Cryo-EM density map of Tn4430 TnpA hyperactive mutant (TnpA3X) in complex with IR48 substrate.
Fernandez M, Shkumatov AV, Liu Y, Stulemeijer C, Derclaye S, Efremov RG, Hallet B, Alsteens D
Nucleic Acids Res (2023) 51 pp. 4929-4941 [ Pubmed: 37026471 DOI: doi:10.1093/nar/gkad241 ]
- Tnpa3x-ir48 complex (Complex)
- Tnpa 3x mutant (Complex from Bacillus thuringiensis)
- Ir48 dna substrate, non transferred strand (DNA from Bacillus thuringiensis)
- Transposase for transposon tn4430 (Protein from Bacillus thuringiensis)
- Ir48 transferred strand (DNA from Bacillus thuringiensis)
- Ir48 substrate (Complex from Bacillus thuringiensis)
Medium resolution cryo-EM density map of Tn4430 TnpA transposase from Tn3 family in apo state
Fernandez M, Shkumatov AV, Liu Y, Stulemeijer C, Derclaye S, Efremov RG, Hallet B, Alsteens D
Nucleic Acids Res (2023) 51 pp. 4929-4941 [ Pubmed: 37026471 DOI: doi:10.1093/nar/gkad241 ]
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7qd4
Q-score: 0.446
Shkumatov AV, Aryanpour N, Oger CA, Goossens G, Hallet BF, Efremov RG
Nat Commun (2022) 13 pp. 6155-6155 [ DOI: doi:10.1038/s41467-022-33871-z Pubmed: 36257990 ]
- Transposase for transposon tn4430 (116 kDa, Protein from Bacillus thuringiensis)
- Ir100 dna substrate, none transferred strand (30 kDa, DNA from Bacillus thuringiensis)
- Ir100 dna substrate, transferred strand (30 kDa, DNA from Bacillus thuringiensis)
- Tnpa-ir100 complex (Complex)
- Hyperactive tnpa mutant s911r (Complex from Bacillus thuringiensis)
- Dna substrate (Complex from Bacillus thuringiensis)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7qd5
Q-score: 0.462
Shkumatov AV, Aryanpour N, Oger CA, Goossens G, Hallet BF, Efremov RG
Nat Commun (2022) 13 pp. 6155-6155 [ DOI: doi:10.1038/s41467-022-33871-z Pubmed: 36257990 ]
- Transposase for transposon tn4430 (116 kDa, Protein from Bacillus thuringiensis)
- Tnpa-ir48 complex (Complex)
- Ir48 transferred strand (14 kDa, DNA from Bacillus thuringiensis)
- Ir48 dna substrate, non transferred strand (14 kDa, DNA from Bacillus thuringiensis)
- Hyperactive tnpa mutant s911r (Complex from Bacillus thuringiensis)
- Dna substrate (Complex from Bacillus thuringiensis)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7qd6
Q-score: 0.351
Shkumatov AV, Aryanpour N, Oger CA, Goossens G, Hallet BF, Efremov RG
Nat Commun (2022) 13 pp. 6155-6155 [ DOI: doi:10.1038/s41467-022-33871-z Pubmed: 36257990 ]
- Transposase for transposon tn4430 (116 kDa, Protein from Bacillus thuringiensis)
- Ir71st transferred strand (24 kDa, DNA from Bacillus thuringiensis)
- Tnpa-ir71st complex (Complex)
- Ir71st non transferred strand (21 kDa, DNA from Bacillus thuringiensis)
- Hyperactive tnpa mutant s911r (Complex from Bacillus thuringiensis)
- Ir71st transferred strand (7 kDa, DNA from Bacillus thuringiensis)
- Dna substrate (Complex from Bacillus thuringiensis)
Cryo-EM structure of Tn4430 TnpA transposase from Tn3 family in apo state
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7qd8
Q-score: 0.352
Shkumatov AV, Aryanpour N, Oger CA, Goossens G, Hallet BF, Efremov RG
Nat Commun (2022) 13 pp. 6155-6155 [ DOI: doi:10.1038/s41467-022-33871-z Pubmed: 36257990 ]
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6yrf
Q-score: 0.355
Byrne MJ, Iadanza MG, Perez MA, Maskell DP, George RM, Hesketh EL, Beales PA, Zack MD, Berry C, Thompson RF
Nat Commun (2021) 12 pp. 2791-2791 [ DOI: doi:10.1038/s41467-021-23146-4 Pubmed: 33990582 ]
Vip3Bc1 tetramer in processed, activated state
Resolution: 4.8 Å
EM Method: Single-particle
Fitted PDBs: 6yrg
Q-score: 0.044
Byrne MJ, Iadanza MG, Perez MA, Maskell DP, George RM, Hesketh EL, Beales PA, Zack MD, Berry C, Thompson RF
Nat Commun (2021) 12 pp. 2791-2791 [ DOI: doi:10.1038/s41467-021-23146-4 Pubmed: 33990582 ]
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6tfj
Q-score: 0.434
Nunez-Ramirez R, Huesa J, Bel Y, Ferre J, Casino P, Arias-Palomo E
Nat Commun (2020) 11 pp. 3974-3974 [ Pubmed: 32769995 DOI: doi:10.1038/s41467-020-17758-5 ]
- Vegetative insecticidal protein (88 kDa, Protein from Bacillus thuringiensis)
- Vip3aa protoxin (350 kDa, Complex from Bacillus thuringiensis)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6tfk
Q-score: 0.422
Nunez-Ramirez R, Huesa J, Bel Y, Ferre J, Casino P, Arias-Palomo E
Nat Commun (2020) 11 pp. 3974-3974 [ Pubmed: 32769995 DOI: doi:10.1038/s41467-020-17758-5 ]
- Vip3aa toxin (350 kDa, Complex from Bacillus thuringiensis)
- Vegetative insecticidal protein (88 kDa, Protein from Bacillus thuringiensis)
- Magnesium ion (24 Da, Ligand)