Cryo EM structure of the vaccinia complete RNA polymerase complex lacking the capping enzyme
Resolution: 3.84 Å
EM Method: Single-particle
Fitted PDBs: 8c8h
Q-score: 0.333
To Be Published
- Vaccinia complete rna polymerase complex lacking the capping enzyme (700 kDa, Complex from Vaccinia virus GLV-1h68)
- Magnesium ion (24 Da, Ligand)
- Chr17.trna16-glnttg (23 kDa, RNA from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 19 kda subunit (19 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 35 kda subunit (35 kDa, Protein from Vaccinia virus GLV-1h68)
- Water (18 Da, Ligand)
- Core protein e11 (14 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 18 kda subunit (17 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 22 kda subunit (21 kDa, Protein from Vaccinia virus GLV-1h68)
- Rna polymerase-associated transcription-specificity factor rap94 (93 kDa, Protein from Vaccinia virus GLV-1h68)
- Early transcription factor 82 kda subunit (82 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 30 kda polypeptide (30 kDa, Protein from Vaccinia virus GLV-1h68)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase 7 kda subunit (7 kDa, Protein from Vaccinia virus GLV-1h68)
- Nucleoside triphosphatase i (72 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 147 kda polypeptide (146 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 133 kda polypeptide (133 kDa, Protein from Vaccinia virus GLV-1h68)
Atomic structure of the poxvirus transcription pre-initiation complex in the initially melted state
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7amv
Q-score: 0.537
Grimm C, Bartuli J, Boettcher B, Szalay AA, Fischer U
Nat Struct Mol Biol (2021) 28 pp. 779-788 [ Pubmed: 34556871 DOI: doi:10.1038/s41594-021-00655-w ]
- Synthetic promoter dna oligomer, template strand (18 kDa, DNA from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 30 kda polypeptide (29 kDa, Protein from Vaccinia virus GLV-1h68)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase 19 kda subunit (19 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 35 kda subunit (35 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 18 kda subunit (17 kDa, Protein from Vaccinia virus GLV-1h68)
- Vaccinia transcription pre-initiation complex (670 kDa, Complex from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase (133 kDa, Protein from Vaccinia virus GLV-1h68)
- Synthetic promoter dna oligomer, non-template strand (18 kDa, DNA from Vaccinia virus GLV-1h68)
- Protein h4 (93 kDa, Protein from Vaccinia virus GLV-1h68)
- Etf large subunit (82 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 7 kda subunit (7 kDa, Protein from Vaccinia virus GLV-1h68)
- Atp-dependent helicase vetfs (73 kDa, Protein from Vaccinia virus GLV-1h68)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit (21 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 147 kda polypeptide (146 kDa, Protein from Vaccinia virus GLV-1h68)
Atomic structure of the poxvirus transcription late pre-initiation complex
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 7aof
Q-score: 0.505
Grimm C, Bartuli J, Boettcher B, Szalay AA, Fischer U
Nat Struct Mol Biol (2021) 28 pp. 779-788 [ Pubmed: 34556871 DOI: doi:10.1038/s41594-021-00655-w ]
- Protein h4 (Complex from Vaccinia virus GLV-1h68)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase 19 kda subunit (19 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 18 kda subunit (17 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase (133 kDa, Protein from Vaccinia virus GLV-1h68)
- Vaccinia transcription pre-initiation complex (Complex from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 30 kda polypeptide (30 kDa, Protein from Vaccinia virus GLV-1h68)
- Protein h4 (93 kDa, Protein from Vaccinia virus GLV-1h68)
- Vaccinia transcription pre-initiation complex (670 kDa, Complex)
