Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8vcv
Q-score: 0.579
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
- Glycoprotein g2 (44 kDa, Protein from Lassa virus Josiah)
- Rabbit polyclonal fv heavy chain (10 kDa, Protein from Oryctolagus cuniculus)
- Lineage iv lassa virus glycoprotein (josiah) in complex with rabbit polyclonal antibody (gpc-c epitope) (Complex from Oryctolagus cuniculus)
- Rabbit polyclonal fv light chain (9 kDa, Protein from Oryctolagus cuniculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus Josiah)
Lineage IV Lassa virus glycoprotein (Josiah) in complex with monoclonal antibody 8.9F
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 9cj7
Q-score: 0.512
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Fv region of 8.9f light chain (25 kDa, Protein from Homo sapiens)
- Fv region of 8.9f heavy chain (31 kDa, Protein from Homo sapiens)
- Glycoprotein g2 (46 kDa, Protein from Lassa virus Josiah)
- Lineage iv lassa virus glycoprotein (josiah) in complex with monoclonal antibody 8.9f (Complex from Oryctolagus cuniculus)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus Josiah)
Resolution: 3.74 Å
EM Method: Single-particle
Fitted PDBs: 9cj8
Q-score: 0.423
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
- Glycoprotein g2 (44 kDa, Protein from Lassa virus Josiah)
- Lineage iv lassa virus glycoprotein (josiah) in complex with rabbit polyclonal antibody (ll-1 epitope) (Complex from Oryctolagus cuniculus)
- Rabbit polyclonal fv heavy chain (9 kDa, Protein from Oryctolagus cuniculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Rabbit polyclonal fv light chain (8 kDa, Protein from Oryctolagus cuniculus)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus Josiah)
Resolution: 3.45 Å
EM Method: Single-particle
Fitted PDBs: 9ck7
Q-score: 0.454
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
- Glycoprotein gp1 (29 kDa, Protein from Lassa virus Josiah)
- Rabbit polyclonal fv heavy chain (10 kDa, Protein from Oryctolagus cuniculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Rabbit polyclonal fv light chain (9 kDa, Protein from Oryctolagus cuniculus)
- Glycoprotein g2 (46 kDa, Protein from Lassa virus Josiah)
- Lineage iv lassa virus glycoprotein (josiah) in complex with rabbit polyclonal antibody (gpc-a-1 epitope) (Complex from Oryctolagus cuniculus)
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 9ck8
Q-score: 0.528
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
- Rabbit polyclonal fv heavy chain (10 kDa, Protein from Oryctolagus cuniculus)
- Glycoprotein gp1 (29 kDa, Protein from Lassa virus Josiah)
- Rabbit polyclonal fv light chain (9 kDa, Protein from Oryctolagus cuniculus)
- Lineage iv lassa virus glycoprotein (josiah) in complex with rabbit polyclonal antibody (gpc-a-2 epitope) (Complex from Oryctolagus cuniculus)
- Glycoprotein g2 (46 kDa, Protein from Lassa virus Josiah)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Lassa GPC (strain Josiah) bound to rabbit polyclonal base-targeting antibody Base-1
Brouwer PJM, Perrett HR, Nijhuis H, Lee W-H, Burger J, Lloyd NM, Himansu S, Beaumont T, Carfi A, Bukreyev A, Sanders RW, van Gils MJ, Ward AB
To Be Published
- Heterotrimeric lassa gpc bound to heterodimeric rabbit polyclonal base-targeting antibody base-1 (Complex)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus)
- Glycoprotein g2, 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase fusion protein (44 kDa, Protein from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8))
- Polyclonal antibody base-1 light chain (9 kDa, Protein from Oryctolagus cuniculus)
- Polyclonal antibody base-1 heavy chain, polyclonal antibody base-1 light chain (Complex from Oryctolagus cuniculus)
- Polyclonal antibody base-1 heavy chain (9 kDa, Protein from Oryctolagus cuniculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Glycoprotein g1, glycoprotein g2, 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase fusion protein (Complex from Lassa virus)
Lassa GPC (strain Josiah) bound to rabbit polyclonal fusion-peptide-targeting antibody FP-1
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8tye
Q-score: 0.421
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ DOI: doi:10.1016/j.celrep.2024.114708 Pubmed: 39243373 ]
- Glycoprotein g1, glycoprotein g2, 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase fusion protein (Complex from Lassa virus Josiah)
- Polyclonal antibody fp-1 heavy chain, polyclonal antibody fp-1 light chain (Complex from Oryctolagus cuniculus)
- Polyclonal fv heavy chain (9 kDa, Protein from Oryctolagus cuniculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Glycoprotein gp1 (29 kDa, Protein from Lassa virus (strain Mouse/Sierra Leone/Josiah/1976))
- Heterotrimeric lassa gpc bound to heterodimeric rabbit polyclonal fusion-peptide-targeting antibody fp-1 (Complex)
- Glycoprotein gp2 (44 kDa, Protein from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8))
- Polyclonal fv light chain (8 kDa, Protein from Oryctolagus cuniculus)
Lassa GPC Trimer in complex with Fab 8.11G and nanobody D5
Resolution: 4.7 Å
EM Method: Single-particle
Fitted PDBs: 8t5c
Q-score: 0.301
Gorman J, Cheung CS, Duan Z, Ou L, Wang M, Chen X, Cheng C, Biju A, Sun Y, Wang P, Yang Y, Zhang B, Boyington JC, Bylund T, Charaf S, Chen SJ, Du H, Henry AR, Liu T, Sarfo EK, Schramm CA, Shen CH, Stephens T, Teng IT, Todd JP, Tsybovsky Y, Verardi R, Wang D, Wang S, Wang Z, Zheng CY, Zhou T, Douek DC, Mascola JR, Ho DD, Ho M, Kwong PD
Nat Commun (2024) 15 pp. 285-285 [ DOI: doi:10.1038/s41467-023-44534-y Pubmed: 38177144 ]
- 8.11g light chain (12 kDa, Protein from Homo sapiens)
- Glycoprotein g2 (22 kDa, Protein from Lassa virus Josiah)
- Lassa gpc trimer in complex with fab 8.11g and nanobody d5 (Complex from Lassa virus Josiah)
- 8.11g heavy chain (13 kDa, Protein from Homo sapiens)
- Glycoprotein g1 (22 kDa, Protein from Lassa virus Josiah)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- D5 nanobody (13 kDa, Protein from Camelus bactrianus)
Structure of Lassa Virus glycoprotein (Josiah) bound to Fab 25.10C
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7tyv
Q-score: 0.447
Enriquez AS, Buck TK, Li H, Norris MJ, Moon-Walker A, Zandonatti MA, Harkins SS, Robinson JE, Branco LM, Garry RF, Saphire EO, Hastie KM
Cell Rep (2022) 39 pp. 110841-110841 [ Pubmed: 35613585 DOI: doi:10.1016/j.celrep.2022.110841 ]
- 25.10c fab heavy chain (24 kDa, Protein from Homo sapiens)
- Lassa virus josiah gp complexed with anti-lassa virus antibody fab fragment 25.10c (350 kDa, Complex)
- Glycoprotein g2 (19 kDa, Protein from Lassa virus)
- 25.10c fab light chain (25 kDa, Protein from Homo sapiens)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus)
- Lassa virus glycoprotein josiah (Complex from Lassa virus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 25.10c fab fragment (Complex from Homo sapiens)