Structure of the formin Cdc12 bound to the barbed end of phalloidin-stabilized F-actin.
Resolution: 3.56 Å
EM Method: Single-particle
Fitted PDBs: 8rtt
Q-score: 0.444
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Magnesium ion (24 Da, Ligand)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Cell division control protein 12 (48 kDa, Protein from Schizosaccharomyces pombe)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphate ion (94 Da, Ligand)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Phalloidin. (Complex from Amanita phalloides)
- Complex of the dimeric fh2 domain of s. pombe cdc12 bound to the barbed end of phalloidin stabilized f-actin. (Complex)
- Dimeric fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
- Actin filament (Complex from Homo sapiens)
Resolution: 6.25 Å
EM Method: Single-particle
Fitted PDBs: 8rty
Q-score: 0.17
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ Pubmed: 38603491 DOI: doi:10.1126/science.adn9560 ]
- Dimeric fh1fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
- Magnesium ion (24 Da, Ligand)
- Phalloidin (amanita phalloides) (808 Da, Protein from Amanita phalloides)
- Phalloidin (Complex from Amanita phalloides)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin-formin-profilin ring complex: the phalloidin-stabilized f-actin barbed end bound by dimeric-cdc12 and profilin-s71m. (Complex)
- Phosphate ion (94 Da, Ligand)
- Methylated-dna--protein-cysteine methyltransferase,cell division control protein 12 (77 kDa, Protein from Schizosaccharomyces pombe)
- Profilin-1 (15 kDa, Protein from Homo sapiens)
- Profilin-1-s29c/s71m (Complex from Homo sapiens)
- Actin filament (Complex from Homo sapiens)
Straight actin filament from Arp2/3 branch junction sample (ADP-BeFx)
Resolution: 3.6 Å
EM Method: Helical reconstruction
Fitted PDBs: 8uz1
Q-score: 0.489
Chavali SS, Chou SZ, Cao W, Pollard TD, De La Cruz EM, Sindelar CV
Nat Commun (2024) 15 pp. 2059-2059 [ DOI: doi:10.1038/s41467-024-46179-x Pubmed: 38448439 ]
- Actin (Cellular component from Gallus gallus)
- Beryllium trifluoride ion (66 Da, Ligand)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Arp2/3 branch complex (adp-befx state) (Complex)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Arp2/3 complex (Complex from Schizosaccharomyces pombe)
Cryo-EM structure of the Clr6S (Clr6-HDAC) complex from S. pombe
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8ifg
Q-score: 0.441
Wang X, Wang Y, Liu S, Zhang Y, Xu K, Ji L, Kornberg RD, Zhang H
PNAS (2023) 120 pp. e2307598120- [ Pubmed: 37459529 DOI: doi:10.1073/pnas.2307598120 ]
- Histone deacetylase clr6 (45 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Rbap48-related wd40 repeat-containing protein prw1 (48 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Cph2 (68 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Chromatin modification-related protein eaf3 (39 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Water (18 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Cph1 (45 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Cryo-em structure of the clr6-hdac (clr6s) complex from s. pombe (600 kDa, Complex from Schizosaccharomyces pombe)
- Paired amphipathic helix protein pst2 (125 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
Cryo-EM structure of the SIN3S complex from S. pombe
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8i02
Q-score: 0.496
Wang C, Guo Z, Chu C, Lu Y, Zhang X, Zhan X
Cell Discov (2023) 9 pp. 42-42 [ Pubmed: 37076472 DOI: doi:10.1038/s41421-023-00539-x ]
- Paired amphipathic helix protein pst2 (125 kDa, Protein from Schizosaccharomyces pombe)
- Uncharacterized protein c2f7.07c (68 kDa, Protein from Schizosaccharomyces pombe)
- Rbap48-related wd40 repeat-containing protein prw1 (48 kDa, Protein from Schizosaccharomyces pombe)
- The sin3s complex (Complex from Schizosaccharomyces pombe)
- Histone deacetylase clr6 (46 kDa, Protein from Schizosaccharomyces pombe)
- Potassium ion (39 Da, Ligand)
- Cph1 (45 kDa, Protein from Schizosaccharomyces pombe)
- Zinc ion (65 Da, Ligand)
- Chromatin modification-related protein eaf3 (39 kDa, Protein from Schizosaccharomyces pombe)
Cytochrome c oxidase from Schizosaccharomyces pombe
Resolution: 3.36 Å
EM Method: Single-particle
Fitted PDBs: 8c8q
Q-score: 0.525
Moe A, Adelroth P, Brzezinski P, Nasvik Ojemyr L
Commun Chem (2023) 6 pp. 32-32 [ Pubmed: 36797353 DOI: doi:10.1038/s42004-023-00827-3 ]
- Copper (ii) ion (63 Da, Ligand)
