Cryo-EM Structure of Chikungunya Virus Nonstructural Protein 1 with m7GpppAmU
Resolution: 2.41 Å
EM Method: Single-particle
Fitted PDBs: 8jce
Q-score: 0.643
Law MCY, Zhang K, Tan YB, Nguyen TM, Luo D
J Biol Chem (2023) 299 pp. 105415-105415 [ DOI: doi:10.1016/j.jbc.2023.105415 Pubmed: 37918803 ]
- Zinc ion (65 Da, Ligand)
- Mrna-capping enzyme nsp1 (61 kDa, Protein from Chikungunya virus strain S27-African prototype)
- [(2~{r},3~{r},4~{r},5~{r})-5-(6-azanyl-7,8-dihydropurin-9-yl)-2-[[[[[(2~{r},3~{s},4~{r},5~{r})-5-(2-azanyl-7-methyl-6-oxidanylidene-1,8-dihydropurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-4-methoxy-oxolan-3-yl] [(2~{r},3~{s},4~{r},5~{s})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate (1 kDa, Ligand)
- Nonstructural protein 1 (Complex from Chikungunya virus strain S27-African prototype)
- S-adenosyl-l-homocysteine (384 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
Molecular architecture of the chikungunya virus replication complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7y38
Q-score: 0.539
Tan YB, Chmielewski D, Law MCY, Zhang K, He Y, Chen M, Jin J, Luo D
Sci Adv (2022) 8 pp. eadd2536-eadd2536 [ DOI: doi:10.1126/sciadv.add2536 Pubmed: 36449616 ]
- Zinc ion (65 Da, Ligand)
- Nsp1 dodecameric ring (Complex from Chikungunya virus strain S27-African prototype)
- Rna (5'-r(p*cp*cp*a)-3') (894 Da, RNA from in vitro transcription vector pT7-TP(deltai))
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Mrna-capping enzyme nsp1,affinity-tag (strepii-3xflag) (64 kDa, Protein from Chikungunya virus strain S27-African prototype)
- Rna-directed rna polymerase nsp4 (68 kDa, Protein from Onyong-nyong virus)
- Protease nsp2 (89 kDa, Protein from Chikungunya virus strain S27-African prototype)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Nsp4 rdrp; docked within nsp1 central cavity (Complex from Onyong-nyong virus)
- Alphavirus replication complex (100 kDa, Complex)
- Nsp2 helicase-protease; docked on top of viral complex with synthetic rna bound (Complex from Chikungunya virus strain S27-African prototype)
Cryo-EM Structure of Chikungunya Virus Nonstructural Protein 1 with inhibitor FHA
Resolution: 2.62 Å
EM Method: Single-particle
Fitted PDBs: 7x01
Q-score: 0.599
Zhang K, Law MCY, Nguyen TM, Tan YB, Wirawan M, Law YS, Jeong LS, Luo D
Cell Rep (2022) 40 pp. 111133-111133 [ DOI: doi:10.1016/j.celrep.2022.111133 Pubmed: 35905713 ]
- Nonstructural protein 1 (Complex from Chikungunya virus strain S27-African prototype)
- Zinc ion (65 Da, Ligand)
- Mrna-capping enzyme nsp1 (62 kDa, Protein from Chikungunya virus strain S27-African prototype)
- (1r,2s,3s,4r,5r)-3-(6-aminopurin-9-yl)-4-fluoranyl-5-(2-hydroxyethyl)cyclopentane-1,2-diol (297 Da, Ligand)
Cryo-EM Structure of Chikungunya Virus Nonstructural Protein 1 with m7GTP
Resolution: 2.19 Å
EM Method: Single-particle
Fitted PDBs: 7fgg
Q-score: 0.698
Zhang K, Law MCY, Nguyen TM, Tan YB, Wirawan M, Law YS, Jeong LS, Luo D
Cell Rep (2022) 40 pp. 111133-111133 [ DOI: doi:10.1016/j.celrep.2022.111133 Pubmed: 35905713 ]
- Zinc ion (65 Da, Ligand)
- Nonstructural protein 1 (Complex from Chikungunya virus strain S27-African prototype)
- S-adenosyl-l-homocysteine (384 Da, Ligand)
- Mrna-capping enzyme nsp1 (62 kDa, Protein from Chikungunya virus strain S27-African prototype)
- 7-methyl-guanosine-5'-triphosphate (538 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
Cryo-EM Structure of Chikungunya Virus Nonstructural Protein 1 with m7GMP
Resolution: 2.18 Å
EM Method: Single-particle
Fitted PDBs: 7fgh
Q-score: 0.688
Zhang K, Law MCY, Nguyen TM, Tan YB, Wirawan M, Law YS, Jeong LS, Luo D
Cell Rep (2022) 40 pp. 111133-111133 [ DOI: doi:10.1016/j.celrep.2022.111133 Pubmed: 35905713 ]
- Zinc ion (65 Da, Ligand)
- N7-methyl-guanosine-5'-monophosphate (378 Da, Ligand)
- Nonstructural protein 1 (Complex from Chikungunya virus strain S27-African prototype)
- S-adenosyl-l-homocysteine (384 Da, Ligand)
- Mrna-capping enzyme nsp1 (62 kDa, Protein from Chikungunya virus strain S27-African prototype)
- Water (18 Da, Ligand)
Cryo-EM Structure of Chikungunya Virus Nonstructural Protein 1 with m7Gppp-AU
Resolution: 2.51 Å
EM Method: Single-particle
Fitted PDBs: 7fgi
Q-score: 0.627
Zhang K, Law MCY, Nguyen TM, Tan YB, Wirawan M, Law YS, Jeong LS, Luo D
Cell Rep (2022) 40 pp. 111133-111133 [ DOI: doi:10.1016/j.celrep.2022.111133 Pubmed: 35905713 ]
- Zinc ion (65 Da, Ligand)
- Mrna-capping enzyme nsp1 (61 kDa, Protein from Chikungunya virus strain S27-African prototype)
- Nonstructural protein 1 (Complex from Chikungunya virus strain S27-African prototype)
- M7gppp-au (1 kDa, RNA from synthetic construct)
- S-adenosyl-l-homocysteine (384 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
Resolution: 2.38 Å
EM Method: Single-particle
Fitted PDBs: 7dop
Q-score: 0.615