Cryo-EM structure of the dynactin complex at 3.8 Angstrom resolution
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6znl
Q-score: 0.352
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ DOI: doi:10.15252/embj.2020106164 Pubmed: 33734450 ]
- Dynactin subunit 2 (44 kDa, Protein from Sus scrofa)
- Dynactin 6 (20 kDa, Protein from Sus scrofa)
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dynactin subunit 4 (52 kDa, Protein from Sus scrofa)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Capping protein (actin filament) muscle z-line, alpha 1 (33 kDa, Protein from Sus scrofa)
- Dynactin subunit 5 (20 kDa, Protein from Sus scrofa)
- Dynactin subunit 1 (142 kDa, Protein from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- F-actin capping protein beta subunit (30 kDa, Protein from Sus scrofa)
- Actin, cytoplasmic 1 (41 kDa, Protein from Sus scrofa)
- Dynactin complex (1 MDa, Complex from Sus scrofa)
- Dynactin subunit 3 (21 kDa, Protein from Sus scrofa)
- Arp11 (46 kDa, Protein from Sus scrofa)
The pointed end complex of dynactin bound to BICDR1
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6znm
Q-score: 0.34
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ DOI: doi:10.15252/embj.2020106164 Pubmed: 33734450 ]
- Bicdr1 (Complex from Mus musculus)
- Dynactin subunit 2 (44 kDa, Protein from Sus scrofa)
- Dynactin 6 (20 kDa, Protein from Sus scrofa)
- Bicd family-like cargo adapter 1,bicd family-like cargo adapter 1,bicdr1 (58 kDa, Protein from Mus musculus)
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from Sus scrofa)
- Dynein-dynactin-bicdr1 complex (1 MDa, Complex)
- Dynactin subunit 4 (52 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dynactin subunit 5 (20 kDa, Protein from Sus scrofa)
- Arp11 (46 kDa, Protein from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Actin, cytoplasmic 1 (41 kDa, Protein from Sus scrofa)
- Dynactin (Complex from Sus scrofa)
The pointed end complex of dynactin bound to Hook3
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6znn
Q-score: 0.264
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ DOI: doi:10.15252/embj.2020106164 Pubmed: 33734450 ]
- Dynactin (Complex from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Arp11 (46 kDa, Protein from Sus scrofa)
- Dynactin subunit 2 (44 kDa, Protein from Sus scrofa)
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from Sus scrofa)
- Actin, cytoplasmic 1 (41 kDa, Protein from Sus scrofa)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Putative hook3 coiled coil (5 kDa, Protein from Homo sapiens)
- Hook3 (Complex from Homo sapiens)
- Dynein-dynactin-hook3 complex (Complex)
- Dynactin 6 (20 kDa, Protein from Sus scrofa)
- Dynactin subunit 5 (20 kDa, Protein from Sus scrofa)
- Dynactin subunit 4 (52 kDa, Protein from Sus scrofa)
- Hook3 (7 kDa, Protein from Homo sapiens)
- Hook3 (7 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
The pointed end complex of dynactin with the p150 projection docked
Resolution: 6.8 Å
EM Method: Single-particle
Fitted PDBs: 6zno
Q-score: 0.158
Lau CK, O'Reilly FJ, Santhanam B, Lacey SE, Rappsilber J, Carter AP
EMBO J (2021) 40 pp. e106164-e106164 [ DOI: doi:10.15252/embj.2020106164 Pubmed: 33734450 ]
- Dynactin subunit 2 (44 kDa, Protein from Sus scrofa)
- Dynactin (Complex from Sus scrofa)
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from Sus scrofa)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dynactin subunit 4 (52 kDa, Protein from Sus scrofa)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dynactin 6 (18 kDa, Protein from Sus scrofa)
- Dynactin subunit 5 (20 kDa, Protein from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Dynactin subunit 1 (16 kDa, Protein from Sus scrofa)
