Cryo-EM structure of microtubule-bound KLP61F motor domain in the AMPPNP state
Resolution: 4.4 Å
EM Method: Helical reconstruction
Fitted PDBs: 6vpo
Q-score: 0.324
Bodrug T, Wilson-Kubalek EM, Nithianantham S, Thompson AF, Alfieri A, Gaska I, Major J, Debs G, Inagaki S, Gutierrez P, Gheber L, McKenney RJ, Sindelar CV, Milligan R, Stumpff J, Rosenfeld SS, Forth ST, Al-Bassam J
eLife (2020) 9 [ DOI: doi:10.7554/eLife.51131 Pubmed: 31958056 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Tubulin alpha-1a chain (50 kDa, Protein from Sus scrofa)
- Microtubule (Complex from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Kinesin-like protein klp61f (42 kDa, Protein from Drosophila melanogaster)
- Microtubule-bound klp61f motor in the amppnp state (Complex)
- Klp61f motor (Complex from Drosophila melanogaster)
Cryo-EM structure of microtubule-bound KLP61F motor with tail domain in the nucleotide-free state
Resolution: 4.4 Å
EM Method: Helical reconstruction
Fitted PDBs: 6vpp
Q-score: 0.218
Bodrug T, Wilson-Kubalek EM, Nithianantham S, Thompson AF, Alfieri A, Gaska I, Major J, Debs G, Inagaki S, Gutierrez P, Gheber L, McKenney RJ, Sindelar CV, Milligan R, Stumpff J, Rosenfeld SS, Forth ST, Al-Bassam J
eLife (2020) 9 [ DOI: doi:10.7554/eLife.51131 Pubmed: 31958056 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Tubulin alpha-1a chain (50 kDa, Protein from Sus scrofa)
- Microtubule (Complex from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Kinesin-like protein klp61f (42 kDa, Protein from Drosophila melanogaster)
- Microtubule-bound klp61f motor with tail domain in the nucleotide-free state (Complex)
- Klp61f motor (Complex from Drosophila melanogaster)
Tubulin cofactors-D,E and Arl2-Q73L GTPase form a stable heterotrimeric chaperone
Nithianantham S, Le S, Seto E, Jia W, Leary J, Corbett KD, Moore JK, Al-Bassam J
eLife (2015) 4 [ Pubmed: 26208336 DOI: doi:10.7554/eLife.08811 ]
Tubulin cofactors-D,E(N-GFP) and Arl2-Q73L GTPase form a stable heterotrimeric chaperone
Nithianantham S, Le S, Seto E, Jia W, Leary J, Corbett KD, Moore JK, Al-Bassam J
eLife (2015) 4 [ Pubmed: 26208336 DOI: doi:10.7554/eLife.08811 ]
- Tubulin folding cofactor e, n-terminal gfp fusion (Protein from Saccharomyces cerevisiae)
- Arl2 gtpase, q73l mutant (Protein from Saccharomyces cerevisiae)
- Tubulin folding cofactor d (Protein from Saccharomyces cerevisiae)
- Tubulin cofactor complex [tbc-de(n-gfp)g] (230 kDa, Sample)
Structural basis for TBC-DEG assembly with soluble tubulin dimer
Nithianantham S, Le S, Seto E, Jia W, Leary J, Corbett KD, Moore JK, Al-Bassam J
eLife (2015) 4 [ Pubmed: 26208336 DOI: doi:10.7554/eLife.08811 ]
- Alpha tubulin (Protein from unidentified)
- Tubulin folding cofactor d (Protein from Saccharomyces cerevisiae)
- Tubulin folding cofactor e (Protein from Saccharomyces cerevisiae)
- Tubulin cofactor complex [tbc-de(n-gfp)g] (308 kDa, Sample)
- Arl2 gtpase, q73l mutant (Protein from Saccharomyces cerevisiae)
- Beta tubulin (Protein from unidentified)
Structural basis for TBC-DEG assembly with TBCC and soluble tubulin dimer
Nithianantham S, Le S, Seto E, Jia W, Leary J, Corbett KD, Moore JK, Al-Bassam J
eLife (2015) 4 [ Pubmed: 26208336 DOI: doi:10.7554/eLife.08811 ]
- Alpha tubulin (Protein from unidentified)
- Tubulin cofactor complex (tbc-deg) with tbcc and tubulin dimer (340 kDa, Sample)
- Tubulin folding cofactor d (Protein from Saccharomyces cerevisiae)
- Tubulin folding cofactor e (Protein from Saccharomyces cerevisiae)
- Tubulin binding cofactor c (Protein from Saccharomyces cerevisiae)
- Arl2 gtpase, q73l mutant (Protein from Saccharomyces cerevisiae)
- Beta tubulin (Protein from unidentified)