High resolution in-situ structure of complex I in respiratory supercomplex (composite)
Resolution: 2.1 Å
EM Method: Single-particle
Fitted PDBs: 8ud1
Q-score: 0.636
Zheng W, Chai P, Zhu J, Zhang K
Nature (2024) 631 pp. 232-239 [ DOI: doi:10.1038/s41586-024-07488-9 Pubmed: 38811722 ]
- ~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (14 kDa, Protein from Sus scrofa)
- In-situ cryo-em structure of respiratory supercomplex by directly imaging mitochondria (Complex from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit a13 (16 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (16 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (79 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (8 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit b2 (11 kDa, Protein from Sus scrofa)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4 (51 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (21 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from Sus scrofa)
- Potassium ion (39 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (8 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (40 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (21 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (11 kDa, Protein from Sus scrofa)
- Water (18 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit ab1 (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 (9 kDa, Protein from Sus scrofa)
- Flavin mononucleotide (456 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit a9 (42 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from Sus scrofa)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (30 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 isoform x1 (13 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from Sus scrofa)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (20 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (52 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (13 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (48 kDa, Protein from Sus scrofa)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (28 kDa, Protein from Sus scrofa)
- Cardiolipin (1 kDa, Ligand)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from Sus scrofa)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 (7 kDa, Protein from Sus scrofa)
- Myristic acid (228 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from Sus scrofa)
In-situ complex I (Active-Apo)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8ueo
Q-score: 0.417
Zheng W, Chai P, Zhu J, Zhang K
Nature (2024) 631 pp. 232-239 [ DOI: doi:10.1038/s41586-024-07488-9 Pubmed: 38811722 ]
- ~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from Sus scrofa)
- In-situ cryo-em structure of respiratory supercomplex by directly imaging mitochondria (Complex from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit a13 (16 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (16 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (79 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (49 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (8 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 3 (12 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit b2 (11 kDa, Protein from Sus scrofa)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (21 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from Sus scrofa)
- Potassium ion (39 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4 (51 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (8 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (40 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (21 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (11 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit ab1 (17 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 (9 kDa, Protein from Sus scrofa)
- Flavin mononucleotide (456 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit a9 (42 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from Sus scrofa)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (30 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 isoform x1 (13 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from Sus scrofa)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (20 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (13 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (48 kDa, Protein from Sus scrofa)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (28 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from Sus scrofa)
- Cardiolipin (1 kDa, Ligand)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 (7 kDa, Protein from Sus scrofa)
- Myristic acid (228 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from Sus scrofa)
In-situ complex I, Active-Q10 (State-alpha)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8uep
Q-score: 0.413
Zheng W, Chai P, Zhu J, Zhang K
Nature (2024) 631 pp. 232-239 [ DOI: doi:10.1038/s41586-024-07488-9 Pubmed: 38811722 ]
- ~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (54 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit a13 (16 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (16 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (79 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (8 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit b2 (11 kDa, Protein from Sus scrofa)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4 (51 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (21 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (30 kDa, Protein from Sus scrofa)
- Potassium ion (39 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (8 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (40 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (21 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 [sus scrofa] (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (11 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit ab1 (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 (9 kDa, Protein from Sus scrofa)
- Flavin mononucleotide (456 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit a9 (42 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from Sus scrofa)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 isoform x1 (13 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from Sus scrofa)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (20 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (13 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (48 kDa, Protein from Sus scrofa)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (28 kDa, Protein from Sus scrofa)
- Cardiolipin (1 kDa, Ligand)
- Complex i in respiratory supercomplex (Complex from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from Sus scrofa)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Ubiquinone-10 (863 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from Sus scrofa)
- Myristic acid (228 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from Sus scrofa)
In-situ complex I with Q10 (State-beta)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8ueq
Q-score: 0.366
Zheng W, Chai P, Zhu J, Zhang K
Nature (2024) 631 pp. 232-239 [ DOI: doi:10.1038/s41586-024-07488-9 Pubmed: 38811722 ]
- ~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (54 kDa, Protein from Sus scrofa)
- In-situ cryo-em structure of respiratory supercomplex by directly imaging mitochondria (Complex from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit a13 (16 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (16 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (79 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (8 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit b2 (11 kDa, Protein from Sus scrofa)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4 (51 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (21 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (30 kDa, Protein from Sus scrofa)