- Dna-directed rna polymerase 7 kda subunit (7 kDa, Protein from Vaccinia virus GLV-1h68)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase 35 kda subunit (35 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 147 kda polypeptide (146 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase subunit (21 kDa, Protein from Vaccinia virus GLV-1h68)
Atomic structure of the poxvirus late initially transcribing complex
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7aoh
Q-score: 0.543
Grimm C, Bartuli J, Boettcher B, Szalay AA, Fischer U
Nat Struct Mol Biol (2021) 28 pp. 779-788 [ Pubmed: 34556871 DOI: doi:10.1038/s41594-021-00655-w ]
- Dna (Complex from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 19 kda subunit (19 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase (133 kDa, Protein from Vaccinia virus GLV-1h68)
- Rna (Complex from Vaccinia virus GLV-1h68)
- Zinc ion (65 Da, Ligand)
- Nucleoside triphosphatase i (72 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 35 kda subunit (35 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 147 kda polypeptide (146 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase subunit (21 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 18 kda subunit (17 kDa, Protein from Vaccinia virus GLV-1h68)
- Synthetic promoter dna oligomer, non-template strand (18 kDa, DNA from Vaccinia virus GLV-1h68)
- Protein h4 (93 kDa, Protein from Vaccinia virus GLV-1h68)
- Vaccinia transcription pre-initiation complex (670 kDa, Complex)
- Dna-directed rna polymerase 7 kda subunit (7 kDa, Protein from Vaccinia virus GLV-1h68)
- Synthetic promoter dna oligomer, template strand (18 kDa, DNA from Vaccinia virus GLV-1h68)
- Magnesium ion (24 Da, Ligand)
- Rna (5'-r(p*ap*ap*ap*ap*up*cp*up*ap*up*a)-3') (3 kDa, RNA from Vaccinia virus GLV-1h68)
- Vaccinia transcription pre-initiation complex (Complex from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 30 kda polypeptide (29 kDa, Protein from Vaccinia virus GLV-1h68)
Atomic structure of the poxvirus transcription initiation complex in conformation 1
Resolution: 2.85 Å
EM Method: Single-particle
Fitted PDBs: 7aoz
Q-score: 0.545
Grimm C, Bartuli J, Boettcher B, Szalay AA, Fischer U
Nat Struct Mol Biol (2021) 28 pp. 779-788 [ Pubmed: 34556871 DOI: doi:10.1038/s41594-021-00655-w ]
- Synthetic promoter dna oligomer, template strand (18 kDa, DNA from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 30 kda polypeptide (29 kDa, Protein from Vaccinia virus GLV-1h68)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 19 kda subunit (19 kDa, Protein from Vaccinia virus GLV-1h68)
- Rna (5'-r(p*ap*up*ap*ap*a)-3') (1 kDa, RNA from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 18 kda subunit (17 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase (133 kDa, Protein from Vaccinia virus GLV-1h68)
- Vaccinia transcription pre-initiation complex (Complex from Vaccinia virus GLV-1h68)
- Synthetic promoter dna oligomer, non-template strand (18 kDa, DNA from Vaccinia virus GLV-1h68)
- Protein h4 (93 kDa, Protein from Vaccinia virus GLV-1h68)
- Vaccinia transcription pre-initiation complex (670 kDa, Complex)
- Dna-directed rna polymerase 7 kda subunit (7 kDa, Protein from Vaccinia virus GLV-1h68)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase 35 kda subunit (35 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 147 kda polypeptide (146 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase subunit (21 kDa, Protein from Vaccinia virus GLV-1h68)
Atomic structure of the poxvirus initially transcribing complex in conformation 2
Resolution: 3.15 Å
EM Method: Single-particle
Fitted PDBs: 7ap8
Q-score: 0.482
Grimm C, Bartuli J, Boettcher B, Szalay AA, Fischer U
Nat Struct Mol Biol (2021) 28 pp. 779-788 [ Pubmed: 34556871 DOI: doi:10.1038/s41594-021-00655-w ]