- Actin, cytoplasmic 1 (41 kDa, Protein from Sus scrofa)
- Arp11 (46 kDa, Protein from Sus scrofa)
- Dynactin subunit 1 (16 kDa, Protein from Sus scrofa)
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6rza
Q-score: 0.228
Lacey SE, He S, Scheres SH, Carter AP
eLife (2019) 8 [ DOI: doi:10.7554/eLife.47145 Pubmed: 31264960 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin alpha-1b chain (48 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Dnah7 microtubule binding domain bound to microtubules (Complex)
- Tubulin (Complex from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Cytoplasmic dynein 1 heavy chain 1,dynein heavy chain 7, axonemal,cytoplasmic dynein 1 heavy chain 1 (18 kDa, Protein from Mus musculus)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
- Dnah7 (Complex from Homo sapiens)
- Taxol (853 Da, Ligand)
Cryo-EM structure of mouse cytoplasmic dynein-1 microtubule binding domain bound to microtubules
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6rzb
Q-score: 0.324
Lacey SE, He S, Scheres SH, Carter AP
eLife (2019) 8 [ DOI: doi:10.7554/eLife.47145 Pubmed: 31264960 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin alpha-1b chain (48 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Taxol (853 Da, Ligand)
- Mouse cytoplasmic dynein-1 microtubule binding domain decorating microtubules (Complex)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Dynein-1 microtubule binding (Complex from Mus musculus)
- Mkiaa0325 protein (16 kDa, Protein from Mus musculus)
- Tubulin (Complex from Sus scrofa)
Human cytoplasmic dynein-1 bound to dynactin and an N-terminal construct of BICD2
Resolution: 8.7 Å
EM Method: Single-particle
Fitted PDBs: 5nw4
Q-score: 0.113
Zhang K, Foster HE, Rondelet A, Lacey SE, Bahi-Buisson N, Bird AW, Carter AP
Cell (2017) 169 pp. 1303-1314.e18 [ Pubmed: 28602352 DOI: doi:10.1016/j.cell.2017.05.025 ]
- Dynactin (Complex from Sus scrofa)
- Dynein n-terminal dimerization domain (10 kDa, Protein from Homo sapiens)
- Dynactin pointed end p62 (20 kDa, Protein from Sus scrofa)
- Bicd2n (23 kDa, Protein from Homo sapiens)
- Dynactin (2 kDa, Protein from Sus scrofa)
- Dynactin shoulder complex (14 kDa, Protein from Sus scrofa)
- Bicd2 (Complex from Homo sapiens)
- Dynactin shoulder complex (11 kDa, Protein from Sus scrofa)
- Dynactin (3 kDa, Protein from Sus scrofa)
- Robl (10 kDa, Protein from Homo sapiens)
- Capping protein (actin filament) muscle z-line, alpha 1 (33 kDa, Protein from Sus scrofa)
- Dynactin p150 (4 kDa, Protein from Sus scrofa)
- Dynein intermediate chain (29 kDa, Protein from Homo sapiens)
- Arp11 (46 kDa, Protein from Sus scrofa)
- Dynein heavy chain (78 kDa, Protein from Homo sapiens)
- Dynein light intermediate chain (25 kDa, Protein from Homo sapiens)
- Dynein-1 (Complex from Homo sapiens)
- Dynactin 6 (20 kDa, Protein from Sus scrofa)
- Dynactin (5 kDa, Protein from Sus scrofa)
- Dynein heavy chain (75 kDa, Protein from Homo sapiens)
- Human cytoplasmic dynein-1 bound to dynactin and an n-terminal construct of bicd2 (2 MDa, Complex)
- P150 (4 kDa, Protein from Sus scrofa)
- Dynactin subunit 5 (24 kDa, Protein from Sus scrofa)
- Dynactin (7 kDa, Protein from Sus scrofa)
- Dynactin shoulder complex (48 kDa, Protein from Sus scrofa)
- Dynactin shoulder complex (51 kDa, Protein from Sus scrofa)
- Arp1 (42 kDa, Protein from Sus scrofa)
- F-actin capping protein beta subunit variant ii (33 kDa, Protein from Sus scrofa)
- Dynein intermediate chain (29 kDa, Protein from Homo sapiens)
- Dynein n-terminal dimerization domain (10 kDa, Protein from Homo sapiens)
- Beta-actin (43 kDa, Protein from Sus scrofa)
- Dynein light intermediate chain (25 kDa, Protein from Homo sapiens)