Cryo-EM structure of the dynactin complex at 3.8 Angstrom resolution
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6znl
Q-score: 0.352
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ DOI: doi:10.15252/embj.2020106164 Pubmed: 33734450 ]
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from Sus scrofa)
- F-actin capping protein beta subunit (30 kDa, Protein from Sus scrofa)
- Actin, cytoplasmic 1 (41 kDa, Protein from Sus scrofa)
- Dynactin complex (1 MDa, Complex from Sus scrofa)
- Dynactin subunit 5 (20 kDa, Protein from Sus scrofa)
- Dynactin subunit 3 (21 kDa, Protein from Sus scrofa)
- Dynactin subunit 2 (44 kDa, Protein from Sus scrofa)
- Dynactin 6 (20 kDa, Protein from Sus scrofa)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dynactin subunit 4 (52 kDa, Protein from Sus scrofa)
- Dynactin subunit 1 (142 kDa, Protein from Sus scrofa)
- Arp11 (46 kDa, Protein from Sus scrofa)
- Capping protein (actin filament) muscle z-line, alpha 1 (33 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Zinc ion (65 Da, Ligand)
The shoulder domain of dynactin with underlying Arp1 filament subnits
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ DOI: doi:10.15252/embj.2020106164 Pubmed: 33734450 ]
- Dynactin complex (1 MDa, Complex from Sus scrofa)
The pointed end complex of dynactin, with accompanying Arp1/actin filament subunits
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ DOI: doi:10.15252/embj.2020106164 Pubmed: 33734450 ]
- Dynactin complex (1 MDa, Complex from Sus scrofa)
Map of the shoulder domain from dynactin to resolve the upper paddle and hook regions
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ DOI: doi:10.15252/embj.2020106164 Pubmed: 33734450 ]
- Dynactin complex (1 MDa, Complex from Sus scrofa)
The pointed end complex of dynactin bound to BICDR1
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6znm
Q-score: 0.34
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ DOI: doi:10.15252/embj.2020106164 Pubmed: 33734450 ]
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from Sus scrofa)
- Dynactin subunit 5 (20 kDa, Protein from Sus scrofa)
- Actin, cytoplasmic 1 (41 kDa, Protein from Sus scrofa)
- Dynactin subunit 2 (44 kDa, Protein from Sus scrofa)
- Dynactin 6 (20 kDa, Protein from Sus scrofa)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dynactin subunit 4 (52 kDa, Protein from Sus scrofa)
- Dynein-dynactin-bicdr1 complex (1 MDa, Complex)
- Arp11 (46 kDa, Protein from Sus scrofa)
- Dynactin (Complex from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Bicdr1 (Complex from Mus musculus)
- Zinc ion (65 Da, Ligand)
- Bicd family-like cargo adapter 1,bicd family-like cargo adapter 1,bicdr1 (58 kDa, Protein from Mus musculus)
The pointed end complex of dynactin bound to Hook3
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6znn
Q-score: 0.264
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ DOI: doi:10.15252/embj.2020106164 Pubmed: 33734450 ]
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from Sus scrofa)
- Hook3 (Complex from Homo sapiens)
- Actin, cytoplasmic 1 (41 kDa, Protein from Sus scrofa)
- Putative hook3 coiled coil (5 kDa, Protein from Homo sapiens)
- Dynactin subunit 5 (20 kDa, Protein from Sus scrofa)
- Dynactin subunit 2 (44 kDa, Protein from Sus scrofa)
- Dynactin 6 (20 kDa, Protein from Sus scrofa)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Hook3 (7 kDa, Protein from Homo sapiens)
- Dynactin subunit 4 (52 kDa, Protein from Sus scrofa)
- Arp11 (46 kDa, Protein from Sus scrofa)
- Dynactin (Complex from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Hook3 (7 kDa, Protein from Homo sapiens)
- Dynein-dynactin-hook3 complex (Complex)
- Zinc ion (65 Da, Ligand)
The pointed end complex of dynactin with the p150 projection docked
Resolution: 6.8 Å
EM Method: Single-particle
Fitted PDBs: 6zno
Q-score: 0.158
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ DOI: doi:10.15252/embj.2020106164 Pubmed: 33734450 ]
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from Sus scrofa)
