Cryo-EM structure of microtubule-bound KLP61F motor domain in the AMPPNP state
Resolution: 4.4 Å
EM Method: Helical reconstruction
Fitted PDBs: 6vpo
Q-score: 0.324
Bodrug T, Wilson-Kubalek EM, Nithianantham S, Thompson AF, Alfieri A, Gaska I, Major J, Debs G, Inagaki S, Gutierrez P, Gheber L, McKenney RJ, Sindelar CV, Milligan R, Stumpff J, Rosenfeld SS, Forth ST, Al-Bassam J
eLife (2020) 9 [ Pubmed: 31958056 DOI: doi:10.7554/eLife.51131 ]
- Microtubule (Complex from Sus scrofa)
- Kinesin-like protein klp61f (42 kDa, Protein from Drosophila melanogaster)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1a chain (50 kDa, Protein from Sus scrofa)
- Klp61f motor (Complex from Drosophila melanogaster)
- Microtubule-bound klp61f motor in the amppnp state (Complex)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
Cryo-EM structure of microtubule-bound KLP61F motor with tail domain in the nucleotide-free state
Resolution: 4.4 Å
EM Method: Helical reconstruction
Fitted PDBs: 6vpp
Q-score: 0.218
Bodrug T, Wilson-Kubalek EM, Nithianantham S, Thompson AF, Alfieri A, Gaska I, Major J, Debs G, Inagaki S, Gutierrez P, Gheber L, McKenney RJ, Sindelar CV, Milligan R, Stumpff J, Rosenfeld SS, Forth ST, Al-Bassam J
eLife (2020) 9 [ Pubmed: 31958056 DOI: doi:10.7554/eLife.51131 ]
- Microtubule (Complex from Sus scrofa)
- Kinesin-like protein klp61f (42 kDa, Protein from Drosophila melanogaster)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1a chain (50 kDa, Protein from Sus scrofa)
- Klp61f motor (Complex from Drosophila melanogaster)
- Microtubule-bound klp61f motor with tail domain in the nucleotide-free state (Complex)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
Tubulin cofactors-D,E and Arl2-Q73L GTPase form a stable heterotrimeric chaperone
Nithianantham S, Le S, Seto E, Jia W, Leary J, Corbett KD, Moore JK, Al-Bassam J
eLife (2015) 4 [ DOI: doi:10.7554/eLife.08811 Pubmed: 26208336 ]
Tubulin cofactors-D,E(N-GFP) and Arl2-Q73L GTPase form a stable heterotrimeric chaperone
Nithianantham S, Le S, Seto E, Jia W, Leary J, Corbett KD, Moore JK, Al-Bassam J
eLife (2015) 4 [ DOI: doi:10.7554/eLife.08811 Pubmed: 26208336 ]
- Tubulin folding cofactor e, n-terminal gfp fusion (Protein from Saccharomyces cerevisiae)
- Tubulin folding cofactor d (Protein from Saccharomyces cerevisiae)
- Tubulin cofactor complex [tbc-de(n-gfp)g] (230 kDa, Sample)
- Arl2 gtpase, q73l mutant (Protein from Saccharomyces cerevisiae)
Structural basis for TBC-DEG assembly with soluble tubulin dimer
Nithianantham S, Le S, Seto E, Jia W, Leary J, Corbett KD, Moore JK, Al-Bassam J
eLife (2015) 4 [ DOI: doi:10.7554/eLife.08811 Pubmed: 26208336 ]
- Beta tubulin (Protein from unidentified)
- Alpha tubulin (Protein from unidentified)
- Tubulin cofactor complex [tbc-de(n-gfp)g] (308 kDa, Sample)
- Arl2 gtpase, q73l mutant (Protein from Saccharomyces cerevisiae)
- Tubulin folding cofactor e (Protein from Saccharomyces cerevisiae)
- Tubulin folding cofactor d (Protein from Saccharomyces cerevisiae)
Structural basis for TBC-DEG assembly with TBCC and soluble tubulin dimer
Nithianantham S, Le S, Seto E, Jia W, Leary J, Corbett KD, Moore JK, Al-Bassam J
eLife (2015) 4 [ DOI: doi:10.7554/eLife.08811 Pubmed: 26208336 ]
- Beta tubulin (Protein from unidentified)
- Alpha tubulin (Protein from unidentified)
- Arl2 gtpase, q73l mutant (Protein from Saccharomyces cerevisiae)
- Tubulin folding cofactor e (Protein from Saccharomyces cerevisiae)
- Tubulin folding cofactor d (Protein from Saccharomyces cerevisiae)
- Tubulin binding cofactor c (Protein from Saccharomyces cerevisiae)
- Tubulin cofactor complex (tbc-deg) with tbcc and tubulin dimer (340 kDa, Sample)