The closed state of the ASFV apo-RNA polymerase
Resolution: 2.69 Å
EM Method: Single-particle
Fitted PDBs: 8q3b
Q-score: 0.54
Pilotto S, Sykora M, Cackett G, Dulson C, Werner F
Nat Commun (2024) 15 pp. 1606-1606 [ Pubmed: 38383525 DOI: doi:10.1038/s41467-024-45842-7 ]
- Dna-directed rna polymerase rpb10 homolog (9 kDa, Protein from African swine fever virus BA71V)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase rpb6 homolog (16 kDa, Protein from African swine fever virus BA71V)
- Uncharacterized protein c122r (11 kDa, Protein from African swine fever virus BA71V)
- Apo-form of the 8-subunit rna polymerase from african swine fever virus (450 kDa, Complex from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb5 homolog (23 kDa, Protein from African swine fever virus BA71V)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase rpb1 homolog (163 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb2 homolog (140 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb3-11 homolog (41 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb7 homolog (38 kDa, Protein from African swine fever virus BA71V)
The open state of the ASFV apo-RNA polymerase
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 8q3k
Q-score: 0.531
Pilotto S, Sykora M, Cackett G, Dulson C, Werner F
Nat Commun (2024) 15 pp. 1606-1606 [ Pubmed: 38383525 DOI: doi:10.1038/s41467-024-45842-7 ]
- Dna-directed rna polymerase rpb10 homolog (9 kDa, Protein from African swine fever virus BA71V)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase rpb6 homolog (16 kDa, Protein from African swine fever virus BA71V)
- Uncharacterized protein c122r (11 kDa, Protein from African swine fever virus BA71V)
- Apo-form of the 8-subunit rna polymerase from african swine fever virus (450 kDa, Complex from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb5 homolog (23 kDa, Protein from African swine fever virus BA71V)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase rpb1 homolog (163 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb2 homolog (140 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb3-11 homolog (41 kDa, Protein from African swine fever virus BA71V)
- Dna-directed rna polymerase rpb7 homolog (38 kDa, Protein from African swine fever virus BA71V)
consensus map of the ASFV RNA polymerase in open conformation
The stalk domain of the ASFV RNA polymerase obtained from Multibody refinement
Structure of African Swine fever virus
Andres G, Charro D, Matamoros T, Dillard RS, Abrescia NGA
J Biol Chem (2019) [ Pubmed: 31649031 DOI: doi:10.1074/jbc.AC119.011196 ]