- Cytochrome c oxidase polypeptide viii, mitochondrial (7 kDa, Protein from Schizosaccharomyces pombe)
- Zinc ion (65 Da, Ligand)
- Cytochrome c oxidase subunit 1 (59 kDa, Protein from Schizosaccharomyces pombe)
- Cytochrome c oxidase subunit 2 (28 kDa, Protein from Schizosaccharomyces pombe)
- Cytochrome c oxidase subunit 6, mitochondrial (15 kDa, Protein from Schizosaccharomyces pombe)
- Magnesium ion (24 Da, Ligand)
- Heme-a (852 Da, Ligand)
- Cytochrome c oxidase subunit 9, mitochondrial (6 kDa, Protein from Schizosaccharomyces pombe)
- Cytochrome c oxidase subunit 13, mitochondrial (15 kDa, Protein from Schizosaccharomyces pombe)
- Cytochrome c oxidase polypeptide 5, mitochondrial (24 kDa, Protein from Schizosaccharomyces pombe)
- Di-palmitoyl-3-sn-phosphatidylethanolamine (691 Da, Ligand)
- Cytochrome c oxidase subunit 4, mitochondrial (17 kDa, Protein from Schizosaccharomyces pombe)
- Unknown polypeptide (2 kDa, Protein from Schizosaccharomyces pombe)
- Cytochrome c oxidase (246 kDa, Complex from Schizosaccharomyces pombe)
- Cytochrome c oxidase subunit 7 (6 kDa, Protein from Schizosaccharomyces pombe)
- Cytochrome c oxidase subunit 3 (30 kDa, Protein from Schizosaccharomyces pombe)
- Respiratory supercomplex factor 2 homolog c1565.01 (27 kDa, Protein from Schizosaccharomyces pombe)
- Calcium ion (40 Da, Ligand)
- Dinuclear copper ion (127 Da, Ligand)
- Cytochrome c oxidase subunit 12, mitochondrial (10 kDa, Protein from Schizosaccharomyces pombe)
S. pombe microtubule copolymerized with GTP and Mal3-143
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 5mjs
Q-score: 0.331
von Loeffelholz O, Venables NA, Drummond DR, Katsuki M, Cross R, Moores CA
Nat Commun (2017) 8 pp. 2110-2110 [ DOI: doi:10.1038/s41467-017-02241-5 Pubmed: 29235477 ]
- Guanosine-5'-diphosphate (443 Da, Ligand from Schizosaccharomyces pombe)
- Microtubule decorated with mal3 (Complex from Schizosaccharomyces pombe)
- Tubulin beta chain (47 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Guanosine-5'-triphosphate (523 Da, Ligand from Schizosaccharomyces pombe)
- Microtubule integrity protein mal3 (16 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Tubulin alpha-1 chain (49 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
Cryo-EM map of the transcriptional Mediator
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 5u0p
Q-score: 0.236
Tsai KL, Yu X, Gopalan S, Chao TC, Zhang Y, Florens L, Washburn MP, Murakami K, Conaway RC, Conaway JW, Asturias FJ
Nature (2017) 544 pp. 196-201 [ Pubmed: 28241144 DOI: doi:10.1038/nature21393 ]
- Mediator complex subunit 14 (105 kDa, Protein from Schizosaccharomyces pombe)
- Mediator complex subunit 17 (62 kDa, Protein from Schizosaccharomyces pombe)
- Mediator complex subunit 7 (43 kDa, Protein from Schizosaccharomyces pombe)
- Mediator complex (Complex from Schizosaccharomyces pombe)
- Mediator complex subunit 22 (15 kDa, Protein from Schizosaccharomyces pombe)
- Mediator complex subunit 31 (16 kDa, Protein from Schizosaccharomyces pombe)
- Mediator complex subunit 8 (23 kDa, Protein from Schizosaccharomyces pombe)
- Mediator complex subunit 6 (24 kDa, Protein from Schizosaccharomyces pombe)
- Mediator complex subunit 11 (13 kDa, Protein from Schizosaccharomyces pombe)
- Mediator complex subunit 9 (13 kDa, Protein from Schizosaccharomyces pombe)
- Mediator complex subunit 4 (20 kDa, Protein from Schizosaccharomyces pombe)
- Mediator complex subunit 27 (31 kDa, Protein from Schizosaccharomyces pombe)
- Mediator complex subunit 19 (11 kDa, Protein from Schizosaccharomyces pombe)
- Mediator complex subunit 10 (11 kDa, Protein from Schizosaccharomyces pombe)
- Mediator complex subunit 21 (15 kDa, Protein from Schizosaccharomyces pombe)
- Mediator complex subunit 20 (22 kDa, Protein from Schizosaccharomyces pombe)
- Mediator complex subunit 18 (24 kDa, Protein from Schizosaccharomyces pombe)
Subnanometer reconstruction of GTPgammaS microtubules decorated with monomeric Mal3
Resolution: 8.6 Å
EM Method: Single-particle
Fitted PDBs: 4abo
Q-score: 0.119
Maurer SP, Fourniol FJ, Bohner G, Moores CA, Surrey T
Cell (2012) 149 pp. 371-382 [ Pubmed: 22500803 DOI: doi:10.1016/j.cell.2012.02.049 ]
- Alpha tubulin (50 kDa, Protein from Sus scrofa)
- Gtpgammas microtubules decorated with monomeric mal3 (Sample)
- Guanosine 5'-o-(gamma-thio)triphosphate (Ligand from synthetic construct)
- Beta tubulin (50 kDa, Protein from Sus scrofa)
- Mal3 (16 kDa, Protein from Schizosaccharomyces pombe)