Cas12a:gRNA:DNA (Acidaminococcus sp.) with 0 RNA:DNA base pairs, structure 1
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 9cjh
Q-score: 0.296
Soczek KM, Cofsky JC, Tuck OT, Shi H, Doudna JA
Nucleic Acids Res (2025) 53 [ Pubmed: 39698811 DOI: doi:10.1093/nar/gkae1192 ]
- Cas12a (Complex from Acidaminococcus sp. BV3L6)
- Grna:dna (Complex from synthetic construct)
- Non-target dna strand (9 kDa, DNA from synthetic construct)
- Crispr-associated endonuclease cas12a (151 kDa, Protein from Acidaminococcus sp. BV3L6)
- Cas12a:grna:dna (acidaminococcus sp.) with 0 rna:dna base pairs, structure 1 (185 kDa, Complex)
- Guide rna (25-mer) (13 kDa, RNA from synthetic construct)
- Target dna strand (9 kDa, DNA from synthetic construct)
Cas12a:gRNA:DNA (Acidaminococcus sp.) with 0 RNA:DNA base pairs, structure 2
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 9cji
Q-score: 0.316
Soczek KM, Cofsky JC, Tuck OT, Shi H, Doudna JA
Nucleic Acids Res (2025) 53 [ Pubmed: 39698811 DOI: doi:10.1093/nar/gkae1192 ]
- Cas12a (Complex from Acidaminococcus sp. BV3L6)
- Grna:dna (Complex from synthetic construct)
- Non-target dna strand (9 kDa, DNA from synthetic construct)
- Cas12a:grna:dna (acidaminococcus sp.) with 0 rna:dna base pairs, structure 2 (185 kDa, Complex)
- Crispr-associated endonuclease cas12a (151 kDa, Protein from Acidaminococcus sp. BV3L6)
- Guide rna (25-mer) (13 kDa, RNA from synthetic construct)
- Target dna strand (9 kDa, DNA from synthetic construct)
Cas12a:gRNA:DNA (Acidaminococcus sp.) with 0 RNA:DNA base pairs, structure 3
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 9cjj
Q-score: 0.324
Soczek KM, Cofsky JC, Tuck OT, Shi H, Doudna JA
Nucleic Acids Res (2024) [ Pubmed: 39698811 DOI: doi:10.1093/nar/gkae1192 ]
- Cas12a (Complex from Acidaminococcus sp. BV3L6)
- Grna:dna (Complex from synthetic construct)
- Non-target dna strand (9 kDa, DNA from synthetic construct)
- Crispr-associated endonuclease cas12a (151 kDa, Protein from Acidaminococcus sp. BV3L6)
- Guide rna (25-mer) (13 kDa, RNA from synthetic construct)
- Cas12a:grna:dna (acidaminococcus sp.) with 0 rna:dna base pairs, structure 3 (185 kDa, Complex)
- Target dna strand (9 kDa, DNA from synthetic construct)
WT CRISPR-Cas12a with a 5bp R-loop
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8sfh
Q-score: 0.397
Strohkendl I, Saha A, Moy C, Nguyen AH, Ahsan M, Russell R, Palermo G, Taylor DW
Mol Cell (2024) 84 pp. 2717-2731.e6 [ DOI: doi:10.1016/j.molcel.2024.06.007 Pubmed: 38955179 ]
- Wt ascas12a incubated with 8bp-complementary target dna. (Complex from Acidaminococcus sp. BV3L6)
- Crispr-associated endonuclease cas12a (151 kDa, Protein from Acidaminococcus sp. BV3L6)
- Dna (32-mer) (9 kDa, DNA from synthetic construct)
- Dna (32-mer) (9 kDa, DNA from synthetic construct)
- Rna (27-mer) (8 kDa, RNA from synthetic construct)
WT CRISPR-Cas12a with a 8bp R-loop
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8sfi
Q-score: 0.36
Strohkendl I, Saha A, Moy C, Nguyen AH, Ahsan M, Russell R, Palermo G, Taylor DW
Mol Cell (2024) 84 pp. 2717-2731.e6 [ DOI: doi:10.1016/j.molcel.2024.06.007 Pubmed: 38955179 ]
- Dna (5'-d(p*cp*tp*tp*ap*tp*cp*ap*cp*tp*ap*ap*ap*ap*gp*ap*tp*cp*gp*gp*ap*ap*g)-3') (17 kDa, DNA from synthetic construct)
- Crispr-associated endonuclease cas12a (151 kDa, Protein from Acidaminococcus sp. BV3L6)
- Dna (5'-d(p*cp*tp*tp*cp*cp*gp*ap*tp*cp*tp*tp*tp*tp*ap*gp*tp*gp*ap*tp*a)-3') (17 kDa, DNA from synthetic construct)
- Rna (28-mer) (15 kDa, RNA from synthetic construct)
- Wt ascas12a incubated with 8bp-complementary target dna (Complex from Acidaminococcus sp. BV3L6)