- Synthetic promoter dna oligomer, template strand (18 kDa, DNA from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 30 kda polypeptide (29 kDa, Protein from Vaccinia virus GLV-1h68)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 19 kda subunit (19 kDa, Protein from Vaccinia virus GLV-1h68)
- Rna (5'-r(p*ap*up*ap*ap*a)-3') (1 kDa, RNA from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 18 kda subunit (17 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase (133 kDa, Protein from Vaccinia virus GLV-1h68)
- Vaccinia transcription pre-initiation complex (Complex from Vaccinia virus GLV-1h68)
- Synthetic promoter dna oligomer, non-template strand (18 kDa, DNA from Vaccinia virus GLV-1h68)
- Rna (Complex from Vaccinia virus GLV-1h68)
- Protein h4 (93 kDa, Protein from Vaccinia virus GLV-1h68)
- Vaccinia transcription pre-initiation complex (670 kDa, Complex)
- Dna-directed rna polymerase 7 kda subunit (7 kDa, Protein from Vaccinia virus GLV-1h68)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase 35 kda subunit (35 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 147 kda polypeptide (146 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase subunit (21 kDa, Protein from Vaccinia virus GLV-1h68)
Atomic structure of the poxvirus initially transcribing complex in conformation 3
Resolution: 3.01 Å
EM Method: Single-particle
Fitted PDBs: 7ap9
Q-score: 0.515
Grimm C, Bartuli J, Boettcher B, Szalay AA, Fischer U
Nat Struct Mol Biol (2021) 28 pp. 779-788 [ Pubmed: 34556871 DOI: doi:10.1038/s41594-021-00655-w ]
- Dna-directed rna polymerase 30 kda polypeptide (29 kDa, Protein from Vaccinia virus GLV-1h68)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase 147 kda polypeptide (146 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase and protein h4 (Complex from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 19 kda subunit (19 kDa, Protein from Vaccinia virus GLV-1h68)
- Rna (5'-r(p*ap*up*ap*ap*a)-3') (1 kDa, RNA from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 18 kda subunit (17 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase (133 kDa, Protein from Vaccinia virus GLV-1h68)
- Synthetic promoter dna oligomer, template strand (18 kDa, DNA from synthetic construct)
- Rna (Complex from Vaccinia virus GLV-1h68)
- Protein h4 (93 kDa, Protein from Vaccinia virus GLV-1h68)
- Vaccinia transcription pre-initiation complex (670 kDa, Complex)
- Dna-directed rna polymerase 7 kda subunit (7 kDa, Protein from Vaccinia virus GLV-1h68)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase 35 kda subunit (35 kDa, Protein from Vaccinia virus GLV-1h68)
- Synthetic promoter dna oligomer, template strand (Complex from synthetic construct)
- Dna-directed rna polymerase subunit (21 kDa, Protein from Vaccinia virus GLV-1h68)
Structure of the core Vaccinia Virus DNA-dependent RNA polymerase complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 6ric
Q-score: 0.562
Hillen HS, Bartuli J, Grimm C, Dienemann C, Bedenk K, Szalay AA, Fischer U, Cramer P
Cell (2019) 179 pp. 1525- [ DOI: doi:10.1016/j.cell.2019.11.023 Pubmed: 31835031 ]
- Dna-dependent rna polymerase subunit rpo147 (146 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-dependent rna polymerase subunit rpo18 (17 kDa, Protein from Vaccinia virus GLV-1h68)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase 19 kda subunit (19 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-dependent rna polymerase subunit rpo132 (133 kDa, Protein from Vaccinia virus GLV-1h68)
- Rap94 transcription factor (93 kDa, Protein from Vaccinia virus GLV-1h68)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase 30 kda polypeptide (29 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-dependent rna polymerase subunit rpo7 (7 kDa, Protein from Vaccinia virus GLV-1h68)