- Potassium ion (39 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (8 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (40 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (21 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 [sus scrofa] (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (11 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit ab1 (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 (9 kDa, Protein from Sus scrofa)
- Flavin mononucleotide (456 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit a9 (42 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from Sus scrofa)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 isoform x1 (13 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from Sus scrofa)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (20 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (13 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (48 kDa, Protein from Sus scrofa)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (28 kDa, Protein from Sus scrofa)
- Cardiolipin (1 kDa, Ligand)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from Sus scrofa)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Ubiquinone-10 (863 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from Sus scrofa)
- Myristic acid (228 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from Sus scrofa)
In-situ complex I with Q10 (State-gamma)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8uer
Q-score: 0.388
Zheng W, Chai P, Zhu J, Zhang K
Nature (2024) 631 pp. 232-239 [ DOI: doi:10.1038/s41586-024-07488-9 Pubmed: 38811722 ]
- ~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (54 kDa, Protein from Sus scrofa)
- In-situ cryo-em structure of respiratory supercomplex by directly imaging mitochondria (Complex from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit a13 (16 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (16 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (79 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (8 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit b2 (11 kDa, Protein from Sus scrofa)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4 (51 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (21 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (30 kDa, Protein from Sus scrofa)
- Potassium ion (39 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (8 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (40 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (21 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 [sus scrofa] (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (11 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit ab1 (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 (9 kDa, Protein from Sus scrofa)
- Flavin mononucleotide (456 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit a9 (42 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from Sus scrofa)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 isoform x1 (13 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from Sus scrofa)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (20 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (13 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (28 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (48 kDa, Protein from Sus scrofa)
- Iron/sulfur cluster (351 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from Sus scrofa)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Ubiquinone-10 (863 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from Sus scrofa)
- Myristic acid (228 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from Sus scrofa)
In-situ complex I, Deactive class01
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8ues
Q-score: 0.38
Zheng W, Chai P, Zhu J, Zhang K
Nature (2024) 631 pp. 232-239 [ DOI: doi:10.1038/s41586-024-07488-9 Pubmed: 38811722 ]
- ~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (54 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit a13 (16 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (16 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (79 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (8 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit b2 (11 kDa, Protein from Sus scrofa)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4 (51 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (21 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (30 kDa, Protein from Sus scrofa)
- Potassium ion (39 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (8 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (40 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (21 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 [sus scrofa] (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (11 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit ab1 (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 (9 kDa, Protein from Sus scrofa)
- Flavin mononucleotide (456 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit a9 (42 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from Sus scrofa)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 isoform x1 (13 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from Sus scrofa)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (20 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (13 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (48 kDa, Protein from Sus scrofa)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (28 kDa, Protein from Sus scrofa)
- Cardiolipin (1 kDa, Ligand)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from Sus scrofa)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from Sus scrofa)
- Myristic acid (228 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from Sus scrofa)
- In-situ cryo-em structure of respiratory supercomplex by directly imaging mitochondria (Cellular component from Sus scrofa)
In-situ complex I, Deactive class02
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8uet
Q-score: 0.377
Zheng W, Chai P, Zhu J, Zhang K
Nature (2024) 631 pp. 232-239 [ DOI: doi:10.1038/s41586-024-07488-9 Pubmed: 38811722 ]
- ~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (54 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit a13 (16 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (16 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (79 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (8 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit b2 (11 kDa, Protein from Sus scrofa)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4 (51 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (21 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (30 kDa, Protein from Sus scrofa)
- Potassium ion (39 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (8 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (40 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (21 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 [sus scrofa] (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (11 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit ab1 (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 (9 kDa, Protein from Sus scrofa)