WT CRISPR-Cas12a with a 10bp R-loop
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8sfj
Q-score: 0.346
Strohkendl I, Saha A, Moy C, Nguyen AH, Ahsan M, Russell R, Palermo G, Taylor DW
Mol Cell (2024) 84 pp. 2717-2731.e6 [ DOI: doi:10.1016/j.molcel.2024.06.007 Pubmed: 38955179 ]
- Crispr-associated endonuclease cas12a (151 kDa, Protein from Acidaminococcus sp. BV3L6)
- Dna (5'-d(p*cp*ap*cp*tp*tp*ap*tp*cp*ap*cp*tp*ap*ap*ap*ap*gp*ap*tp*cp*gp*gp*ap*ap*g)-3') (17 kDa, DNA from synthetic construct)
- Wt ascas12a incubated with 12bp-complementary target dna (Complex from Acidaminococcus sp. BV3L6)
- Dna (5'-d(p*cp*tp*tp*cp*cp*gp*ap*tp*cp*tp*tp*tp*tp*ap*gp*tp*gp*ap*t)-3') (17 kDa, DNA from synthetic construct)
- Rna (29-mer) (15 kDa, RNA from synthetic construct)
WT CRISPR-Cas12a with a 15bp R-loop
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8sfl
Q-score: 0.381
Strohkendl I, Saha A, Moy C, Nguyen AH, Ahsan M, Russell R, Palermo G, Taylor DW
Mol Cell (2024) 84 pp. 2717-2731.e6 [ DOI: doi:10.1016/j.molcel.2024.06.007 Pubmed: 38955179 ]
- Crispr-associated endonuclease cas12a (151 kDa, Protein from Acidaminococcus sp. BV3L6)
- Dna (40-mer) (17 kDa, DNA from synthetic construct)
- Dna (40-mer) (17 kDa, DNA from synthetic construct)
- Wt ascas12a incubated with 16bp-complementary target dna (Complex from Acidaminococcus sp. BV3L6)
- Rna (34-mer) (15 kDa, RNA from synthetic construct)
WT CRISPR-Cas12a with a 16bp R-loop and nontarget strand in the RuvC active site.
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8sfn
Q-score: 0.332
Strohkendl I, Saha A, Moy C, Nguyen AH, Ahsan M, Russell R, Palermo G, Taylor DW
Mol Cell (2024) 84 pp. 2717-2731.e6 [ DOI: doi:10.1016/j.molcel.2024.06.007 Pubmed: 38955179 ]
- Crispr-associated endonuclease cas12a (151 kDa, Protein from Acidaminococcus sp. BV3L6)
- Rna (36-mer) (15 kDa, RNA from synthetic construct)
- Dna (31-mer) (17 kDa, DNA from synthetic construct)
- Dna (30-mer) (17 kDa, DNA from synthetic construct)
- Wt ascas12a incubated with 16bp-complementary target dna. (Complex from Acidaminococcus sp. BV3L6)
WT CRISPR-Cas12a with a 20bp R-loop and nontarget strand in the RuvC active site.
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8sfo
Q-score: 0.397
Strohkendl I, Saha A, Moy C, Nguyen AH, Ahsan M, Russell R, Palermo G, Taylor DW
Mol Cell (2024) 84 pp. 2717-2731.e6 [ DOI: doi:10.1016/j.molcel.2024.06.007 Pubmed: 38955179 ]
- Dna (35-mer) (17 kDa, DNA from synthetic construct)
- Crispr-associated endonuclease cas12a (151 kDa, Protein from Acidaminococcus sp. BV3L6)
- Dna (40-mer) (17 kDa, DNA from synthetic construct)
- Wt ascas12a incubated with 20bp-complementary target dna. (Complex from Acidaminococcus sp. BV3L6)
- Rna (39-mer) (15 kDa, RNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
WT CRISPR-Cas12a with the target strand in the RuvC active site.
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8sfp
Q-score: 0.321
Strohkendl I, Saha A, Moy C, Nguyen AH, Ahsan M, Russell R, Palermo G, Taylor DW
Mol Cell (2024) 84 pp. 2717-2731.e6 [ DOI: doi:10.1016/j.molcel.2024.06.007 Pubmed: 38955179 ]
- Crispr-associated endonuclease cas12a (151 kDa, Protein from Acidaminococcus sp. BV3L6)
- Dna (40-mer) (17 kDa, DNA from synthetic construct)
- Rna (39-mer) (15 kDa, RNA from synthetic construct)
- Dna (5'-d(p*cp*tp*tp*cp*cp*gp*ap*tp*cp*tp*tp*tp*tp*ap*gp*tp*gp*ap*tp*a)-3') (17 kDa, DNA from synthetic construct)
- Wt ascas12a incubated with 20bp-complementary target dna with phosphorothioate-modified target strand. (Complex from Acidaminococcus sp. BV3L6)