S. aureus TarL H300N in complex with CDP-ribitol (single tetramer)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8va1
Q-score: 0.417
Li FKK, Worrall LJ, Gale RT, Brown ED, Strynadka NCJ
Sci Adv (2024) 10 pp. eadj3864-eadj3864 [ Pubmed: 38416829 DOI: doi:10.1126/sciadv.adj3864 ]
S. aureus TarL H300N in complex with CDP-ribitol (two tetramers with CHAPS micelle)
Li FKK, Worrall LJ, Gale RT, Brown ED, Strynadka NCJ
Sci Adv (2024) 10 pp. eadj3864-eadj3864 [ DOI: doi:10.1126/sciadv.adj3864 Pubmed: 38416829 ]
Cryo-EM structure of S. aureus BlaR1 with C2 symmetry
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 8exp
Q-score: 0.178
Alexander JAN, Worrall LJ, Hu J, Vuckovic M, Satishkumar N, Poon R, Sobhanifar S, Rosell FI, Jenkins J, Chiang D, Mosimann WA, Chambers HF, Paetzel M, Chatterjee SS, Strynadka NCJ
Nature (2023) 613 pp. 375-382 [ Pubmed: 36599987 DOI: doi:10.1038/s41586-022-05583-3 ]
- Dimeric complex of s. aureus blar1 (142 kDa, Complex from Staphylococcus aureus)
- Beta-lactam sensor/signal transducer blar1 (71 kDa, Protein from Staphylococcus aureus)
- Zinc ion (65 Da, Ligand)
Cryo-EM structure of S. aureus BlaR1 with C1 symmetry
Resolution: 4.9 Å
EM Method: Single-particle
Fitted PDBs: 8exq
Q-score: 0.122
Alexander JAN, Worrall LJ, Hu J, Vuckovic M, Satishkumar N, Poon R, Sobhanifar S, Rosell FI, Jenkins J, Chiang D, Mosimann WA, Chambers HF, Paetzel M, Chatterjee SS, Strynadka NCJ
Nature (2023) 613 pp. 375-382 [ DOI: doi:10.1038/s41586-022-05583-3 Pubmed: 36599987 ]
- Beta-lactam sensor/signal transducer blar1 (71 kDa, Protein from Staphylococcus aureus)
- Zinc ion (65 Da, Ligand)
- Dimeric complex of s. aureus blar1 (142 kDa, Complex from Staphylococcus aureus)
Cryo-EM structure of S. aureus BlaR1 TM and zinc metalloprotease domain
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8exr
Q-score: 0.324
Alexander JAN, Worrall LJ, Hu J, Vuckovic M, Satishkumar N, Poon R, Sobhanifar S, Rosell FI, Jenkins J, Chiang D, Mosimann WA, Chambers HF, Paetzel M, Chatterjee SS, Strynadka NCJ
Nature (2023) 613 pp. 375-382 [ DOI: doi:10.1038/s41586-022-05583-3 Pubmed: 36599987 ]
- (2s)-3-{[{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-2-[(6e)-hexadec-6-enoyloxy]propyl (8e)-octadec-8-enoate (746 Da, Ligand)
- Phosphate ion (94 Da, Ligand)
- Dimeric complex of s. aureus blar1 (142 kDa, Complex from Staphylococcus aureus)
- Beta-lactam sensor/signal transducer blar1 (71 kDa, Protein from Staphylococcus aureus)
- Zinc ion (65 Da, Ligand)
Cryo-EM structure of S. aureus BlaR1 F284A mutant
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 8exs
Q-score: 0.203
Alexander JAN, Worrall LJ, Hu J, Vuckovic M, Satishkumar N, Poon R, Sobhanifar S, Rosell FI, Jenkins J, Chiang D, Mosimann WA, Chambers HF, Paetzel M, Chatterjee SS, Strynadka NCJ
Nature (2023) 613 pp. 375-382 [ DOI: doi:10.1038/s41586-022-05583-3 Pubmed: 36599987 ]
- Dimeric complex of s. aureus blar1 f284a mutant (142 kDa, Complex from Staphylococcus aureus)
- Beta-lactam sensor/signal transducer blar1 (71 kDa, Protein from Staphylococcus aureus)
- Zinc ion (65 Da, Ligand)
Cryo-EM structure of S. aureus BlaR1 F284A mutant in complex with ampicillin
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 8ext
Q-score: 0.146
Alexander JAN, Worrall LJ, Hu J, Vuckovic M, Satishkumar N, Poon R, Sobhanifar S, Rosell FI, Jenkins J, Chiang D, Mosimann WA, Chambers HF, Paetzel M, Chatterjee SS, Strynadka NCJ
Nature (2023) 613 pp. 375-382 [ DOI: doi:10.1038/s41586-022-05583-3 Pubmed: 36599987 ]
- Dimeric complex of s. aureus blar1 f284a mutant in complex with ampicillin (142 kDa, Complex from Staphylococcus aureus)
- Beta-lactam sensor/signal transducer blar1 (71 kDa, Protein from Staphylococcus aureus)
- Zinc ion (65 Da, Ligand)
PmtCD ABC exporter without the basket domain at C2 symmetry
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6xjh
Q-score: 0.409
Zeytuni N, Dickey SW, Hu J, Chou HT, Worrall LJ, Alexander JAN, Carlson ML, Nosella M, Duong F, Yu Z, Otto M, Strynadka NCJ
Sci Adv (2020) 6 pp. eabb8219- [ DOI: doi:10.1126/sciadv.abb8219 Pubmed: 32998902 ]
- Abc transporter atp-binding protein (33 kDa, Protein from Staphylococcus aureus )
- Magnesium ion (24 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Pmtcd (120 kDa, Complex from Staphylococcus aureus)
- Phenol-soluble modulin export abc transporter permease subunit pmtd (30 kDa, Protein from Staphylococcus aureus)
PmtCD ABC exporter at C1 symmetry
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6xji
Q-score: 0.341
Zeytuni N, Dickey SW, Hu J, Chou HT, Worrall LJ, Alexander JAN, Carlson ML, Nosella M, Duong F, Yu Z, Otto M, Strynadka NCJ
Sci Adv (2020) 6 pp. eabb8219- [ DOI: doi:10.1126/sciadv.abb8219 Pubmed: 32998902 ]
- Magnesium ion (24 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Abc transporter atp-binding protein (33 kDa, Protein from Staphylococcus aureus)
- Pmtcd (120 kDa, Complex from Staphylococcus aureus)
- Phenol-soluble modulin export abc transporter permease subunit pmtd (30 kDa, Protein from Staphylococcus aureus)