- Flavin mononucleotide (456 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit a9 (42 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from Sus scrofa)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 isoform x1 (13 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from Sus scrofa)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (20 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (13 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (48 kDa, Protein from Sus scrofa)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (28 kDa, Protein from Sus scrofa)
- Cardiolipin (1 kDa, Ligand)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from Sus scrofa)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from Sus scrofa)
- Myristic acid (228 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from Sus scrofa)
- In-situ cryo-em structure of respiratory supercomplex by directly imaging mitochondria (Cellular component from Sus scrofa)
In-situ complex I, Deactive class03
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8ueu
Q-score: 0.371
Zheng W, Chai P, Zhu J, Zhang K
Nature (2024) 631 pp. 232-239 [ DOI: doi:10.1038/s41586-024-07488-9 Pubmed: 38811722 ]
- ~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (54 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit a13 (16 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (16 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (79 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (8 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit b2 (11 kDa, Protein from Sus scrofa)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4 (51 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (21 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (30 kDa, Protein from Sus scrofa)
- Potassium ion (39 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (8 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (40 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (21 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 [sus scrofa] (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (11 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit ab1 (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 (9 kDa, Protein from Sus scrofa)
- Flavin mononucleotide (456 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit a9 (42 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from Sus scrofa)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 isoform x1 (13 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from Sus scrofa)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (20 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (13 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (48 kDa, Protein from Sus scrofa)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (28 kDa, Protein from Sus scrofa)
- Cardiolipin (1 kDa, Ligand)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from Sus scrofa)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from Sus scrofa)
- Myristic acid (228 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from Sus scrofa)
- In-situ cryo-em structure of respiratory supercomplex by directly imaging mitochondria (Cellular component from Sus scrofa)
In-situ complex I, Deactive class04
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8uev
Q-score: 0.372
Zheng W, Chai P, Zhu J, Zhang K
Nature (2024) 631 pp. 232-239 [ DOI: doi:10.1038/s41586-024-07488-9 Pubmed: 38811722 ]
- ~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (54 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit a13 (16 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (16 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (79 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (8 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit b2 (11 kDa, Protein from Sus scrofa)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4 (51 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (21 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (30 kDa, Protein from Sus scrofa)
- Potassium ion (39 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (8 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (40 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (21 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 [sus scrofa] (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (11 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit ab1 (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 (9 kDa, Protein from Sus scrofa)
- Flavin mononucleotide (456 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit a9 (42 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from Sus scrofa)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 isoform x1 (13 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from Sus scrofa)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (20 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (13 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (48 kDa, Protein from Sus scrofa)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (28 kDa, Protein from Sus scrofa)
- Cardiolipin (1 kDa, Ligand)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from Sus scrofa)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from Sus scrofa)
- Myristic acid (228 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from Sus scrofa)
- In-situ cryo-em structure of respiratory supercomplex by directly imaging mitochondria (Cellular component from Sus scrofa)
In-situ complex I, Deactive class05
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8uew
Q-score: 0.379
Zheng W, Chai P, Zhu J, Zhang K
Nature (2024) 631 pp. 232-239 [ DOI: doi:10.1038/s41586-024-07488-9 Pubmed: 38811722 ]
- ~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (54 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit a13 (16 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (16 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (79 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (8 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit b2 (11 kDa, Protein from Sus scrofa)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4 (51 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (21 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (30 kDa, Protein from Sus scrofa)
- Potassium ion (39 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (8 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (40 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (21 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 [sus scrofa] (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (11 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit ab1 (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 (9 kDa, Protein from Sus scrofa)
- Flavin mononucleotide (456 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit a9 (42 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from Sus scrofa)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 isoform x1 (13 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from Sus scrofa)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (20 kDa, Protein from Sus scrofa)
- In-situ cryo-em structure of respiratory supercomplex by directly imaging mitochondriain-situ cryo-em structure of respiratory supercomplex by directly imaging mitochondria (Complex from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (13 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (48 kDa, Protein from Sus scrofa)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (28 kDa, Protein from Sus scrofa)
- Cardiolipin (1 kDa, Ligand)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from Sus scrofa)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from Sus scrofa)
- Myristic acid (228 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from Sus scrofa)