Cryo-EM structure of microtubule-bound KLP61F motor domain in the AMPPNP state
Resolution: 4.4 Å
EM Method: Helical reconstruction
Fitted PDBs: 6vpo
Q-score: 0.324
Bodrug T, Wilson-Kubalek EM, Nithianantham S, Thompson AF, Alfieri A, Gaska I, Major J, Debs G, Inagaki S, Gutierrez P, Gheber L, McKenney RJ, Sindelar CV, Milligan R, Stumpff J, Rosenfeld SS, Forth ST, Al-Bassam J
eLife (2020) 9 [ DOI: doi:10.7554/eLife.51131 Pubmed: 31958056 ]
- Microtubule (Complex from Sus scrofa)
- Klp61f motor (Complex from Drosophila melanogaster)
- Kinesin-like protein klp61f (42 kDa, Protein from Drosophila melanogaster)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Microtubule-bound klp61f motor in the amppnp state (Complex)
- Tubulin alpha-1a chain (50 kDa, Protein from Sus scrofa)
Cryo-EM structure of microtubule-bound KLP61F motor with tail domain in the nucleotide-free state
Resolution: 4.4 Å
EM Method: Helical reconstruction
Fitted PDBs: 6vpp
Q-score: 0.218
Bodrug T, Wilson-Kubalek EM, Nithianantham S, Thompson AF, Alfieri A, Gaska I, Major J, Debs G, Inagaki S, Gutierrez P, Gheber L, McKenney RJ, Sindelar CV, Milligan R, Stumpff J, Rosenfeld SS, Forth ST, Al-Bassam J
eLife (2020) 9 [ DOI: doi:10.7554/eLife.51131 Pubmed: 31958056 ]
- Microtubule (Complex from Sus scrofa)
- Kinesin-like protein klp61f (42 kDa, Protein from Drosophila melanogaster)
- Magnesium ion (24 Da, Ligand)
- Microtubule-bound klp61f motor with tail domain in the nucleotide-free state (Complex)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Klp61f motor (Complex from Drosophila melanogaster)
- Tubulin alpha-1a chain (50 kDa, Protein from Sus scrofa)
KLP10A-AMPPNP in complex with curved tubulin and a microtubule
Resolution: 3.51 Å
EM Method: Helical reconstruction
Fitted PDBs: 6b0c
Q-score: 0.436
Benoit MPMH, Asenjo AB, Sosa H
Nat Commun (2018) 9 pp. 1662-1662 [ DOI: doi:10.1038/s41467-018-04044-8 Pubmed: 29695795 ]
- Klp10a-amppnp motor in complex with curved tubulin and a microtubule (Complex)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Kinesin-like protein klp10a (41 kDa, Protein from Drosophila melanogaster)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Taxol (853 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Klp10a-amppnp motor (Complex from Drosophila melanogaster)
- Microtubule (Cellular component from Sus scrofa)
Apo KLP10A in complex with a microtubule
Resolution: 3.78 Å
EM Method: Helical reconstruction
Fitted PDBs: 6b0i
Q-score: 0.433
Benoit MPMH, Asenjo AB, Sosa H
Nat Commun (2018) 9 pp. 1662-1662 [ DOI: doi:10.1038/s41467-018-04044-8 Pubmed: 29695795 ]
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Taxol (853 Da, Ligand)
- Apo-klp10a neck-motor (Complex from Drosophila melanogaster)
- Apo-klp10a neck-motor in complex with a microtubule (Complex)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Kinesin-like protein klp10a (46 kDa, Protein from Drosophila melanogaster)
- Microtubule (Cellular component from Sus scrofa)
KLP10A-AMPPNP in complex with a microtubule
Resolution: 3.98 Å
EM Method: Helical reconstruction
Fitted PDBs: 6b0l
Q-score: 0.314
Benoit MPMH, Asenjo AB, Sosa H
Nat Commun (2018) 9 pp. 1662-1662 [ DOI: doi:10.1038/s41467-018-04044-8 Pubmed: 29695795 ]
- Klp10a-amppmp (Complex from Drosophila melanogaster)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Taxol (853 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Klp10a-amppmp in complex with a microtubule (Complex)
- Kinesin-like protein klp10a (46 kDa, Protein from Drosophila melanogaster)
- Microtubule (Cellular component from Sus scrofa)