CryoEM Density of Acetylated Microtubules
Resolution: 3.7 Å
EM Method: Helical reconstruction
Fitted PDBs: 6o2q
Q-score: 0.428
Eshun-Wilson L, Zhang R, Portran D, Nachury MV, Toso DB, Lohr T, Vendruscolo M, Bonomi M, Fraser JS, Nogales E
PNAS (2019) 116 pp. 10366-10371 [ DOI: doi:10.1073/pnas.1900441116 Pubmed: 31072936 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Acetylated microtubule (Tissue from Sus scrofa)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
Resolution: 3.3 Å
EM Method: Helical reconstruction
Fitted PDBs: 6o2r
Q-score: 0.461
Eshun-Wilson L, Zhang R, Portran D, Nachury MV, Toso DB, Lohr T, Vendruscolo M, Bonomi M, Fraser JS, Nogales E
PNAS (2019) 116 pp. 10366-10371 [ DOI: doi:10.1073/pnas.1900441116 Pubmed: 31072936 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Deacetylated microtubule (Tissue from Sus scrofa)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
Resolution: 4.0 Å
EM Method: Helical reconstruction
Fitted PDBs: 6o2s
Q-score: 0.275
Eshun-Wilson L, Zhang R, Portran D, Nachury MV, Toso DB, Lohr T, Vendruscolo M, Bonomi M, Fraser JS, Nogales E
PNAS (2019) 116 pp. 10366-10371 [ DOI: doi:10.1073/pnas.1900441116 Pubmed: 31072936 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Deacetylated microtubule (Tissue from Sus scrofa)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
Resolution: 4.1 Å
EM Method: Helical reconstruction
Fitted PDBs: 6o2t
Q-score: 0.252
Eshun-Wilson L, Zhang R, Portran D, Nachury MV, Toso DB, Lohr T, Vendruscolo M, Bonomi M, Fraser JS, Nogales E
PNAS (2019) 116 pp. 10366-10371 [ DOI: doi:10.1073/pnas.1900441116 Pubmed: 31072936 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Acetylated microtubule (Tissue from Sus scrofa)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
Undecorated GMPCPP microtubule
Resolution: 3.1 Å
EM Method: Helical reconstruction
Fitted PDBs: 6dpu
Q-score: 0.494
Zhang R, LaFrance B, Nogales E
PNAS (2018) 115 pp. E6191-E6200 [ DOI: doi:10.1073/pnas.1802637115 Pubmed: 29915050 ]
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Microtubule stabilized by gmpcpp (Complex from Sus scrofa)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
Resolution: 3.3 Å
EM Method: Helical reconstruction
Fitted PDBs: 6dpv
Q-score: 0.486
Zhang R, LaFrance B, Nogales E
PNAS (2018) 115 pp. E6191-E6200 [ DOI: doi:10.1073/pnas.1802637115 Pubmed: 29915050 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Dynamic microtubule in gdp state (Complex from Sus scrofa)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
Undecorated GTPgammaS microtubule
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 6dpw
Q-score: 0.456
Zhang R, LaFrance B, Nogales E
PNAS (2018) 115 pp. E6191-E6200 [ DOI: doi:10.1073/pnas.1802637115 Pubmed: 29915050 ]
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Gtpgammas-microtubule polymerized from gmpcpp-microtubule seeds (Complex from Sus scrofa)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 5'-guanosine-diphosphate-monothiophosphate (539 Da, Ligand)
Preformed GMPCPP microtubule washed with EB3, class 1
Zhang R, LaFrance B, Nogales E
PNAS (2018) 115 pp. E6191-E6200 [ DOI: doi:10.1073/pnas.1802637115 Pubmed: 29915050 ]
- Alpha tubulin (Protein from Sus scrofa)
- Preformed gmpcpp microtubule washed with eb3, class 1 (Cellular component)
- Beta tubulin (Protein from Sus scrofa)
- Eb3 (Protein from Homo sapiens)
- Eb3 (Complex from Homo sapiens)
- Microtubule (Cellular component from Sus scrofa)
Preformed GMPCPP microtubule washed with EB3, class 2
Zhang R, LaFrance B, Nogales E
PNAS (2018) 115 pp. E6191-E6200 [ DOI: doi:10.1073/pnas.1802637115 Pubmed: 29915050 ]
- Eb3 (Complex from Homo sapiens)
- Microtubule (Cellular component from Sus scrofa)
- Eb3 (Protein from Homo sapiens)
- Beta tubulin (Protein from Sus scrofa)
- Preformed gmpcpp microtubule washed with eb3, class 2 (Cellular component)
- Alpha tubulin (Protein from Sus scrofa)