La Crosse virus polymerase at replication late-elongation stage
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7ori
Q-score: 0.429
Arragain B, Durieux Trouilleton Q, Baudin F, Provaznik J, Azevedo N, Cusack S, Schoehn G, Malet H
Nat Commun (2022) 13 pp. 902-902 [ Pubmed: 35173159 DOI: doi:10.1038/s41467-022-28428-z ]
- La crosse virus polymerase (Complex from La Crosse orthobunyavirus)
- Zinc ion (65 Da, Ligand)
- Pyrophosphate 2- (175 Da, Ligand)
- La crosse virus polymerase (264 kDa, Protein from La Crosse orthobunyavirus)
- La crosse virus polymerase at late elongation stage (291 kDa, Complex)
- Magnesium ion (24 Da, Ligand)
- Rna (5'-r(p*ap*ap*cp*gp*up*up*ap*up*cp*up*ap*up*ap*ap*cp*ap*cp*up*ap*cp*u)-3') (9 kDa, RNA from La Crosse orthobunyavirus)
- Rna (5'-r(p*ap*up*ap*gp*ap*up*ap*ap*cp*gp*up*u)-3') (9 kDa, RNA from La Crosse orthobunyavirus)
- Rna (Complex from La Crosse orthobunyavirus)
- Rna (5'-r(p*ap*cp*gp*ap*gp*up*gp*up*cp*gp*u)-3') (5 kDa, RNA from La Crosse orthobunyavirus)
La Crosse virus polymerase at transcription capped RNA cleavage stage
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7orj
Q-score: 0.427
Arragain B, Durieux Trouilleton Q, Baudin F, Provaznik J, Azevedo N, Cusack S, Schoehn G, Malet H
Nat Commun (2022) 13 pp. 902-902 [ Pubmed: 35173159 DOI: doi:10.1038/s41467-022-28428-z ]
- Zinc ion (65 Da, Ligand)
- Rna (5'-r(p*cp*up*up*gp*gp*up*ap*gp*up*ap*cp*ap*cp*up*ap*cp*u)-3') (7 kDa, RNA from La Crosse orthobunyavirus)
- La crosse virus polymerase at transcription capped rna cleavage stage (284 kDa, Complex)
- 5' capped rna (4 kDa, RNA from La Crosse orthobunyavirus)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Rna (5'-r(p*ap*cp*gp*ap*gp*up*gp*up*cp*gp*up*ap*cp*cp*ap*ap*g)-3') (5 kDa, RNA from La Crosse orthobunyavirus)
- Rna-directed rna polymerase l (264 kDa, Protein from Bunyavirus La Crosse)
La Crosse virus polymerase at replication initiation stage
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7orn
Q-score: 0.622
Arragain B, Durieux Trouilleton Q, Baudin F, Provaznik J, Azevedo N, Cusack S, Schoehn G, Malet H
Nat Commun (2022) 13 pp. 902-902 [ Pubmed: 35173159 DOI: doi:10.1038/s41467-022-28428-z ]
- La crosse virus polymerase at replication initiation (279 kDa, Complex)
- Rna (5'-r(p*ap*cp*up*up*gp*gp*up*ap*gp*up*ap*cp*ap*cp*up*ap*cp*u)-3') (7 kDa, RNA from La Crosse orthobunyavirus)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- La crosse virus polymerase (264 kDa, Protein from La Crosse orthobunyavirus)
- Rna (Complex from La Crosse orthobunyavirus)
- Rna (5'-r(p*ap*cp*gp*ap*gp*up*gp*up*cp*gp*up*ap*cp*cp*ap*ap*g)-3') (5 kDa, RNA from La Crosse orthobunyavirus)
- Water (18 Da, Ligand)
- La crosse virus polymerase (Complex from La Crosse orthobunyavirus)
La Crosse virus polymerase at replication early-elongation stage
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7oro
Q-score: 0.548
Arragain B, Durieux Trouilleton Q, Baudin F, Provaznik J, Azevedo N, Cusack S, Schoehn G, Malet H