- Dynactin subunit 5 (20 kDa, Protein from Sus scrofa)
- Actin, cytoplasmic 1 (41 kDa, Protein from Sus scrofa)
- Dynactin subunit 1 (16 kDa, Protein from Sus scrofa)
- Dynactin 6 (18 kDa, Protein from Sus scrofa)
- Dynactin subunit 2 (44 kDa, Protein from Sus scrofa)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dynactin subunit 4 (52 kDa, Protein from Sus scrofa)
- Arp11 (46 kDa, Protein from Sus scrofa)
- Dynactin (Complex from Sus scrofa)
- Dynactin subunit 1 (16 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Zinc ion (65 Da, Ligand)
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6rza
Q-score: 0.228
Lacey SE, He S, Scheres SH, Carter AP
eLife (2019) 8 [ DOI: doi:10.7554/eLife.47145 Pubmed: 31264960 ]
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
- Tubulin (Complex from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha-1b chain (48 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Cytoplasmic dynein 1 heavy chain 1,dynein heavy chain 7, axonemal,cytoplasmic dynein 1 heavy chain 1 (18 kDa, Protein from Mus musculus)
- Dnah7 (Complex from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Taxol (853 Da, Ligand)
- Dnah7 microtubule binding domain bound to microtubules (Complex)
Cryo-EM structure of mouse cytoplasmic dynein-1 microtubule binding domain bound to microtubules
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6rzb
Q-score: 0.324
Lacey SE, He S, Scheres SH, Carter AP
eLife (2019) 8 [ DOI: doi:10.7554/eLife.47145 Pubmed: 31264960 ]
- Dynein-1 microtubule binding (Complex from Mus musculus)
- Mkiaa0325 protein (16 kDa, Protein from Mus musculus)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Mouse cytoplasmic dynein-1 microtubule binding domain decorating microtubules (Complex)
- Tubulin alpha-1b chain (48 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Taxol (853 Da, Ligand)
- Tubulin (Complex from Sus scrofa)
Human cytoplasmic dynein-1 bound to dynactin and an N-terminal construct of BICD2
Resolution: 8.7 Å
EM Method: Single-particle
Fitted PDBs: 5nw4
Q-score: 0.113
Zhang K, Foster HE, Rondelet A, Lacey SE, Bahi-Buisson N, Bird AW, Carter AP
Cell (2017) 169 pp. 1303-1314.e18 [ DOI: doi:10.1016/j.cell.2017.05.025 Pubmed: 28602352 ]
- Dynein light intermediate chain (25 kDa, Protein from Homo sapiens)
- F-actin capping protein beta subunit variant ii (33 kDa, Protein from Sus scrofa)
- Dynactin (3 kDa, Protein from Sus scrofa)
- Bicd2 (Complex from Homo sapiens)
- Capping protein (actin filament) muscle z-line, alpha 1 (33 kDa, Protein from Sus scrofa)
- P150 (4 kDa, Protein from Sus scrofa)
- Dynactin p150 (4 kDa, Protein from Sus scrofa)
- Dynactin shoulder complex (11 kDa, Protein from Sus scrofa)
- Dynactin shoulder complex (51 kDa, Protein from Sus scrofa)
- Dynein intermediate chain (29 kDa, Protein from Homo sapiens)
- Dynactin (Complex from Sus scrofa)
- Dynein-1 (Complex from Homo sapiens)
- Dynactin 6 (20 kDa, Protein from Sus scrofa)
- Dynactin shoulder complex (48 kDa, Protein from Sus scrofa)
- Dynactin (2 kDa, Protein from Sus scrofa)
- Arp11 (46 kDa, Protein from Sus scrofa)
- Dynactin (7 kDa, Protein from Sus scrofa)
- Dynactin (5 kDa, Protein from Sus scrofa)
- Dynein heavy chain (75 kDa, Protein from Homo sapiens)
- Dynein n-terminal dimerization domain (10 kDa, Protein from Homo sapiens)
- Human cytoplasmic dynein-1 bound to dynactin and an n-terminal construct of bicd2 (2 MDa, Complex)
- Beta-actin (43 kDa, Protein from Sus scrofa)
- Dynactin pointed end p62 (20 kDa, Protein from Sus scrofa)
- Dynactin subunit 5 (24 kDa, Protein from Sus scrofa)
- Bicd2n (23 kDa, Protein from Homo sapiens)
- Arp1 (42 kDa, Protein from Sus scrofa)
- Dynein n-terminal dimerization domain (10 kDa, Protein from Homo sapiens)
- Robl (10 kDa, Protein from Homo sapiens)
- Dynein heavy chain (78 kDa, Protein from Homo sapiens)
- Dynactin shoulder complex (14 kDa, Protein from Sus scrofa)
- Dynein light intermediate chain (25 kDa, Protein from Homo sapiens)
- Dynein intermediate chain (29 kDa, Protein from Homo sapiens)