Non-targeted transpososome from ShCAST
George JT, Acree C, Park JU, Kong M, Wiegand T, Pignot YL, Kellogg EH, Greene EC, Sternberg SH
Science (2023) 382 pp. eadj8543-eadj8543 [ DOI: doi:10.1126/science.adj8543 Pubmed: 37972161 ]
- Tnsb from shcast (Protein from [Scytonema hofmanni] UTEX 2349)
- Non-targeted transpososome from shcast (400 kDa, Complex from [Scytonema hofmanni] UTEX 2349)
- Tnsc from shcast (Protein from [Scytonema hofmanni] UTEX 2349)
- Tniq from shcast (Protein from [Scytonema hofmanni] UTEX 2349)
V-K CAST Transpososome from Scytonema hofmanni, minor configuration
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8ea4
Q-score: 0.302
Park JU, Tsai AW, Rizo AN, Truong VH, Wellner TX, Schargel RD, Kellogg EH
Nature (2023) 613 pp. 775-782 [ Pubmed: 36442503 DOI: doi:10.1038/s41586-022-05573-5 ]
- Non-target_r (21 kDa, DNA from synthetic construct)
- Cas12k (73 kDa, Protein from Scytonema hofmannii)
- Sg_rna (85 kDa, RNA from synthetic construct)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
- Tniq (19 kDa, Protein from Scytonema hofmannii)
- Re_r1 (15 kDa, DNA from synthetic construct)
- Re_f (23 kDa, DNA from synthetic construct)
- Sh_cast transpososome (Complex from [Scytonema hofmanni] UTEX 2349)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Le_r (15 kDa, DNA from synthetic construct)
- Target-le (43 kDa, DNA from synthetic construct)
- Tnsb (66 kDa, Protein from Scytonema hofmannii)
- Tnsc (31 kDa, Protein from Scytonema hofmannii)
- Magnesium ion (24 Da, Ligand)
- Re_r2 (6 kDa, DNA from synthetic construct)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7svu
Q-score: 0.379
Park J, Tsai AWT, Rizo AN, Truong VH, Wellner TX, Schargel RD, Kellogg EH
Nature (2023) 613 pp. 775-782 [ Pubmed: 36442503 DOI: doi:10.1038/s41586-022-05573-5 ]
- Tnsb-ctd (1 kDa, Protein from [Scytonema hofmanni] UTEX 2349)
- Atp-bound tnsbctd-tnsc-tniq complex from shcast element (400 kDa, Complex from [Scytonema hofmanni] UTEX 2349)
- Dna (29-mer) (9 kDa, DNA from synthetic construct)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna (28-mer) (8 kDa, DNA from synthetic construct)
- Tnsc (31 kDa, Protein from [Scytonema hofmanni] UTEX 2349)
- Tniq (19 kDa, Protein from [Scytonema hofmanni] UTEX 2349)
- Magnesium ion (24 Da, Ligand)
Resolution: 3.54 Å
EM Method: Helical reconstruction
Fitted PDBs: 7svv
Q-score: 0.454
Park JU, Tsai AW, Chen TH, Peters JE, Kellogg EH
PNAS (2022) 119 pp. e2202590119-e2202590119 [ DOI: doi:10.1073/pnas.2202590119 Pubmed: 35914146 ]
- Magnesium ion (24 Da, Ligand)
- Dna (5'-d(p*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*t)-3') (5 kDa, DNA from synthetic construct)
- Dna (5'-d(p*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*ap*a)-3') (6 kDa, DNA from synthetic construct)
- Tnsc filament (31 kDa, Protein from [Scytonema hofmanni] UTEX 2349)
- Tnsbctd (1 kDa, Protein from [Scytonema hofmanni] UTEX 2349)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Amppnp-bound tnsbctd-tnsc filament from shcast element (400 kDa, Complex from [Scytonema hofmanni] UTEX 2349)
Strand-transfer complex of TnsB from ShCAST
Resolution: 3.69 Å
EM Method: Single-particle
Fitted PDBs: 7svw
Q-score: 0.385
Park JU, Tsai AW, Chen TH, Peters JE, Kellogg EH
PNAS (2022) 119 pp. e2202590119-e2202590119 [ DOI: doi:10.1073/pnas.2202590119 Pubmed: 35914146 ]
- Tnsb (66 kDa, Protein from [Scytonema hofmanni] UTEX 2349)
- Stc_le_for (21 kDa, DNA from synthetic construct)
- Stc_le_rev2 (6 kDa, DNA from synthetic construct)
- Stc_le_rev1 (13 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Strand-transfer complex of tnsb from shcast element (350 kDa, Complex from [Scytonema hofmanni] UTEX 2349)
Park JU, Tsai AW, Chen TH, Peters JE, Kellogg EH
PNAS (2022) 119 pp. e2202590119-e2202590119 [ DOI: doi:10.1073/pnas.2202590119 Pubmed: 35914146 ]