Monomeric Lassa glycoprotein bound to 25.10C Fab and two interior-binding rabbit polyclonal Fabs
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8vcv
Q-score: 0.579
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
- Glycoprotein g2 (44 kDa, Protein from Lassa virus Josiah)
- Rabbit polyclonal fv heavy chain (10 kDa, Protein from Oryctolagus cuniculus)
- Lineage iv lassa virus glycoprotein (josiah) in complex with rabbit polyclonal antibody (gpc-c epitope) (Complex from Oryctolagus cuniculus)
- Rabbit polyclonal fv light chain (9 kDa, Protein from Oryctolagus cuniculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus Josiah)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8ve8
Q-score: 0.599
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
- Rabbit polyclonal fv light chain (9 kDa, Protein from Oryctolagus cuniculus)
- Glycoprotein g2 (44 kDa, Protein from Lassa virus Josiah)
- Rabbit polyclonal fv heavy chain (10 kDa, Protein from Oryctolagus cuniculus)
- Lineage iv lassa virus glycoprotein (josiah) in complex with rabbit polyclonal antibody (gp1-a epitope) (Complex from Oryctolagus cuniculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus Josiah)
Lineage IV Lassa virus glycoprotein (Josiah) in complex with monoclonal antibody 8.9F
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 9cj7
Q-score: 0.512
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Fv region of 8.9f light chain (25 kDa, Protein from Homo sapiens)
- Fv region of 8.9f heavy chain (31 kDa, Protein from Homo sapiens)
- Glycoprotein g2 (46 kDa, Protein from Lassa virus Josiah)
- Lineage iv lassa virus glycoprotein (josiah) in complex with monoclonal antibody 8.9f (Complex from Oryctolagus cuniculus)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus Josiah)
Resolution: 3.74 Å
EM Method: Single-particle
Fitted PDBs: 9cj8
Q-score: 0.423
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
- Glycoprotein g2 (44 kDa, Protein from Lassa virus Josiah)
- Lineage iv lassa virus glycoprotein (josiah) in complex with rabbit polyclonal antibody (ll-1 epitope) (Complex from Oryctolagus cuniculus)
- Rabbit polyclonal fv heavy chain (9 kDa, Protein from Oryctolagus cuniculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Rabbit polyclonal fv light chain (8 kDa, Protein from Oryctolagus cuniculus)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus Josiah)
Resolution: 3.45 Å
EM Method: Single-particle
Fitted PDBs: 9ck7
Q-score: 0.454
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
- Glycoprotein gp1 (29 kDa, Protein from Lassa virus Josiah)
- Rabbit polyclonal fv heavy chain (10 kDa, Protein from Oryctolagus cuniculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Rabbit polyclonal fv light chain (9 kDa, Protein from Oryctolagus cuniculus)
- Glycoprotein g2 (46 kDa, Protein from Lassa virus Josiah)
- Lineage iv lassa virus glycoprotein (josiah) in complex with rabbit polyclonal antibody (gpc-a-1 epitope) (Complex from Oryctolagus cuniculus)
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 9ck8
Q-score: 0.528
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
- Rabbit polyclonal fv heavy chain (10 kDa, Protein from Oryctolagus cuniculus)
- Glycoprotein gp1 (29 kDa, Protein from Lassa virus Josiah)
- Rabbit polyclonal fv light chain (9 kDa, Protein from Oryctolagus cuniculus)
- Lineage iv lassa virus glycoprotein (josiah) in complex with rabbit polyclonal antibody (gpc-a-2 epitope) (Complex from Oryctolagus cuniculus)
- Glycoprotein g2 (46 kDa, Protein from Lassa virus Josiah)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Lassa GPC (strain Josiah) bound to rabbit polyclonal base-targeting antibody Base-1
Brouwer PJM, Perrett HR, Nijhuis H, Lee W-H, Burger J, Lloyd NM, Himansu S, Beaumont T, Carfi A, Bukreyev A, Sanders RW, van Gils MJ, Ward AB
To Be Published
- Heterotrimeric lassa gpc bound to heterodimeric rabbit polyclonal base-targeting antibody base-1 (Complex)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus)
- Glycoprotein g2, 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase fusion protein (44 kDa, Protein from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8))
- Polyclonal antibody base-1 light chain (9 kDa, Protein from Oryctolagus cuniculus)
- Polyclonal antibody base-1 heavy chain, polyclonal antibody base-1 light chain (Complex from Oryctolagus cuniculus)
- Polyclonal antibody base-1 heavy chain (9 kDa, Protein from Oryctolagus cuniculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Glycoprotein g1, glycoprotein g2, 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase fusion protein (Complex from Lassa virus)
Lassa GPC (strain Josiah) bound to rabbit polyclonal fusion-peptide-targeting antibody FP-1
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8tye
Q-score: 0.421
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
- Glycoprotein g1, glycoprotein g2, 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase fusion protein (Complex from Lassa virus Josiah)
- Polyclonal antibody fp-1 heavy chain, polyclonal antibody fp-1 light chain (Complex from Oryctolagus cuniculus)
- Polyclonal fv heavy chain (9 kDa, Protein from Oryctolagus cuniculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Glycoprotein gp1 (29 kDa, Protein from Lassa virus (strain Mouse/Sierra Leone/Josiah/1976))
- Heterotrimeric lassa gpc bound to heterodimeric rabbit polyclonal fusion-peptide-targeting antibody fp-1 (Complex)
- Glycoprotein gp2 (44 kDa, Protein from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8))
- Polyclonal fv light chain (8 kDa, Protein from Oryctolagus cuniculus)