPol in Complex-Form
Resolution: 3.32 Å
EM Method: Single-particle
Fitted PDBs: 8iqi
Q-score: 0.393
Shao Z, Su S, Yang J, Zhang W, Gao Y, Zhao X, Zhang Y, Shao Q, Cao C, Li H, Liu H, Zhang J, Lin J, Ma J, Gan J
Nucleic Acids Res (2023) 51 pp. 9475-9490 [ Pubmed: 37587714 DOI: doi:10.1093/nar/gkad677 ]
- Dna (Complex)
- Putative primase c962r (111 kDa, Protein from African swine fever virus BA71V)
- Full-length asfvprimpol in complex with dna and amppnp (660 kDa, Complex)
- Full-length asfvprimpol (Complex from African swine fever virus BA71V)
- Dna (32-mer) (9 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
Structure of African Swine fever virus
Andres G, Charro D, Matamoros T, Dillard RS, Abrescia NGA
J Biol Chem (2019) [ DOI: doi:10.1074/jbc.AC119.011196 Pubmed: 31649031 ]