Cryo-EM structure of microtubule-bound KLP61F motor domain in the AMPPNP state
Resolution: 4.4 Å
EM Method: Helical reconstruction
Fitted PDBs: 6vpo
Q-score: 0.324
Bodrug T, Wilson-Kubalek EM, Nithianantham S, Thompson AF, Alfieri A, Gaska I, Major J, Debs G, Inagaki S, Gutierrez P, Gheber L, McKenney RJ, Sindelar CV, Milligan R, Stumpff J, Rosenfeld SS, Forth ST, Al-Bassam J
eLife (2020) 9 [ DOI: doi:10.7554/eLife.51131 Pubmed: 31958056 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Tubulin alpha-1a chain (50 kDa, Protein from Sus scrofa)
- Microtubule (Complex from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Kinesin-like protein klp61f (42 kDa, Protein from Drosophila melanogaster)
- Microtubule-bound klp61f motor in the amppnp state (Complex)
- Klp61f motor (Complex from Drosophila melanogaster)
Cryo-EM structure of microtubule-bound KLP61F motor with tail domain in the nucleotide-free state
Resolution: 4.4 Å
EM Method: Helical reconstruction
Fitted PDBs: 6vpp
Q-score: 0.218
Bodrug T, Wilson-Kubalek EM, Nithianantham S, Thompson AF, Alfieri A, Gaska I, Major J, Debs G, Inagaki S, Gutierrez P, Gheber L, McKenney RJ, Sindelar CV, Milligan R, Stumpff J, Rosenfeld SS, Forth ST, Al-Bassam J
eLife (2020) 9 [ DOI: doi:10.7554/eLife.51131 Pubmed: 31958056 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Tubulin alpha-1a chain (50 kDa, Protein from Sus scrofa)
- Microtubule (Complex from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Kinesin-like protein klp61f (42 kDa, Protein from Drosophila melanogaster)
- Microtubule-bound klp61f motor with tail domain in the nucleotide-free state (Complex)
- Klp61f motor (Complex from Drosophila melanogaster)
KLP10A-AMPPNP in complex with curved tubulin and a microtubule
Resolution: 3.51 Å
EM Method: Helical reconstruction
Fitted PDBs: 6b0c
Q-score: 0.436
Benoit MPMH, Asenjo AB, Sosa H
Nat Commun (2018) 9 pp. 1662-1662 [ Pubmed: 29695795 DOI: doi:10.1038/s41467-018-04044-8 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Taxol (853 Da, Ligand)
- Klp10a-amppnp motor in complex with curved tubulin and a microtubule (Complex)
- Klp10a-amppnp motor (Complex from Drosophila melanogaster)
- Microtubule (Cellular component from Sus scrofa)
- Kinesin-like protein klp10a (41 kDa, Protein from Drosophila melanogaster)
Apo KLP10A in complex with a microtubule
Resolution: 3.78 Å
EM Method: Helical reconstruction
Fitted PDBs: 6b0i
Q-score: 0.433
Benoit MPMH, Asenjo AB, Sosa H
Nat Commun (2018) 9 pp. 1662-1662 [ Pubmed: 29695795 DOI: doi:10.1038/s41467-018-04044-8 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Kinesin-like protein klp10a (46 kDa, Protein from Drosophila melanogaster)
- Magnesium ion (24 Da, Ligand)
- Apo-klp10a neck-motor (Complex from Drosophila melanogaster)
- Apo-klp10a neck-motor in complex with a microtubule (Complex)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Taxol (853 Da, Ligand)
- Microtubule (Cellular component from Sus scrofa)
KLP10A-AMPPNP in complex with a microtubule
Resolution: 3.98 Å
EM Method: Helical reconstruction
Fitted PDBs: 6b0l
Q-score: 0.314
Benoit MPMH, Asenjo AB, Sosa H
Nat Commun (2018) 9 pp. 1662-1662 [ Pubmed: 29695795 DOI: doi:10.1038/s41467-018-04044-8 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Kinesin-like protein klp10a (46 kDa, Protein from Drosophila melanogaster)
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Klp10a-amppmp in complex with a microtubule (Complex)
- Klp10a-amppmp (Complex from Drosophila melanogaster)
- Taxol (853 Da, Ligand)
- Microtubule (Cellular component from Sus scrofa)