GltPh mutant (S279E/D405N) in complex with aspartate and sodium ions
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 7rcp
Q-score: 0.701
Reddy KD, Ciftci D, Scopelliti AJ, Boudker O
J Gen Physiol (2022) 154 [ DOI: doi:10.1085/jgp.202213131 Pubmed: 35452090 ]
- Water (18 Da, Ligand)
- Aspartic acid (133 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Complex of glutamate transporter homologue gltph mutant (s279e/d405n) with sodium nitrate and aspartate (Cellular component from Pyrococcus horikoshii)
- Glutamate transporter homolog (43 kDa, Protein from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3))
Inward-facing Apo-open state of the glutamate transporter homologue GltPh
Resolution: 3.52 Å
EM Method: Single-particle
Fitted PDBs: 6x12
Q-score: 0.506
eLife (2020) 9 [ Pubmed: 33155546 DOI: doi:10.7554/eLife.58417 ]
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
- Glutamate transporter homologue gltph (44 kDa, Protein from Pyrococcus horikoshii)
- Complex of inward-facing apo-open state of gltph in msp1e3 nanodisc (134 kDa, Complex from Pyrococcus horikoshii)
Inward-facing sodium-bound state of the glutamate transporter homologue GltPh
Resolution: 3.66 Å
EM Method: Single-particle
Fitted PDBs: 6x13
Q-score: 0.486
eLife (2020) 9 [ Pubmed: 33155546 DOI: doi:10.7554/eLife.58417 ]
- Complex of inward-facing sodium-bound state of gltph in msp1e3 nanodisc (134 kDa, Complex from Pyrococcus horikoshii)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
- Glutamate transporter homologue gltph (44 kDa, Protein from Pyrococcus horikoshii)
Inward-facing state of the glutamate transporter homologue GltPh in complex with TFB-TBOA
Resolution: 3.71 Å
EM Method: Single-particle
Fitted PDBs: 6x14
Q-score: 0.479
eLife (2020) 9 [ Pubmed: 33155546 DOI: doi:10.7554/eLife.58417 ]
- Complex of inward-facing state of gltph with tfb-tboa in msp1e3 nanodisc (134 kDa, Complex from Pyrococcus horikoshii)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
- Glutamate transporter homologue gltph (44 kDa, Protein from Pyrococcus horikoshii)
- (2~{s},3~{s})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid (426 Da, Ligand)
Resolution: 3.05 Å
EM Method: Single-particle
Fitted PDBs: 6x15
Q-score: 0.581
eLife (2020) 9 [ Pubmed: 33155546 DOI: doi:10.7554/eLife.58417 ]
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
- Water (18 Da, Ligand)
- Aspartic acid (133 Da, Ligand)
- Glutamate transporter homologue gltph (44 kDa, Protein from Pyrococcus horikoshii)
- Sodium ion (22 Da, Ligand)
- Complex of inward-facing state of gltph with l-aspartate and sodium ions in msp1e3 nanodisc (134 kDa, Complex from Pyrococcus horikoshii)
- Mercury (ii) ion (200 Da, Ligand)
Inward-facing state of the glutamate transporter homologue GltPh in complex with TBOA
Resolution: 3.39 Å
EM Method: Single-particle
Fitted PDBs: 6x16
Q-score: 0.516
eLife (2020) 9 [ Pubmed: 33155546 DOI: doi:10.7554/eLife.58417 ]
- Complex of inward-facing state of gltph with tboa in msp1e3 nanodisc (134 kDa, Complex from Pyrococcus horikoshii)
- (3s)-3-(benzyloxy)-l-aspartic acid (239 Da, Ligand)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
- Glutamate transporter homologue gltph (44 kDa, Protein from Pyrococcus horikoshii)
Outward-facing state of the glutamate transporter homologue GltPh in complex with TBOA
Resolution: 3.66 Å
EM Method: Single-particle
Fitted PDBs: 6x17
Q-score: 0.488
eLife (2020) 9 [ Pubmed: 33155546 DOI: doi:10.7554/eLife.58417 ]
- Water (18 Da, Ligand)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
- Glutamate transporter homologue gltph (44 kDa, Protein from Pyrococcus horikoshii)
- Complex of gltph with tboa in msp1e3 nanodisc (134 kDa, Complex from Pyrococcus horikoshii)
GltPh in complex with L-aspartate and sodium ions in outward-facing state
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 6uwf
Q-score: 0.553
Huang Y, Wang X, Lv G, Razavi AM, Huysmans GHM, Weinstein H, Bracken C, Eliezer D, Boudker O
Nat Chem Biol (2020) 16 pp. 1006-1012 [ DOI: doi:10.1038/s41589-020-0561-6 Pubmed: 32514183 ]
- Aspartic acid (133 Da, Ligand)
- Glutamate transporter homolog (44 kDa, Protein from Pyrococcus horikoshii)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
- Complex of gltph with l-aspartate and sodium ions in msp1e3 nanodisc (134 kDa, Complex from Pyrococcus horikoshii)
GltPh in complex with L-aspartate and sodium ions in intermediate outward-facing state
Resolution: 3.62 Å
EM Method: Single-particle
Fitted PDBs: 6uwl
Q-score: 0.454
Huang Y, Wang X, Lv G, Razavi AM, Huysmans GHM, Weinstein H, Bracken C, Eliezer D, Boudker O
Nat Chem Biol (2020) 16 pp. 1006-1012 [ DOI: doi:10.1038/s41589-020-0561-6 Pubmed: 32514183 ]
- Aspartic acid (133 Da, Ligand)
- Glutamate transporter homolog (44 kDa, Protein from Pyrococcus horikoshii)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
- Complex of gltph with l-aspartate and sodium ions in msp1e3 nanodisc (134 kDa, Complex from Pyrococcus horikoshii)