- Core vaccinia dna-dependent rna polymerase complex (500 kDa, Complex)
- Dna-dependent rna polymerase subunits (Complex from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 35 kda subunit (35 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase subunit (21 kDa, Protein from Vaccinia virus GLV-1h68)
Structure of Vaccinia Virus DNA-dependent RNA polymerase elongation complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6rid
Q-score: 0.579
Hillen HS, Bartuli J, Grimm C, Dienemann C, Bedenk K, Szalay AA, Fischer U, Cramer P
Cell (2019) 179 pp. 1525- [ DOI: doi:10.1016/j.cell.2019.11.023 Pubmed: 31835031 ]
- Rna (9 kDa, RNA from Vaccinia virus)
- Dna-dependent rna polymerase subunit rpo147 (146 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase subunits (Complex from Vaccinia virus GLV-1h68)
- Dna-dependent rna polymerase subunit rpo18 (17 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 19 kda subunit (19 kDa, Protein from Vaccinia virus GLV-1h68)
- Magnesium ion (24 Da, Ligand)
- Nucleic acids (Complex from Vaccinia virus GLV-1h68)
- Dna-dependent rna polymerase subunit rpo132 (133 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-dependent rna polymerase subunit rpo132 (Complex from Vaccinia virus GLV-1h68)
- Vaccinia virus dna-dependent rna polymerase elongation complex (450 kDa, Complex)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase 30 kda polypeptide (29 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-dependent rna polymerase subunit rpo7 (7 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 35 kda subunit (35 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase subunit (21 kDa, Protein from Vaccinia virus GLV-1h68)
- Template strand dna (14 kDa, DNA from Vaccinia virus)
- Non-template strand dna (14 kDa, DNA from Vaccinia virus)
Structure of the complete Vaccinia DNA-dependent RNA polymerase complex
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 6rfl
Q-score: 0.572
Grimm C, Hillen HS, Bedenk K, Bartuli J, Neyer S, Zhang Q, Huttenhofer A, Erlacher M, Dienemann C, Schlosser A, Urlaub H, Bottcher B, Szalay AA, Cramer P, Fischer U
Cell (2019) 179 pp. 1537-1550.e19 [ DOI: doi:10.1016/j.cell.2019.11.024 Pubmed: 31835032 ]
- Dna-directed rna polymerase 19 kda subunit (19 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-dependent rna polymerase subunit rpo132 (133 kDa, Protein from Vaccinia virus GLV-1h68)
- Zinc ion (65 Da, Ligand)
- Dna-dependent rna polymerase subunit rpo7 (7 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase 35 kda subunit (35 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-directed rna polymerase subunit (21 kDa, Protein from Vaccinia virus GLV-1h68)
- Rna (Complex from Homo sapiens)
- Small subunit of mrna capping enzyme (33 kDa, Protein from Vaccinia virus GLV-1h68)
- Water (18 Da, Ligand)
- Dna-directed rna polymerase 30 kda polypeptide (30 kDa, Protein from Vaccinia virus GLV-1h68)
- Transcription factor vetf 82kda large subunit (82 kDa, Protein from Vaccinia virus GLV-1h68)
- Virion core protein (14 kDa, Protein from Vaccinia virus GLV-1h68)
- Dna-dependent rna polymerase subunit rpo18 (17 kDa, Protein from Vaccinia virus GLV-1h68)
- Putative h4l rna polymerase-associated transcription factor rap94 (93 kDa, Protein from Vaccinia virus GLV-1h68)
- Complete vaccinia dna-dependent rna polymerase complex (Complex)
- Dna-dependent rna polymerase subunit rpo147 (146 kDa, Protein from Vaccinia virus GLV-1h68)
- Magnesium ion (24 Da, Ligand)
- Vaccinia dna-dependent rna polymerase complex (Complex from Vaccinia virus GLV-1H68)
- Chr17.trna16-glnttg (23 kDa, RNA from Homo sapiens)
- Large subunit of mrna capping enzyme (96 kDa, Protein from Vaccinia virus GLV-1h68)
- Nucleoside triphosphate phosphohydrolase-i (72 kDa, Protein from Vaccinia virus GLV-1h68)