Lineage IV Lassa virus glycoprotein (Josiah) in complex with monoclonal antibody 8.9F
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 9cj7
Q-score: 0.512
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Fv region of 8.9f light chain (25 kDa, Protein from Homo sapiens)
- Fv region of 8.9f heavy chain (31 kDa, Protein from Homo sapiens)
- Glycoprotein g2 (46 kDa, Protein from Lassa virus Josiah)
- Lineage iv lassa virus glycoprotein (josiah) in complex with monoclonal antibody 8.9f (Complex from Oryctolagus cuniculus)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus Josiah)
Lassa GPC Trimer in complex with Fab 8.11G and nanobody D5
Resolution: 4.7 Å
EM Method: Single-particle
Fitted PDBs: 8t5c
Q-score: 0.301
Gorman J, Cheung CS, Duan Z, Ou L, Wang M, Chen X, Cheng C, Biju A, Sun Y, Wang P, Yang Y, Zhang B, Boyington JC, Bylund T, Charaf S, Chen SJ, Du H, Henry AR, Liu T, Sarfo EK, Schramm CA, Shen CH, Stephens T, Teng IT, Todd JP, Tsybovsky Y, Verardi R, Wang D, Wang S, Wang Z, Zheng CY, Zhou T, Douek DC, Mascola JR, Ho DD, Ho M, Kwong PD
Nat Commun (2024) 15 pp. 285-285 [ DOI: doi:10.1038/s41467-023-44534-y Pubmed: 38177144 ]
- 8.11g light chain (12 kDa, Protein from Homo sapiens)
- Glycoprotein g2 (22 kDa, Protein from Lassa virus Josiah)
- Lassa gpc trimer in complex with fab 8.11g and nanobody d5 (Complex from Lassa virus Josiah)
- 8.11g heavy chain (13 kDa, Protein from Homo sapiens)
- Glycoprotein g1 (22 kDa, Protein from Lassa virus Josiah)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- D5 nanobody (13 kDa, Protein from Camelus bactrianus)
Native Lassa glycoprotein in complex with neutralizing antibodies 8.9F and 37.2D
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 7uot
Q-score: 0.559
Li H, Buck T, Zandonatti M, Yin J, Moon-Walker A, Fang J, Koval A, Heinrich ML, Rowland MM, Diaz Avalos R, Schendel SL, Parekh D, Zyla D, Enriquez A, Harkins S, Sullivan B, Smith V, Chukwudozie O, Watanabe R, Robinson JE, Garry RF, Branco LM, Hastie KM, Saphire EO
Sci Transl Med (2022) 14 pp. eabq0991-eabq0991 [ DOI: doi:10.1126/scitranslmed.abq0991 Pubmed: 36288283 ]
- 37.2d light chain (variable domain) (11 kDa, Protein from Homo sapiens)
- 37.2d heavy chain (variable domain) (13 kDa, Protein from Homo sapiens)
- Glycoprotein g2 (26 kDa, Protein from Lassa virus)
- Native lassa glycoprotein in complex with 8.9f-scfv and 37.2d-scfv (Complex)
- 8.9f heavy chain (variable domain) (15 kDa, Protein from Homo sapiens)
- 8.9f light chain (variable domain) (11 kDa, Protein from Homo sapiens)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus)
Native Lassa glycoprotein in complex with neutralizing antibodies 12.1F and 37.2D
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 7uov
Q-score: 0.541
Li H, Buck T, Zandonatti M, Yin J, Moon-Walker A, Fang J, Koval A, Heinrich ML, Rowland MM, Diaz Avalos R, Schendel SL, Parekh D, Zyla D, Enriquez A, Harkins S, Sullivan B, Smith V, Chukwudozie O, Watanabe R, Robinson JE, Garry RF, Branco LM, Hastie KM, Saphire EO
Sci Transl Med (2022) 14 pp. eabq0991-eabq0991 [ DOI: doi:10.1126/scitranslmed.abq0991 Pubmed: 36288283 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Native lassa glycoprotein in complex with 12.1f-scfv and 37.2d-scfv (Complex)
- 37.2d light chain (variable domain) (11 kDa, Protein from Homo sapiens)
- 37.2d heavy chain (variable domain) (13 kDa, Protein from Homo sapiens)
- Glycoprotein g2 (26 kDa, Protein from Lassa virus)
- 12.1f light chain (variable domain) (11 kDa, Protein from Homo sapiens)
- 12.1f heavy chain (variable domain) (12 kDa, Protein from Homo sapiens)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus)
Structure of Lassa Virus glycoprotein (Josiah) bound to Fab 25.10C
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7tyv
Q-score: 0.447
Enriquez AS, Buck TK, Li H, Norris MJ, Moon-Walker A, Zandonatti MA, Harkins SS, Robinson JE, Branco LM, Garry RF, Saphire EO, Hastie KM
Cell Rep (2022) 39 pp. 110841-110841 [ Pubmed: 35613585 DOI: doi:10.1016/j.celrep.2022.110841 ]
- 25.10c fab heavy chain (24 kDa, Protein from Homo sapiens)
- Lassa virus josiah gp complexed with anti-lassa virus antibody fab fragment 25.10c (350 kDa, Complex)
- Glycoprotein g2 (19 kDa, Protein from Lassa virus)
- 25.10c fab light chain (25 kDa, Protein from Homo sapiens)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus)
- Lassa virus glycoprotein josiah (Complex from Lassa virus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 25.10c fab fragment (Complex from Homo sapiens)