Nat Commun (2022) 13 pp. 902-902 [ Pubmed: 35173159 DOI: doi:10.1038/s41467-022-28428-z ]
- La crosse virus polymerase at replication early-elongation stage (293 kDa, Complex)
- Zinc ion (65 Da, Ligand)
- Rna (5'-r(p*ap*gp*up*ap*gp*up*gp*up*a)-3') (2 kDa, RNA from La Crosse orthobunyavirus)
- Rna (5'-r(p*gp*gp*up*ap*gp*up*ap*cp*ap*cp*up*ap*cp*u)-3') (7 kDa, RNA from La Crosse orthobunyavirus)
- Rna (5'-r(p*ap*cp*gp*ap*gp*up*gp*up*cp*gp*up*ap*cp*c)-3') (5 kDa, RNA from La Crosse orthobunyavirus)
- Magnesium ion (24 Da, Ligand)
- La crosse virus polymerase (264 kDa, Protein from La Crosse orthobunyavirus)
- Rna (Complex from La Crosse orthobunyavirus)
- La crosse virus polymerase (Complex from La Crosse orthobunyavirus)
Cryo-EM map of La Crosse virus polymerase at pre-initiation stage
Resolution: 3.06 Å
EM Method: Single-particle
Fitted PDBs: 6z6g
Q-score: 0.499
Arragain B, Effantin G, Gerlach P, Reguera J, Schoehn G, Cusack S, Malet H
Nat Commun (2020) 11 pp. 3590-3590 [ DOI: doi:10.1038/s41467-020-17349-4 Pubmed: 32681014 ]
- 5'vrna 9-16 (2 kDa, RNA from La Crosse orthobunyavirus)
- La crosse virus polymerase at pre-initiation stage (265 kDa, Complex)
- La crosse virus polymerase at pre-initiation stage (Complex from Bunyavirus La Crosse)
- Zinc ion (65 Da, Ligand)
- 3'vrna 1-16 (5 kDa, RNA from La Crosse orthobunyavirus)
- La crosse virus polymerase at pre-initiation stage (Complex from La Crosse orthobunyavirus)
- Magnesium ion (24 Da, Ligand)
- 5'vrna 1-10 (3 kDa, RNA from La Crosse orthobunyavirus)
- Rna-directed rna polymerase l (266 kDa, Protein from Bunyavirus La Crosse)
Masked cryo-EM map of La Crosse virus polymerase cap-binding domain and mid domain
Arragain B, Effantin G, Gerlach P, Reguera J, Schoehn G, Cusack S, Malet H
Nat Commun (2020) 11 pp. 3590-3590 [ DOI: doi:10.1038/s41467-020-17349-4 Pubmed: 32681014 ]
Masked cryo-EM map of La Crosse virus polymerase zinc-binding domain and mid domain
Arragain B, Effantin G, Gerlach P, Reguera J, Schoehn G, Cusack S, Malet H
Nat Commun (2020) 11 pp. 3590-3590 [ DOI: doi:10.1038/s41467-020-17349-4 Pubmed: 32681014 ]
La Crosse virus polymerase at elongation mimicking stage
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 6z8k
Q-score: 0.494
Arragain B, Effantin G, Gerlach P, Reguera J, Schoehn G, Cusack S, Malet H
Nat Commun (2020) 11 pp. 3590-3590 [ DOI: doi:10.1038/s41467-020-17349-4 Pubmed: 32681014 ]
- Zinc ion (65 Da, Ligand)
- La crosse virus polymerase at elongation stage (Complex from La Crosse orthobunyavirus)
- Rna-directed rna polymerase l (Complex from La Crosse orthobunyavirus)
- La crosse virus polymerase at elongation-mimicking stage (265 kDa, Complex)
- La crosse virus 5' vrna 1-10 (3 kDa, RNA from La Crosse orthobunyavirus)
- Rna-directed rna polymerase l (266 kDa, Protein from La Crosse orthobunyavirus)
- La crosse virus 5' vrna (9-16) (2 kDa, RNA from La Crosse orthobunyavirus)
- La crosse virus 3' vrna (1-16) (5 kDa, RNA from La Crosse orthobunyavirus)
- Magnesium ion (24 Da, Ligand)