Monomeric Lassa glycoprotein bound to 25.10C Fab and two interior-binding rabbit polyclonal Fabs
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8vcv
Q-score: 0.579
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
- Rabbit polyclonal fv light chain (9 kDa, Protein from Oryctolagus cuniculus)
- Lineage iv lassa virus glycoprotein (josiah) in complex with rabbit polyclonal antibody (gpc-c epitope) (Complex from Oryctolagus cuniculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus Josiah)
- Rabbit polyclonal fv heavy chain (10 kDa, Protein from Oryctolagus cuniculus)
- Glycoprotein g2 (44 kDa, Protein from Lassa virus Josiah)
Lineage IV Lassa virus glycoprotein (Josiah) in complex with monoclonal antibody 8.9F
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 9cj7
Q-score: 0.512
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
- Glycoprotein g2 (46 kDa, Protein from Lassa virus Josiah)
- Fv region of 8.9f heavy chain (31 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Fv region of 8.9f light chain (25 kDa, Protein from Homo sapiens)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus Josiah)
- Lineage iv lassa virus glycoprotein (josiah) in complex with monoclonal antibody 8.9f (Complex from Oryctolagus cuniculus)
Resolution: 3.74 Å
EM Method: Single-particle
Fitted PDBs: 9cj8
Q-score: 0.423
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
- Lineage iv lassa virus glycoprotein (josiah) in complex with rabbit polyclonal antibody (ll-1 epitope) (Complex from Oryctolagus cuniculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Rabbit polyclonal fv heavy chain (9 kDa, Protein from Oryctolagus cuniculus)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus Josiah)
- Rabbit polyclonal fv light chain (8 kDa, Protein from Oryctolagus cuniculus)
- Glycoprotein g2 (44 kDa, Protein from Lassa virus Josiah)
Resolution: 3.45 Å
EM Method: Single-particle
Fitted PDBs: 9ck7
Q-score: 0.454
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
- Glycoprotein gp1 (29 kDa, Protein from Lassa virus Josiah)
- Glycoprotein g2 (46 kDa, Protein from Lassa virus Josiah)
- Rabbit polyclonal fv light chain (9 kDa, Protein from Oryctolagus cuniculus)
- Lineage iv lassa virus glycoprotein (josiah) in complex with rabbit polyclonal antibody (gpc-a-1 epitope) (Complex from Oryctolagus cuniculus)
- Rabbit polyclonal fv heavy chain (10 kDa, Protein from Oryctolagus cuniculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 9ck8
Q-score: 0.528
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
- Rabbit polyclonal fv heavy chain (10 kDa, Protein from Oryctolagus cuniculus)
- Lineage iv lassa virus glycoprotein (josiah) in complex with rabbit polyclonal antibody (gpc-a-2 epitope) (Complex from Oryctolagus cuniculus)
- Glycoprotein g2 (46 kDa, Protein from Lassa virus Josiah)
- Glycoprotein gp1 (29 kDa, Protein from Lassa virus Josiah)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Rabbit polyclonal fv light chain (9 kDa, Protein from Oryctolagus cuniculus)
Lassa GPC (strain Josiah) bound to rabbit polyclonal base-targeting antibody Base-1
Brouwer PJM, Perrett HR, Nijhuis H, Lee W-H, Burger J, Lloyd NM, Himansu S, Beaumont T, Carfi A, Bukreyev A, Sanders RW, van Gils MJ, Ward AB
To Be Published
- Glycoprotein g1 (29 kDa, Protein from Lassa virus)
- Glycoprotein g2, 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase fusion protein (44 kDa, Protein from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8))
- Polyclonal antibody base-1 heavy chain (9 kDa, Protein from Oryctolagus cuniculus)
- Polyclonal antibody base-1 light chain (9 kDa, Protein from Oryctolagus cuniculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Polyclonal antibody base-1 heavy chain, polyclonal antibody base-1 light chain (Complex from Oryctolagus cuniculus)
- Heterotrimeric lassa gpc bound to heterodimeric rabbit polyclonal base-targeting antibody base-1 (Complex)
- Glycoprotein g1, glycoprotein g2, 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase fusion protein (Complex from Lassa virus)
Lassa GPC (strain Josiah) bound to rabbit polyclonal fusion-peptide-targeting antibody FP-1
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8tye
Q-score: 0.421
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
- Polyclonal fv light chain (8 kDa, Protein from Oryctolagus cuniculus)
- Polyclonal fv heavy chain (9 kDa, Protein from Oryctolagus cuniculus)
- Heterotrimeric lassa gpc bound to heterodimeric rabbit polyclonal fusion-peptide-targeting antibody fp-1 (Complex)
- Glycoprotein gp2 (44 kDa, Protein from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8))
- Polyclonal antibody fp-1 heavy chain, polyclonal antibody fp-1 light chain (Complex from Oryctolagus cuniculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Glycoprotein gp1 (29 kDa, Protein from Lassa virus (strain Mouse/Sierra Leone/Josiah/1976))
- Glycoprotein g1, glycoprotein g2, 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase fusion protein (Complex from Lassa virus Josiah)
Lassa GPC Trimer in complex with Fab 8.11G and nanobody D5
Resolution: 4.7 Å
EM Method: Single-particle
Fitted PDBs: 8t5c
Q-score: 0.301
Gorman J, Cheung CS, Duan Z, Ou L, Wang M, Chen X, Cheng C, Biju A, Sun Y, Wang P, Yang Y, Zhang B, Boyington JC, Bylund T, Charaf S, Chen SJ, Du H, Henry AR, Liu T, Sarfo EK, Schramm CA, Shen CH, Stephens T, Teng IT, Todd JP, Tsybovsky Y, Verardi R, Wang D, Wang S, Wang Z, Zheng CY, Zhou T, Douek DC, Mascola JR, Ho DD, Ho M, Kwong PD
Nat Commun (2024) 15 pp. 285-285 [ DOI: doi:10.1038/s41467-023-44534-y Pubmed: 38177144 ]
- Glycoprotein g1 (22 kDa, Protein from Lassa virus Josiah)
- D5 nanobody (13 kDa, Protein from Camelus bactrianus)
- Glycoprotein g2 (22 kDa, Protein from Lassa virus Josiah)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Lassa gpc trimer in complex with fab 8.11g and nanobody d5 (Complex from Lassa virus Josiah)
- 8.11g light chain (12 kDa, Protein from Homo sapiens)
- 8.11g heavy chain (13 kDa, Protein from Homo sapiens)
Lassa GPC trimer in complex with Fab GP23
Gorman J, Cheung CS, Duan Z, Ou L, Wang M, Chen X, Cheng C, Biju A, Sun Y, Wang P, Yang Y, Zhang B, Boyington JC, Bylund T, Charaf S, Chen SJ, Du H, Henry AR, Liu T, Sarfo EK, Schramm CA, Shen CH, Stephens T, Teng IT, Todd JP, Tsybovsky Y, Verardi R, Wang D, Wang S, Wang Z, Zheng CY, Zhou T, Douek DC, Mascola JR, Ho DD, Ho M, Kwong PD
Nat Commun (2024) 15 pp. 285-285 [ DOI: doi:10.1038/s41467-023-44534-y Pubmed: 38177144 ]